Development of nonseparation binding and functional assays for G protein-coupled receptors for high throughput screening: Pharmacological characterization of the immobilized CCR5 receptor on FlashPlate®

Journal of Biomolecular Screening

Volumn 3, Issue 4, 1998, Pages 285-292

  Bossé, Roger ^a   Garlick, Russell ^b   Brown, Beverly ^b   Ménard, Luc ^a  

^a INSTITUTE FOR RESEARCH IN IMMUNOLOGY AND CANCER   (Canada)

^b NEN Life Science Products   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001446284     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705719800300407     Document Type: Article

References (16)

1. Udenfriend, S., Gerber, L., Nelson, N. (1987). Scintillation proximity assay: a sensitive and continuous isotopic method for monitoring ligand/receptor and antigen/antibody interactions. Anal. Biochem. 161:494-500.
2. Patel, S., Harris, A., O'Beirne, G., Cook, N.D., Taylor, C.W. (1996). Kinetic analysis of inositol trisphosphate binding to pure inositol trisphosphate receptors using scintillation proximity assay. Biochem. Biophys. Res. Commun: 221:821-825.
3. Holland, J.D., Singh, P., Brennand, J.C., Garman, A.J. (1994). A non separation microplate receptor binding assay. Anal. Biochem. 222:516-518.
4. TM technology. In High Throughput Screening. The Discovery of Bioactive Substances. J.P. Devlin (ed.), pp. 317-328, Marcel Dekker, New York.
5. Gorman, C., Skinner, R.H., Skelly, J.V., Neidle, S., Lowe, P.N. (1996). Equilibrium and kinetic measurements reveal rapidly reversible binding of Ras to Raf. J. Biol. Chem. 271:6713-6719.
6. Lerner, C.G., Saiki, A.Y.C. (1996). Scintillation proximity assay for human DNA topoisomerase I using recombinant biotinyl-fusion protein produced in baculovirus-infected insect cells. Anal. Biochem. 240:185-196.
7. Watson, S., Arkinstall, S. (1994). The G-protein Linked Receptor Facts Book. Academic Press, San Diego, CA.
8. Dutton, G. (1997). Genetic Engineering News, April 8, pp. 33-34.
9. Feng, Y., Broder, C.C., Kennedy, P.E., Berger, E.A. (1996). HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 272:872-877.
10. Alkhatib, G., Combadiere, C., Broder, C.C., Feng, Y., Kennedy, P.E., Murphy, P.M., Berger, E.A. (1996). CC CKR5: A RANTES, MIP-1α, MIP-1b receptor as a fusion cofactor for macrophagetropic HIV-1. Science. 272:1955-1958.
11. Choe, H., Farzan, M., Sun, Y., Sullivan, N., Rollins, B., Ponath, P.D., Wu, L., Mackay, C.R., LaRosa, G., Newman, W., Gerard, N., Gerard, C., Sodroski, J. (1996). The β-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 85:1135-1148.
12. Doranz, B J., Rucker, J., Yi, Y., Smyth, R.J., Samson, M., Peiper, S.C., Parmentier, M., Collman, R.G., Doms, R.W. (1996). A dualtropic primary HIV-1 isolate that uses fusin and the β-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors. Cell. 85:1149-1158.
13. Wu, L., Gerard, N.P., Wyatt, R., Choe, H., Parolin, C., Ruffing, N., Borsetti, A., Cardoso, A.A., Desjardin, E., Newman, W., Gerard, C., Sodroski, J. (1996). CD4-indiced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5. Nature. 384:179-183.
14. Oberlin, E., Amara, A., Bachelerie, F., Bessia, C., Virelizier, J.-L., Arenzana-Seisdedos, F., Schwanz, O., Heard, J.-M., Clark-Lewis, I., Legler, D.F., Loetscher, M., Baggiolini, M., Moser, B. (1996). The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 382:833-835.
15. Combadiere, C., Ahuja, S.K., Tiffany, H.L., Murphy, P.M. (1996). Cloning and functional expression of CC CKR5, a human monocyte CC chemokine receptor selective for MOM (alpha), MIP-1(beta), and RANTES. J. Leukoc. Biol. 60:147-152.
16. Liu, R., Paxton, W.A., Choe, S., Ceradini, D., Martin, S.R., Horuk, R., MacDonald, M.E., Stuhlmann, H., Koup, R., Landau, N.R. (1996). Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection. Cell. 86:367-377.