Time-resolved fluorescence energy transfer DNA helicase assays for high throughput screening

Journal of Biomolecular Screening

Volumn 4, Issue 5, 1999, Pages 239-248

  Earnshaw, David L ^a   Moore, Keith J ^a   Greenwood, Catherine J ^a   Djaballah, Hakim ^a   Jurewicz, Anthony J ^a   Murray, Kenneth J ^a   Pope, Andrew J ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME INHIBITOR; HELICASE; RHODAMINE; TERBIUM;

ARTICLE; BACTERIUM; DNA DENATURATION; DRUG SCREENING; ENZYME ASSAY; FLUORESCENCE ANALYSIS; PRIORITY JOURNAL; RECOMBINANT DNA TECHNOLOGY; VIRUS;

BACTERIA (MICROORGANISMS);

EID: 0032721827     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705719900400505     Document Type: Article

References (10)

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3. Pan, X.S. and Fisher, L.M. (1998). DNA gyrase and topoisomerase IV are dual targets of clinafloxacin action in Streptococcus pneumoniae. Antimicrob. Agents Chemother. 42:2810-2816.
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5. Spector, F.C., Liang, L., Giordano, H., Sivaraja, M., Peterson, M.G. (1998). Inhibition of herpes simplex virus replication by a 2-amino thiazole via interactions with the helicase component of the UL5-UL8-UL52 complex. J. Virol. 72:6879-6987.
6. Amaratunga, M., Lohman, T.M. (1993). Escherichia coli Rep helicase unwinds DNA by an active mechanism. Biochemistry 32:6815-6820.
7. Bjornson, K.P., Amaratunga, M., Moore, K.J., Lohman T.M. (1994). Single-turnover kinetics of helicase-catalysed DNA unwinding monitored continuously by fluorescence energy transfer. Biochemistry 33:14306-14316.
8. Lohman, T.M., Bjornson, K.P. (1996). Mechanisms of helicase-catalysed DNA unwinding. Annu. Rev. Biochem. 65:169-214.
9. Houston, P., Kodadek, T. (1994). Spectrophotometric assay for enzyme-mediated unwinding of double-stranded DNA. Proc. Natl. Acad. Sc. USA 91:5471-5474.
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