Quantitation of transcription and clonal selection of single living cells with β-lactamase as reporter

Science

Volumn 279, Issue 5347, 1998, Pages 84-88

  Zlokarnik, Gregor ^a   Negulescu, Paul A ^a   Knapp, Thomas E ^a   Mere, Lora ^a   Burres, Neal ^a   Feng, Luxin ^a   Whitney, Michael ^a   Roemer, Klaus ^a   Tsien, Roger Y ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE; AMPICILLIN; ANTIBIOTIC AGENT; ARECOLINE; BACTERIAL ENZYME; BETA LACTAM ANTIBIOTIC; BETA LACTAMASE; BETHANECHOL; CARBACHOL; METHACHOLINE; MUSCARINIC AGENT; MUSCARINIC RECEPTOR BLOCKING AGENT; PILOCARPINE; PIRENZEPINE;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; MAMMAL CELL; MEMBRANE PERMEABILITY; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE;

ANIMALS; BETA-LACTAMASES; BETA-LACTAMS; CELL LINE; CELL SEPARATION; CLONE CELLS; DNA-BINDING PROTEINS; DRUG EVALUATION, PRECLINICAL; ENERGY TRANSFER; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; GENE EXPRESSION; GENES, REPORTER; HALF-LIFE; HUMANS; LACTAMS; MUSCARINIC AGONISTS; MUSCARINIC ANTAGONISTS; NFATC TRANSCRIPTION FACTORS; NUCLEAR PROTEINS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRANSFECTION; TUMOR CELLS, CULTURED; UMBELLIFERONES;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 0032472195     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5347.84     Document Type: Article

References (27)

1. J. Alam and J. L. Cook, Anal. Biochem. 188, 245 (1990).
2. A. B. Cubitt et al., Trends Biochem. Sci. 20, 448 (1995).
3. H. Christensen, M. T. Martin, S. G. Waley, Biochem. J. 266, 853 (1990).
4. M. I. Page, Adv. Phys. Org. Chem. 23, 165 (1987).
5. R. P. Ambler, Philos. Trans. R. Soc. London Ser. B 289, 321 (1980).
6. J. G. Sutcliffe, Proc. Natl. Acad. Sci. U.S.A. 75, 3737 (1978).
7. J. T. Kadonaga et al., J. Biol. Chem. 259, 2149 (1984).
8. C. H. O'Callaghan, A. Morris, S. M. Kirby, A. H. Shingler, Antimicrob. Agents Chemother. 1, 283 (1972).
9. R. N. Jones, H. W. Wilson, W. J. Novick Jr., J. Clin. Microbiol. 15, 677 (1982).
10. _, A. L. Barry, C. Thornsberry, ibid., p. 954.
11. J. T. Moore, S. T. Davis, I. K. Dev, Anal. Biochem. 247, 203 (1997).
12. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983).
13. R. Y. Tsien and G. Zlokarnik, patent application WO96/30540 (1996). Inquiries from nonprofit institutions regarding commerical availability of CCF2/AM should be addressed to Packard Instrument, 800 Research Parkway, Meriden, CT 06450.
14. E. Wingender and A. Arellano, Anal. Biochem. 127, 351 (1982).
15. F. Di Virgilio, T. H. Steinberg, J. A. Swanson, S. C. Silverstein, J. Immunol. 140, 915 (1988).
16. S. Rering et al., Genes Dev. 4, 1823 (1990).
17. J. Karttunen and N. Shastri, Proc. Natl. Acad. Sci. U.S.A. 88, 3972 (1991).
18. Assays of glucocorticoid signaling with receptors have generally required transfection of exogenous glucocorticoid receptors to achieve adequate sensitivity [V. Giguère, S. M. Hollenberg, M. G. Rosenfeld, R. M. Evans, Cell 46, 645 (1986)].
19. FACS has been used to select cell lines in which β-Gal expression was switched off [A. T. Serafini et al., Immunity 3, 239 (1995)].
20. As further evidence against toxicity of the β-lactamase substrate, microinjection of CCF2 into zebrafish embryos at the single-cell stage produced no developmental abnormalities even though the embryos remained strongly fluorescent after 30 hours (E. Raz and W. Driever, personal communication).
21. H. Weintraub, Proc. Natl. Acad. Sci. U.S.A. 85, 5819 (1988).
22. H. H. McAdams and A. Arkin, ibid. 94, 814 (1997).
23. P. A. Negulescu, N. Shastri, M. D. Cahalan, ibid. 91, 2873 (1994).
24. J. F. Thompson, L. S. Hayes, D. B. Lloyd, Gene 103, 171 (1991).
25. A. Matagne et al., Biophys. J. 265, 131 (1990).
26. K. D. Niswender, S. M. Blackman, L. Rohde, M. A. Magnuson, D. W. Piston, J. Microsc. 180, 109 (1995).
27. 1 receptor gene, and S. Ho and G. Crabtree for a plasmid containing a tandem NF-AT-binding site trimer. The work in the Tsien lab was supported by NIH grant NS27177 and the Howard Hughes Medical Institute.