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Volumn 288, Issue 5463, 2000, Pages 113-116

Monolithic microfabricated valves and pumps by multilayer soft lithography

Author keywords

[No Author keywords available]

Indexed keywords

ELASTOMER; SILICON;

EID: 0034615958     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5463.113     Document Type: Article
Times cited : (3632)

References (42)
  • 14
    • 8944257381 scopus 로고    scopus 로고
    • Y. N. Xia et al., Science 273, 347 (1996).
    • (1996) Science , vol.273 , pp. 347
    • Xia, Y.N.1
  • 23
    • 0342811863 scopus 로고    scopus 로고
    • note
    • For multilayers, a thick layer was prepared as previously described; each thin layer was baked at 80°C for 20 min. The growing thick layer was assembled on each new thin layer and bonded by baking at 80°C for 20 min. Seven-layer devices have been produced by this method; no obvious limitations exist to limit the number of layers.
  • 25
    • 0343682161 scopus 로고    scopus 로고
    • note
    • -1. Magnetic silicone was created by the addition of iron powder (∼ 1 μm particle size); up to 20% Fe by weight was added. For both conductive and magnetic silicones, multilayer bonding functioned normally.
  • 34
    • 0343246501 scopus 로고    scopus 로고
    • note
    • The magnitude of flow (and even its direction) depends in a complicated fashion on ionic strength and type, the presence of surfactants, and the charge on the walls of the flow channel; furthermore, because electrolysis is taking place continuously, the capacity of buffer to resist pH changes is finite. Precise control of flow thus requires calibration for each new buffer or solute and can be difficult when the exact composition of a sample is not known in advance. Electroosmotic flow can also induce unwanted electrophoretic separation of molecules, creating demixing problems. Dielectrophoresis does not require electrolysis and therefore does not cause bubble formation but still suffers from sample and solvent sensitivity.
  • 35
    • 0343246500 scopus 로고    scopus 로고
    • note
    • Each control channel was connected to the common port of a miniature three-way switch valve (LHDA1211111H; Lee Valve, Westbrook, CT), powered by a fast Zener-diode circuit and controlled by a digital data acquisition card (AT-DIO-32HS; National Instruments, Austin, TX). Regulated external pressure was provided to the normally closed port, allowing the control channel to be pressurized or vented to atmosphere by switching the miniature valve.
  • 36
    • 0343246499 scopus 로고    scopus 로고
    • note
    • If one used another actuation method that did not suffer from opening and closing lag, this valve would run at ∼375 Hz. The spring constant can be adjusted by changing the membrane thickness; this allows optimization for either fast opening or fast closing.
  • 37
    • 0342376953 scopus 로고    scopus 로고
    • note
    • E. coli were pumped at 10 Hz through the channel. Samples of known volume were taken from the output well (pumped) and the input well (control), and serial dilutions of each were plated on Luria-Bertani agar plates and grown overnight at 37°C. Viability was assessed by counting colonies in the control and pumped samples and correcting for sample volumes and dilution.
  • 42
    • 0342811862 scopus 로고    scopus 로고
    • note
    • This work was partially supported by NIH (NS-11756, DA-9121).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.