A homogeneous method to measure aminoacyl-tRNA synthetase aminoacylation activity using scintillation proximity assay technology

Analytical Biochemistry

Volumn 284, Issue 2, 2000, Pages 183-190

  Macarrón, Ricardo ^a   Mensah, Lucy ^a   Cid, Concha ^a   Carranza, Carmen ^a   Benson, Neil ^a   Pope, Andrew J ^a   Díez, Emilio ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Aminoacyl tRNA synthetase; EC6.1.1; Enzyme inhibitors; High throughput screening; Radioisotope assays; SPA technology

Indexed keywords

AMINO ACIDS; ENZYMES; PRECIPITATION (METEOROLOGY); RATE CONSTANTS; SILICATES; TRICHLOROACETIC ACID; YTTRIUM COMPOUNDS;

COUNTING EFFICIENCY; EC6.1.1; ENZYME INHIBITORS; HIGH THROUGHPUT SCREENING; SIGNAL DISCRIMINATION; STATISTICAL PERFORMANCE; STEADY-STATE KINETIC CONSTANTS; SYNTHETASES;

SCINTILLATION;

AMINO ACID; AMINO ACID TRANSFER RNA LIGASE; TRANSFER RNA; TRICHLOROACETIC ACID; YTTRIUM;

ACID PRECIPITATION; AMINOACYLATION; ARTICLE; BINDING AFFINITY; ENZYME ACTIVITY; ENZYME ANALYSIS; ISOTOPE LABELING; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RNA BINDING; SCINTISCANNING; SCREENING; SIGNAL DETECTION; STEADY STATE;

EID: 0034633290     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4665     Document Type: Article

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