Efficient purification of plasmid DNA for gene transfer using triple-helix affinity chromatography

Gene Therapy

Volumn 4, Issue 4, 1997, Pages 323-330

  Wils, P ^a   Escriou, V ^a   Warnery, A ^a   Lacroix, F ^a   Lagneaux, D ^a   Ollivier, M ^a   Crouzet, J ^a   Mayaux, J F ^a   Scherman, D ^a  

^a SANOFI   (France)

Author keywords

Affinity chromatography; DNA purification; DNA triplex; DNA vaccines; Gene therapy; Triple helix

Indexed keywords

DNA; ENDOTOXIN; OLIGONUCLEOTIDE; PLASMID DNA; RNA;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; COVALENT BOND; DNA DNA HYBRIDIZATION; DNA PURIFICATION; DNA SEQUENCE; DNA SUPERCOILING; GENE TRANSFER; METHODOLOGY; NONHUMAN; PH; PRIORITY JOURNAL; TRIPLE HELIX;

EID: 0030979103     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300388     Document Type: Article

References (19)

1. Gao X, Huang L. Cationic liposome-mediated gene transfer. Gene Therapy 1995; 2: 710-722.
2. Donnelly JJ, Ulmer JB, Liu MA. Immunisation with DNA. J Immunol Meth 1994; 176: 145-152.
3. Caplen NJ et al. Gene therapy for cystic fibrosis in humans by liposome-mediated DNA transfer: the production of resources and the regulatory process. Gene Therapy 1994; 1: 139-147.
4. Horn NA, Meek JA, Budahazi G, Marquet M. Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials. Hum Gene Ther 1995; 6: 565-573.
5. Thompson JA. A review of high-performance liquid chromatography in nucleic acids research. III. Isolation, purification, and analysis of supercoiled plasmid DNA. BioChromatography 1986; 1: 68-80.
6. Sun JS, Hélène C. Oligonucleotide-directed triple-helix formation. Curr Opin Struct Biol 1993; 3: 345-356.
7. Escriou V et al. Triple helix formation on plasmid DNA determined by a size exclusion Chromatographic method (submitted for publication).
8. Rougée M et al. Kinetics and thermodynamics of triple-helix formation: effects of ionic strength and mismatches. Biochemistry 1992; 31: 9269-9278.
9. Ito T, Smith CL, Cantor CR. Sequence-specific DNA purification by triplex affinity capture. Proc Natl Acad Sci USA 1992; 89: 495-498.
10. Ji H, Smith LM. Rapid purification of double-stranded DNA by triple-helix-mediated affinity capture. Anal Chem 1993, 65: 1323-1328.
11. Kiessling LL, Griffin LC, Dervan PB. Flanking sequence effects within the pyrimidine triple-helix motif characterized by affinity cleaving. Biochemistry 1992; 31: 2829-2834.
12. Maher LJ III, Dervan PB, Wold BJ. Kinetic analysis of oligodeoxyribonucleotide-directed triple-helix formation on DNA. Biochemistry 1990; 29: 8820-8826.
13. Morrison DC, Ryan JL. Endotoxins and disease mechanisms. Ann Rev Med 1987; 38: 417-432.
14. Gotten M et al. Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus. Gene Therapy 1994; 1: 239-246.
15. Wicks IP et al. Bacterial lipopolysaccharide copurifies with plasmid DNA: implications for animal models and human gene therapy. Hum Gene Ther 1995; 6: 317-323.
16. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press. New York, 1989.
17. Obi FO. Interaction of DNA with hydroxyapatite. Studies on the effect of the phosphate concentration of the column equilibration and washing buffer. J Chromatogr 1986; 369: 321-326.
18. Debouck C et al. Structure of the galactokinase gene of Escherichia coli, the last gene of the gal operon. Nucleic Acids Res 1985; 13: 1841-1853.
19. Byk G, Dubertret C, Scherman D. Lipopolyamines as transfection agents and pharmaceutical uses thereof. World Patent 1996, WO 96/17823.