Modifying the Insect Cell N-Glycosylation Pathway with Immediate Early Baculovirus Expression Vectors

Nature Biotechnology

Volumn 14, Issue 10, 1996, Pages 1288-1292

  Jarvis, Donald L ^a   Finn, Eric E ^a,b  

^a TEXAS A AND M UNIVERSITY   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Baculovirus expression vectors; Expression systems; Glycoproteins; Glycosylation; Insect cells; Protein processing

Indexed keywords

ASPARAGINE LINKED OLIGOSACCHARIDE; GALACTOSE; GLYCOPROTEIN; RECOMBINANT PROTEIN; SIALIC ACID;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; ENZYME ACTIVITY; EXPRESSION VECTOR; GLYCOSYLATION; IMMEDIATE EARLY GENE; INSECT; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; VIRION; VIRUS RECOMBINANT;

ANIMALS; BACULOVIRIDAE; BIOTECHNOLOGY; CELL LINE; DNA, COMPLEMENTARY; GENES, IMMEDIATE-EARLY; GENETIC VECTORS; GLYCOPROTEINS; GLYCOSYLATION; N-ACETYLLACTOSAMINE SYNTHASE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SPODOPTERA; VIRAL FUSION PROTEINS;

ANIMALIA; EUKARYOTA; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0029769504     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1096-1288     Document Type: Article

References (57)

1. Summers, M.D. and Smith, G.E. 1987. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin No. 1555.
2. Jarvis, D.L. and Summers, M.D. 1992. Baculovirus expression vectors, pp. 265-291 in Recombinant DNA vaccines: rationale and strategies. Isaacson, R.E. (ed.). Marcel Dekker, Inc., New York.
3. O’Reilly, D.R., Miller, L.K., and Luckow, V.A. 1992. Baculovirus expression vectors: a laboratory manual. W.H. Freeman and Company, New York.
4. Kornfeld, R. and Kornfeld, S. 1985. Assembly of asparagine-linked oligosaccharides. Ann. Rev. Biochem. 54:631-664.
5. Jarvis, D.L. and Garcia, A., Jr. 1994. Biosynthesis and processing of the Autographa californica nuclear polyhedrosis virus gp64 protein. Virology 206:300-313.
6. B-N-acetyl-glucosamine. Biochemistry 34:2489-2495.
7. Altmann, F., Kornfeld, G., Dalik, T., Staudacher, E., and Glossl, J. 1993. Processing of asparagine-linked oligosaccharides in insect cells. N-acetylglucosaminyltransferase I and II activities in cultured lepidopteran cells. Glycobiology 3:619-625.
8. Velardo, M.A., Bretthauer, R.K., Boutaud, A., Reinhold, B., Reinhold, V.N., and Castellino, F.J. 1993. The presence of UDP-N-acetylglucosamine:α-3-D-manno-side ß-1,2-N-acetylglucosaminyl transferase I activity in Spodoptera frugiperda cells (IPLB-SF-21AE) and its enhancement as a result of baculovirus infection. J. Biol. Chem. 268:17902-17907.
9. Altmann, F. and Marz, L. 1995. Processing of asparagine-linked oligosaccharides in insect cells: evidence for α-mannosidase II. Glycoconjugate J. 12:150-155.
10. Jarvis, D.L, Bohlmeyer, D.A., Liao, Y.-F., Lomax, K., Merkle, R., Weinkauf, C., et al. 1996. Isolation and characterization of a class II α-mannosidase cDNA from lepidopteran insect cells. Glycobiology. In press.
11. Butters, T.D. and Hughes, R.C. 1981. Isolation and characterization of mosquito cell membrane glycoproteins. Biochim. Biophys. Acta 640:655-671.
12. Hsieh, P. and Robbins, P.W. 1984. Regulation of asparagine-linked oligosaccharide processing. Oligosaccharide processing in Aedes albopictus mosquito cells. J. Biol. Chem. 259:2375-2382.
13. Ryan, R.O., Anderson, D.R., Grimes, W.J., and Law, J.H. 1985. Arylphorin from Manduca sexta: carbohydrate structure and immunological studies. Arch. Biochem. Biophys. 243:115-124.
14. Nagao, E., Takahashi, N., and Chino, H. 1987. Asparagine-linked oligosaccharides of locust lipophorin. Insect Biochem. 17:531-538.
15. Kuroda, K., Geyer, H., Geyer, R., Doerfler, W., and Klenk, H.-D. 1990. The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector. Virology 174:418-429.
16. Chen, W., Shen, Q.-X., and Bahl, O.P. 1991. Carbohydrate variant of the recombinant ß-subunit of human choriogonadotropin expressed in baculovirus expression system. J. Biol. Chem. 266:4081-4087.
17. Wathen, M.W., Aeed, P.A., and Elhammer, A.P. 1991. Characterization of oligo-saccharide structures on a chimeric respiratory syncytial virus protein expressed in insect cell line Sf9. Biochemistry 30:2863-2868.
18. Williams, P.J., Wormald, M.R., Dwek, R.A., Rademacher, T.W., Parker, G.F., and Roberts, D.B. 1991. Characterization of oligosaccharides from Drosophila melanogaster glycoproteins. Biochim. Blophys. Acta 1075:146-153.
19. Knepper, T.R, Arbogast, B., Schreurs, J., and Deinzer, M.L. 1992. Determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry. Biochemistry 31:11651-11659.
20. Grabenhorst, E., Hofer, B., Nimtz, M., Jager, V, and Conradt, H.S. 1993. Biosynthesis and secretion of human inteneukin 2 glycoprotein variants from baculovirus-infected Sf21 cells. Characterization of polypeptides and posttranslational modifications. Eur. J. Biochem. 215:189-197.
21. Yeh, J.-C., Seals, J.R., Murphy, C.I., van Halbeek, H., and Cummings, R.D. 1993. Site-specific N-glycosylation and oligosaccharide structures of recombinant HIV-1 gp120 derived from a baculovirus expression system. Biochemistry 32:11087-11099.
22. Kubelka, V, Altmann, F., Kornfeld, G., and Marz, L. 1994. Structures of the N-linked oligosaccharides of the membrane glycoproteins from three lepidopteran cell lines (Sf-21, IZD-Mb-0503, Bm-N). Arch. Biochem. Biophys. 308:148-157.
23. Manneberg, M., Friedlein, A., Kurth, H., Lahm, H.-W., and Fountoulakis, M. 1994. Structural analysis and localization of the carbohydrate moieties of a soluble human interferon G receptor produced in baculovirus-infected insect cells. Protein Science 3:30-38.
24. Licari, P., Jarvis, D.L., and Bailey, J.E. 1993. Insect cell hosts for baculovirus expression vectors contain endogenous exoglycosidase activity. Biotech. Prog. 9:146-152.
25. Altmann, F., Schwihla, H., Staudacher, E., Glossl, J., and Marz, L. 1995. Insect cells contain an unusual, membrane-bound ß-N-acetylglu-cosaminidase probably involved in the processing of protein N-glycans. J. Biol. Chem. 270:17344-17349.
26. Wagner, R., Geyer, H., Geyer, R., and Klenk, H.-D. 1996. N-acetyl-ß-gluco-saminidase accounts for differences in glycosylation of influenza virus hemag-glutinin expressed in insect cells from a baculovirus vector. J. Virol. 70:4103-4109.
27. Jarvis, D.L., Weinkauf, C., and Guarino, L.A. 1996. Immediate early baculovirus vectors for foreign gene expression in transformed or infected insect cells. Protein Expression and Purification. In press.
28. Harduin-Lepers, A., Shaper, J.H., and Shaper, N.L. 1993. Characterization of two cis-regulatory regions in the murine ß-1,4-galactosyltransferase gene: evidence for a negative regulatory element that controls initiation at the proximal site. J. Biol. Chem. 268:14348-14359.
29. Russo, R.N., Shaper, N.L., Taatjes, D.J., and Shaper, J.H. 1992. Beta-1,4-galactosyltransferase: a short NH2-terminal fragment that includes the cytoplasmic and transmembrane domain is sufficient for golgi retention. J. Biol. Chem. 267:9241-9247.
30. Guarino, L.A., Gonzalez, M.A., and Summers, M.D. 1986. Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus. J. Virol. 60:224-229.
31. Rodems, S.M. and Friesen, P.D. 1993. The hr5 transcriptional enhancer stimulates early expression from the Autographa californica nuclear poly-hedrosis virus genome but is not required for virus replication. J. Virol. 67:5776-5785.
32. Smith, G.E., Fraser, M.J., and Summers, M.D. 1983. Molecular engineering of the Autographa californica nuclear polyhedrosis virus genome: deletion mutations within the polyhedrin gene. J. Virol. 46:584-593.
33. Volkman, LE. 1986. The 64K envelope protein of budded Autographa californica nuclear polyhedrosis virus. Curr. Top. Microbiol. Immunol. 131:103-118.
34. Jarvis, D.L. and Finn, E.E. 1995. Biochemical analysis of the N-glycosylation pathway in baculovirus-infected lepidopteran insect cells. Virology 212:500-511.
35. Blissard, G.W. and Wenz, J.R. 1992. Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion. J. Virol. 66:6829-6835.
36. Volkman, LE. and Goldsmith, P.A. 1985. Mechanism of neutralization of budded Autographa californica nuclear polyhedrosis virus by a monoclonal antibody: Inhibition of entry by adsorptive endocytosis. Virology 143:185-195.
37. Volkman, L.E., Goldsmith, P.A., Hess, R.T., and Faulkner, P. 1984. Neutralization of budded Autographa californica NPV by a monoclonal antibody: Identification of the target antigen. Virology 133:354-362.
38. Lee, E.U., Roth, J., and Paulson, J.C. 1989. Alteration of terminal glycosylation sequences on N-linked oligosaccharides of Chinese hamster ovary cells by expression of beta-galactoside alpha 2,6-sialyltransferase. J. Biol. Chem. 264:13848-13855.
39. Minch, S.L., Kallio, P.T., and Bailey, J.E. 1995. Tissue plasminogen activator coexpressed in Chinese hamster ovary cells with alpha(2,6)-sialyltransferase contains NeuAc alpha(2,6)Gal beta(1,4)Glc-N-AcR linkages. Biotechnol. Prog. 11:348-351.
40. Grabenhorst, E., Hoffmann, A., Nimtz, M., Zettlmeissl, G., and Conradt, H.S. 1995. Construction of stable BHK-21 cells coexpressing human secretory glyco-proteins and human Gal(ß1-4)GIcNAc-R α 2,6-sialyltransferase: α 2,6-linked NeuAc is preferentially attached to the Gal(ß1-4)GIcNAc(ß1-2)Man(α1-3)-branch of diantennary oligosaccharides from secreted recombinant beta-trace protein. Eur. J. Biochem. 232:718-725.
41. Smith, D.F., Larsen, R.D., Mattox, S., Lowe, J.B., and Cummings, R.D. 1990. Transfer and expression of a murine UDP-Gal:beta-D-Gal-alpha 1,3-galactosyl-transferase gene in transfected Chinese hamster ovary cells. Competition reactions between the alpha 1,3-galactosyltransferase and the endogenous alpha 2,3-sialyltransferase. J. Biol. Chem. 265:6225-6234.
42. Kalsner, I., Hintz, W., Reid, L.S., and Schachter, H. 1995. Insertion into Aspergillus nidulans of functional UDP-GIcNAc: alpha 3-D- mannoside beta-1,2-N-acetyl-glucosaminyl-transferase I, the enzyme catalysing the first committed step from oligomannose to hybrid and complex N-glycans. Glycoconjugate J. 12:360-370.
43. Jarvis, D.L. 1993. Continuous foreign gene expression in stably-transformed insect cells, pp. 193-217 in Insect cell culture engineering. Goosen, M.F.A., Daugulis, A., and Faulkner, P. (eds.). Marcel Dekker, Inc., New York.
44. Jarvis, D.L., Fleming, J.G.W., Kovacs, G.R., Summers, M.D., and Guarino, L.A. 1990. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably-transformed lepidopteran cells. Bio/Technology 8:950-955.
45. Jarvis, D.L. and Guarino, L.A. 1995. Continuous foreign gene expression in trans-formed lepidopteran insect cells, pp. 187-202 in Methods in molecular biology Vol 39: Baculovirus expression protocols. Richardson, C.D. (ed.). Humana Press, Clifton, N.J.
46. Wagner, R., Liedtke, S., Kretzschmar, E., Geyer, H., Geyer, R., and Klenk, H.-D. 1996. Elongation of the N-glycans of fowl plague virus hemagglutinin expressed in Spodoptera frugiperda (Sf9) cells by coexpression of human ß1,2-N-acetylglu-cosaminyltranferase I. Glycobiology 6:165-175.
47. Davidson, D.J. and Castelino, F.J. 1991. Asparagine-linked oligosaccharide processing in lepidopteran insect cells. Temporal dependence of the nature of the oligosaccharides assembted on asparagine-289 of recombinant human plasminogen produced in baculovirus vector infected Spodoptera frugiperda (IPLB-SF-21AE) cells. Biochemistry 30:6167-6174.
48. Davidson, D.J., Fraser, M.J., and Castellino, F.J. 1990. Oligosaccharide processing in the expression of human plasminogen cDNA by lepidopteran insect (Spodoptera frugiperda) cells. Biochemistry 29:5584-5590.
49. Murhammer, D.W. and Goochee, C.F. 1988. Scaleup of insect cell cultures: Protective effects of pluronic F68. Bio/Technology 6:1411-1418.
50. Kitts, P.A. and Possee, R. 1993. A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques 14:810-817.
51. Berger, E.G., Greber, U.F., and Mosbach, K. 1986. Galactosyltransferase-dependent sialylation of complex and endo-N-acetylglucosaminidase H-treated core N-glycans in vitro. FEBS Letters 203:64-68.
52. Whiteheart, S.W., Passaniti, A., Reichner, J.S., Holt, G.D., Haltiwanger, R.S., and Hart, G.W. 1989. Glycosyltransferase probes. Methods Enzymol. 179:82-95.
53. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D. et al. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
54. Hohmann, A.W. and Faulkner, P. 1983. Monoclonal antibodies to baculovirus structural proteins: determination of specificities by Western blot analysis. Virology 125: 432-444.
55. Jarvis, D.L. and Summers, M.D. 1989. Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells. Mol. Cell. Biol. 9:214-223.
56. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
57. Blake, M.S., Johnston, K.H., Russell-Jones, G.J., and Gotschlich, E.C. 1984. A rapid, sensitive method for detection of alkaline phosphatase conjugated anti-antibody on western blot. Anal. Biochem. 36:175-179.