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48
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9544226448
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A humanized system for pharmacologic control of gene expression
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Rivera VM, Clackson T, Natesan S, Pollock R, Amara J, Keenan T, Magari SR, Phillips T, Courage NL, Cerasoli F et al.: A humanized system for pharmacologic control of gene expression. Nat Med 1996, 2:1028-1032. This work uses the natural heterodimerizing chemical inducer of dimerization rapamycin as the basis of an inducible system tailored for gene therapy applications. Its properties are explored in vitro, and in mice transplanted with cells stably expressing the components of this scheme.
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(1996)
Nat Med
, vol.2
, pp. 1028-1032
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Rivera, V.M.1
Clackson, T.2
Natesan, S.3
Pollock, R.4
Amara, J.5
Keenan, T.6
Magari, S.R.7
Phillips, T.8
Courage, N.L.9
Cerasoli, F.10
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49
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85030302867
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1997 April; Snowbird, Utah. Silverthorne, Colorado: Keystone Symposia Organisation
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Rivera V, Clackson T, Natesan S, Pollock R, Amara J, Keenan T, Rozamus L, Gilman M: Humanized system for pharmacologic control of gene expression. Keystone Symposia on Molecular and Cellular Biology of Gene Therapy: 1997 April; Snowbird, Utah. Silverthorne, Colorado: Keystone Symposia Organisation; 1997.
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(1997)
Humanized System for Pharmacologic Control of Gene Expression. Keystone Symposia on Molecular and Cellular Biology of Gene Therapy
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Rivera, V.1
Clackson, T.2
Natesan, S.3
Pollock, R.4
Amara, J.5
Keenan, T.6
Rozamus, L.7
Gilman, M.8
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50
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0028948325
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Structure-based design of transcription factors
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Pomerantz JL, Sharp PA, Pabo CO: Structure-based design of transcription factors. Science 1995, 267:93-96.
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(1995)
Science
, vol.267
, pp. 93-96
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Pomerantz, J.L.1
Sharp, P.A.2
Pabo, C.O.3
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51
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33748226936
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Rational design of orthogonal receptor-ligand combinations
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Belshaw PJ, Schoepfer JG, Liu KQ, Morrison KL, Schreiber SL: Rational design of orthogonal receptor-ligand combinations. Angew Chem Int Ed 1995, 34:2129-2132.
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(1995)
Angew Chem Int Ed
, vol.34
, pp. 2129-2132
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Belshaw, P.J.1
Schoepfer, J.G.2
Liu, K.Q.3
Morrison, K.L.4
Schreiber, S.L.5
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52
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0030878873
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Inducible gene expression and protein translocation using nontoxic ligands identified by a mammalian three-hybrid screen
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Liberles SD, Diver ST, Austin DJ, Schreiber SL: Inducible gene expression and protein translocation using nontoxic ligands identified by a mammalian three-hybrid screen. Proc Natl Acad Sci USA 1997, 94:7825-7830. This paper describes the synthesis of nontoxic rapamycin derivatives, employing a mammalian three-hybrid-based assay to discover mutant forms of FRAP that can interact with these safer analogs of rapamycin. These novel receptor-ligand complexes are used to control gene expression and signal transduction.
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(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 7825-7830
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Liberles, S.D.1
Diver, S.T.2
Austin, D.J.3
Schreiber, S.L.4
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54
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0029737867
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Dimeric ligands define a role for transcriptional activation domains in reinitiation
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Ho SN, Biggar SR, Spencer DM, Schreiber SL, Crabtree GR: Dimeric ligands define a role for transcriptional activation domains in reinitiation. Nature 1996, 382:822-826. Interesting application of CID technology that makes use of the monomeric forms to speed up disruption of CID-immunophilin complexes. It also contains useful information regarding the kinetics of these interactions.
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(1996)
Nature
, vol.382
, pp. 822-826
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Ho, S.N.1
Biggar, S.R.2
Spencer, D.M.3
Schreiber, S.L.4
Crabtree, G.R.5
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