High-throughput screening: New technology for the 21st century

Current Opinion in Chemical Biology

Volumn 4, Issue 4, 2000, Pages 445-451

  Hertzberg, Robert P ^a   Pope, Andrew J ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BETA LACTAMASE; CYCLIC AMP; G PROTEIN COUPLED RECEPTOR; LUCIFERASE; PROGESTERONE RECEPTOR; PROTEIN KINASE;

ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ANISOTROPY; DRUG RESEARCH; DRUG SCREENING; ENERGY TRANSFER; ENZYME ASSAY; FLUORESCENCE ANALYSIS; FLUORESCENCE SPECTROSCOPY; HUMAN; IRON TRANSPORT; MOLECULAR BIOLOGY; NONHUMAN; PROTEIN PROTEIN INTERACTION; RADIOENZYMATIC ASSAY; REVIEW; SCINTILLATION;

EID: 0033863669     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(00)00110-1     Document Type: Review

References (83)

1. Assay miniaturization for ultra-high throughput screening of combinatorial and discrete compound libraries: A 9600-well (0.2 microliter) assay system
2. Digital imaging as a detection method for a fluorescent protease assay in 96-well and miniaturized assay plate formats
3. Homogeneous fluorescence readouts for miniaturized high-throughput screening; Theory and practice
4. Macroscopic versus microscopic fluorescence techniques in (ultra)-high-throughput screening
5. Miniaturization of homogeneous assays using fluorescence polarization
6. Imaging systems in assay screening
7. High throughput fluorescence polarization: A homogeneous alternative to radioligand binding for cell surface receptors
8. Development of high throughput screening assays using fluorescence polarization: Nuclear receptor-ligand-binding and kinase/phosphatase assays
9. An ultra-high throughput screening approach for an adenine transferase using fluorescence polarization
10. HTRF technology
11. LANCE(TM): Homogeneous assay platform for HTS
12. Nuclear receptors have distinct affinities for coactivators: Characterization by fluorescence resonance energy transfer
13. Time-resolved energy transfer DNA helicase assays for high throughput screening
14. A homogeneous 384-well high throughput screen for novel tumor necrosis factor receptor: Ligand interactions using time-resolved energy transfer
15. A bioluminescence resonance energy transfer (BRET) system: Applications to interacting circadian clock proteins
16. Fluorescence-lifetime technologies for high-throughput screening
17. A time resolved fluorimeter for high-throughput screening
18. High throughput screening with multiphoton excitation
19. Time resolved fluorescence measurements of actin phalloidin interactions
20. Semiconductor nanocrystals as fluorescent biological labels
21. Quantum dot bioconjugates for ultrasensitive nonisotopic detection
22. Fluorescence correlation spectroscopy: Lead discovery by miniaturized HTS
23. Single molecule detection technologies in high throughput screening: Fluorescence correlation spectroscopy
24. Fluorescence correlation spectroscopy-based screening technology
25. Fluorescence-intensity distribution analysis and its application in biomolecular detection technology
26. The photon counting histogram in fluorescence fluctuation spectroscopy
27. Resolving heterogeneity on the single molecular level with the photon counting histogram
28. Quantitative identification of different single molecules be selective time-resolved confocal fluorescence spectroscopy
29. Rapid assay processing by integration of dual-color fluorescence correlation spectroscopy: High throughput screening for enzyme activity
30. Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy
31. Confocal fluorescence coincidence analysis: An approach to ultra high-throughput screening
32. Scintillation proximity assays - A versatile high throughput screening technology
33. A scintillation proximity-assay for the Raf/MEK/ERK kinase cascade: High-throughput screening and identification of selective enzyme inhibitors
34. Homogeneous proximity tyrosine kinase assays: Scintillation proximity assay versus homogeneous time-resolved fluorescence
35. A homogeneous method to measure aminoacyl-tRNA synthetase activities using scintillation proximity assay technology
36. Development of a scintillation proximity assay for histone deacetylase using a biotinylated peptide derived from histone H4
37. Validation of a high throughput scintillation proximity assay for 5-hydroxytryptamine(1E) receptor binding activity
38. High throughput scintillation proximity assay for the identification of FKBP-12 ligands
39. A screening strategy based on differential binding of ligand to receptor and to binding proteins: Screening for compounds interacting with corticotrophin-releasing factor-binding protein
40. High throughput quantitation of cAMP production mediated by activation of seven transmembrane domain receptors
41. Development of non-separation binding and functional assays for G protein-coupled receptors for high throughput screening: Pharmacological characterization of the immobilized CCR5 receptor on FlashPlate(TM)
42. FLIPR: A new instrument for accurate, high throughput optical screening
43. Cell-based assays and instrumentation for screening ion-channel targets
44. Melanin-concentrating hormone is the cognate ligand for the orphan G-protein-coupled receptor SLC-1
45. The endogenous lipid anandamide is a full agonist at the human vanilloid receptor (hVR1)
46. Functional characterization of bradykinin analogues on recombinant human bradykinin B-1 and B-2 receptors
47. Fluorometric imaging plate reader: Using the orexin receptor to screen 145,000 compounds
48. Fluorescence techniques for measuring ion channel activity
49. Miniaturization of a mammalian cell-based assay: Luciferase reporter gene readout in a 3 microliter 1536-well plate
50. A 1536 colorimetric SPAP reporter assay: Comparison with 96- and 384-well formats
51. Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter
52. Expression of Cre recombinase as a reporter of signal transduction in mammalian cells
53. Identification of surrogate agonists for the human FPRL-1 receptor by autocrine selection in yeast
54. Miniaturization of a functional transcription assay in yeast (human progesterone receptor) in the 384- and 1536-well plate format
55. A melanophore based screening assay for erythropoietin receptors
56. A genetically encoded fluorescent indicator for cyclic AMP in living cells
57. Quantification of G-protein coupled receptor internalization using G-protein coupled receptor-green fluorescent protein conjugates with the ArrayScan(TM) high-content screening system
58. High-content screening: A new approach to easing key bottlenecks in the drug discovery process
59. Ligand-receptor binding measured by laser-scanning imaging
60. Determination of ligand binding affinities for endogenous seven-transmembrane receptors using fluorometric microvolume assay technology
61. Optical sectioning fluorescence spectroscopy in a programmable array microscope
62. Miniaturized FRET assays and microfluidics: Key components for ultra-high-throughput screening
63. Microdispensing technologies in drug discovery
64. The evolution of miniaturized well plates
65. Test tube's end
66. Microfluidic assays for high-throughput submicroliter assays using capillary electrophoresis
67. Monolithic microfabricated valves and pumps by multilayer soft lithography