A plant-based cholera toxin B subunit insulin fusion protein protects against the development of autoimmune diabetes

Nature Biotechnology

Volumn 16, Issue 10, 1998, Pages 934-938

  Arakawa, Takeshi ^a   Yu, Jie ^a   Chong, Daniel K X ^a   Hough, John ^a   Engen, Paul C ^a   Langridge, William H R ^a  

^a LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Insulin dependent diabetes mellitus; Oral tolerance; Potato; Vaccine development

Indexed keywords

AUTOANTIGEN; CHOLERA TOXIN B SUBUNIT; GANGLIOSIDE GM1; HYBRID PROTEIN;

ANTIGENICITY; ARTICLE; AUTOIMMUNITY; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEEDING; IMMUNOBLOTTING; IMMUNOLOGICAL TOLERANCE; INSULIN DEPENDENT DIABETES MELLITUS; INSULITIS; PLANT; PRIORITY JOURNAL;

ANIMALS; CHOLERA TOXIN; DIABETES MELLITUS, TYPE 1; HUMANS; INSULIN; MICE; MICE, INBRED NOD; PLANTS, GENETICALLY MODIFIED; RECOMBINANT FUSION PROTEINS; SOLANUM TUBEROSUM;

ANIMALIA; SOLANUM TUBEROSUM;

EID: 0031762753     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt1098-934     Document Type: Article

References (32)

1. Eisenbarth, G. 1986. Type I diabetes mellitus: a chronic autoimmune disease. N. Engl. J. Med. 314:1360-1368.
2. Tisch, R. and McDevitt, H. 1996. Insulin-dependent diabetes mellitus. Cell 85:291-297.
3. Zhang, J., Davidson, L., Eisenbarth, G., and Weiner, H.L. 1991. Suppression of diabetes in nonobese diabetic mice by oral administration of porcine insulin. Proc. Natl. Acad. Sci. USA 88:10252-10256.
4. Trentham, D.E., Dynesius-Trentham, R.A., Orav, E.J., Combitchi, D., Lorenzo, C., Sewell, K.L. et al. 1993. Effects of oral administration of type II collagen on rheumatoid arthritis. Science 261:1727-1730.
5. Welner, H.L., Mackin, G.A., Matsui, M., Orav, E.J., Khoury, S.J., Dawson, D.M. et al. 1993. Double-blind pilot trial of oral tolerization with myelin antigens in multiple sclerosis. Science 259:1321-1324.
6. Weiner, H.L., Friedman, A., Miller, A., Khoury, S.J., Al-Sabbagh, A., Santos, L. et al. 1994. Oral tolerance: Immunologic mechanisms and treatment of animal and human organ-specific autoimmune diseases by oral administration of autoantigens. Annu. Rev. Immunol. 12:809-837.
7. Hancock, W.W., Polanski, M., Zhang, J., Blogg, N., and Weiner, H.L. 1995. Suppression of insulitis in non-obese diabetic (NOD) mice by oral insulin administration is associated with selective expression of interleukin-4 and -10, trans-forming growth factor-β, and prostaglandin-E. Am. J. Pathol. 147:1193-1199.
8. Sun, J.-B., Holmgren, J., and Czerkinsky, C. 1994. Cholera toxin B subunit: an efficient transmucosal carrier-delivery system for induction of peripheral immunological tolerance. Proc. Natl. Acad. Sci. USA 91:10795-10799.
9. Sun, J.-B., Rask, C., Olsson, T., Holmgren, J., and Czerkinsky, C. 1996. Treatment of experimental autoimmune encephalomyelitis by feeding myelin basic protein conjugated to cholera toxin B subunit. Proc. Natl. Acad. Sci. USA 93:7196-7201.
10. Bergerot, I., Ploix, C., Petersen, J., Moulin, V., Rask, C., Fabien, N. et al. 1997. A cholera toxoid-insulin conjugate as an oral vaccine against spontaneous autoimmune diabetes. Proc. Natt. Acad. Sci. USA 94:4610-4614.
11. Weiner, H.L. 1994. Oral tolerance. Proc. Natl. Acad. Sci. U S A 91:10762-10765.
12. Haq, T.A., Mason, H.S., Clements, J.D., and Arntzen, C.J. 1995. Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268:714-716.
13. Mason, H.S., Ball, J.M., Shi, J.-J., Jiang, X., Estes, M.K., and Arntzen, C.J. 1996. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogeniclty in mice. Proc. Natl. Acad. Sci. USA 93:5335-5340.
14. Arakawa, T., Chong, D.K.X., and Langridge, W.H.R. 1998. Efficacy of a food plant-based oral cholera toxin B subunit vaccine. Nat. Biotechnol. 16:292-297.
15. Arakawa, T., Chong, D.K.X., Merritt, J.L., and Langridge, W.H.R. 1997. Expression of cholera toxin B subunit oligomers in transgenic potato plants. Transgenic Res. 8:403-413.
16. Ma, S.-W., Zhao, D.-L., Yin, Z.-Q., Mukherjee, R., Singh, B., Qin, H.-Y. et al. 1997. Transgenic plants expressing autoantigens fed to mice to induce oral tolerance. Nat. Med. 3:793-796.
17. Lipscombe, M., Charles, I.Q., Roberts, M., Dougan, G., Tite, J., and Fairweather, N.F. 1991. Intranasal immunization using the B subunit of the Escherichia coli heat-labile toxin fused to an epitope of the Bordetella pertussis P. 69 antigen. Mol. Microbiol. 5:1385-1392.
18. Purvis, I.J., Bettany, A.J., Santiago, T.C., Coggins, J.R., Duncan, K., Eason, R. et al. 1987. The efficiency of folding of some proteins is increased by controlled rates of translation in vivo. A hypothesis. J. Mol. Biol. 193:413-417.
19. von Heljne, G. 1985. Signal sequences. J. Mol. Biol. 184:99-105.
20. Munro, S. and Pelham, H.R.B. 1987. A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899-907.
21. Elson, C.O. and Zivny, J. 1996. Oral tolerance: a commentary, pp. 543-554, in Essentials of mucosal immunology. Academic Press, New York.
22. Challacombe, S.J. and Tomasi, T.B. 1980. Systemic tolerance and secretory immunity after oral immunization. J. Exp. Med. 152:1459-1472.
23. Husby, S., Mestecky, J., Moldoveanu, Z., Holland, S., and Elson, C.O. 1994. Oral tolerance in humans. T cell but not B cell tolerance after antigen feeding. J. Immunol. 152:4663-4670.
24. Fujihasni, K., McGhee, J.R., Yamamoto, M., Hiroi, T., and Kiyono, H. 1996. Role of gamma delta T cells in the regulation of mucosal IgA response and oral tolerance. Ann. N.Y. Acad. Sci. 778:55-63.
25. Chen, Y., Inobe, J., Marks, R., Gonnella, P., Kuchroo, V.K., and Weiner, H.L. 1995. Peripheral deletion of antigen-reactive T cells in oral tolerance. Nature 376:177-180.
26. Kim, P.-H., Eckmann, L., Lee, W.-J., Han, W., and Kagnoff, M.F. 1998. Cholera toxin and cholera toxin B subunit induce IgA switching through the action of TGF-1. J. Immunol. 160:1198-1203.
27. Koncz, C., Olsson, O., Langridge, W.H.R., Schell, J., and Szalay, A.A. 1987. Expression and assembly of functional bacterial luciferase in plants. Proc. Natl. Acad. Sci. USA 84:131-135.
28. Langridge, W.H.R., Fitzgerard, K.L., Koncz, C., Schell, J., and Szalay, A.A. 1989. Dual promoter of Agrobacterium tumefaciens mannopine synthase gene is regulated by plant growth hormones. Proc. Natl. Acad. Sci. USA 86:3219-3223.
29. Kozak, M. 1981. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 9:5233-5252.
30. Wandelt, C.I., Khan, M.R.I., Craig, S., Schroeder, H.E., Spencer, D., and Higgins, T.J.V. 1992. Vicilin with carboxy-terminal KDEL is retained in the endoplasmic reticulum and accumulates to high levels in the leaves of transgenic plants. Plant J. 2:181-192.
31. Doyle, J.J. and Doyle, J.L. 1992. Isolation of plant DNA from fresh tissue. Focus 12:135.
32. Escher, A., O'Kane, D.J., Lee, J., and Szalay, A.A. 1989. Bacterial luciferase alpha-beta fusion protein is fully active as a monomer and highly sensitive in vivo to elevated temperature. Proc. Natl. Acad. Sci. USA 86:6528-6532.