Isolation and characterization of an insect cell line able to perform complex N-linked glycosylation on recombinant proteins

Bio/Technology

Volumn 14, Issue 2, 1996, Pages 197-202

  Ogonah, Olotu W ^a   Freedman, Robert B ^a   Jenkins, Nigel ^b   Patel, Kirit ^b   Rooney, Barrie C ^a,b  

^a UNIVERSITY OF KENT   (United Kingdom)

^b DE MONTFORT UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

RECOMBINANT GAMMA INTERFERON; RECOMBINANT PROTEIN;

ARTICLE; INSECT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN MODIFICATION; CELL LINE; GLYCOSYLATION; INSECT CELL;

INSECTA; SPODOPTERA FRUGIPERDA; UNIDENTIFIED BACULOVIRUS;

EID: 0030070842     PISSN: 0733222X     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0296-197     Document Type: Article

References (45)

1. Luckow, V. 1991. Cloning and expression of heterologous genes in insect cells with baculovirus vectors, p. 97-14. In: Recombinant DNA technology and applications. Prokop, A., Bajpai, R. K. and Ho, C. S. (Eds.). McGraw-Hill, Inc.
2. Jenkins, N. and Curling, E. M. 1994. Glycosylation of recombinant proteins: problems and prospects. Enzyme Microb. Technol. 16:354-364.
3. Butters, T. D., Hughes, R. C. and Vischer, P. 1981. Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effect of tunicamycin. Biochim. Biophys. Acta. 640:672-686.
4. Hsieh, P. and Robbins, P. W. 1984. Regulation of asparagine-Iinked processing-oligosaccharide processing in Aedes albopictus mosquito cells. J. Biol. Chem. 259:2375-2382.
5. Kretzschmar, E., Geyer, R. and Klenk H. 1994. Baculovirus infection does not alter N-glycosylation in Spodoptera frugiperda cells. J. Biol. Chem. 375:323-327.
6. Sareneva, T., Pirhonen, J., Cantell, K., Kalkkinen, N. and Julkunen, I. 1994. Role of N-glycosylation in the synthysis, dimerization and secretion of human IFN-y. Biochem. J. 303:831-840.
7. Davidson, D. J. and Castellino, F. J. 1991. Asparagine linked oligosaccharide processing in Lepidopteran insect cells. Temporal dependence of the nature of the oligosaccharides assembled on asparagine-289 of recombinant human plasminogen produced in baculovirus infected Spodoptera frugiperda (IPLBSF-2IAE) cells. Biochemistry 30:6167-6174.
8. Klenk, H. D., Cramer, A., Wagner, R., Groner, A. and Kretzschmar, E. 1992. Processing of influenza virus hemagglutinin in insect cells: variations depending on the host and on coexpression with other viral proteins, p. 166-174. Proceedings of Workship on Baculovirus and Recombinant Protein Production Processes. Interlaken, Switzerland.
9. Davis, T. R. and Wood, H. A. 1995. Intrinsic glycosylation potential of insect cell cultures and insect larvae. In Vitro Cell. Dev. Biol.-Animal 31:659-663.
10. Farrar, M. A. and Schreiber, R. D. 1993. The molecular cell biology of interferon-gamma and its receptor. Ann. Rev. Immunol. 11:571-611.
11. Curling, M., Hayter, P., Baines, A., Bull, A., Gull, K., Strange, P. and Jenkins, N. I990. Recombinant human interferon-gamma. Differences in glycosylation and proteolytic processing lead to heterogeneity in batch cultures. Biochem. J. 272:333-337.
12. Hayter, P. M., Curling, E. M. A., Baines, A. J., Jenkins, N., Salmon, I., Strange, P. G. and Bull, A. T. 1991. Chinese hamster ovary cell growth and IFN-7 production kinetics in stirred batch culture. Appl. Microbiol Biotechnol 34:559-564.
13. Hooker, A. D., Goldman, M. H., Markham, N. H., James, D. C., Ison, A. P., Bull, A. T, Strange, P. G., Salmon, I., Baines, A. J. and Jenkins, N. 1995. N-glycans of recombinant interferon-y change during batch culture of Chinese hamster ovary cells. Biotech. Bioeng., 48. In press.
14. Sutton, C., O'Neill, J. and Cottrell, J. 1994. Site specific characterisation of glycoprotein carbohydrates by exoglycosidase digestion and laser desorption mass spectrometry. Anal. Biochem. 218:34-16.
15. James, D.C., Freedman, R. B., Hoare, M., Ogonah, O., Rooney, B. C., Larinov, O. A., Dobrovolsky, V. N., Lagutin, O. V. and Jenkins, N. 1995. N-Glycosylation of recombinant human interferon-y produced in different animal expression systems. Biotechnology 13:592-596.
16. Goochee, C. F., Gramer, M. J., Andersen, D. C., Bahr, J. B. and Rasmussen, J. R. 1992. The oligosaccharides of glycoproteins: Factors affecting their synthesis and their influence on glycoprotein properties. Frontiers in Bioprocessing II, The American Chemical Society 199-230.
17. Aeed, P. A. and Elhammer, A. P. 1994. Glycosylation of recombinant prorenin in insect cells-the insect cell line Sf9 does not express the mannose-6-phosphate recognition signal. Biochemistry 33:8793-8797.
18. Castellino, F. J., Davidson, D. J., Rosen, E. and McLinden, J. 1993. Expression of human plasminogen cDNA in Lepidopteran insect cells and analysis of asparagine linked glycosylation patterns of recombinant plasminogens. Methods in Enzymology. 223:168-185.
19. Jarvis, D. L. and Finn, E. E. 1995. Biochemical-analysis of the N-glycosyla'tion pathway in baculovirus-infected lepidopteran insect cells. Virology. 212:500-511.
20. Davis, T. R., Shulcr, M. L., Granados, R. R. and Wood, H. A. 1993. Comparison of oligosaccharide processing among various insect-cell lines expressing a secreted glycoprotein. In Vitro Cell. & Dev. Biol.-Animal 29A:842-846.
21. Knepper, T. P., Arbogast, B., Schreurs, J. and Deinzer, M. L. 1992. Determination of the glycosylation patterns, disulfide linkages and protein heterogeneities of baculovirus expressed mouse interleukin-3 by mass spectometry. Biochemistry 31:11651-11659.
22. Williams, P J., Wormald, M. R., Dwek, R. A., Rademacher, T. W., Parker, G. F. and Roberts, D. R. 1991. Characterisation of oligosaccharides from Drosophila melanogaster glycoproteins. Biochim. Biophys. Acta. 1075:146-153.
23. Licari, P. J., Jams, D. L., Bailey, J. E. 1993. Insect cell hosts for baculovirus expression vectors contain endogenous exoglycosidase activity. Biotec. Prog. 9:146-152.
24. Ackermann, M, Nimtz, M., Grabenhorst, E, Conradt, H. S. and Jager, V. 1995. Pilot-scale production of glycoproteins with recombinant baculovirusesEvaluation of different host cell lines with respect to productivity, protein integrity and glycosylation. The Baculovirus and Insect Cell Gene Expression Conference. Pinehurst, NC, USA (Abstract).
25. Honda, S., Asano, T., Kajio, T., Nakagawa, S., Ikeyama, S., Ichimori, Y., Sugino, H., Nara, K., Kakinuma, A. and Kung, H. F. 1987. Differential purification by immunoaffinity chromatography of two carboxy-terminal portion deleted derivaties of recombinant interferon gamma from E coli. I. Interferon Res. 7:145-154. ,
26. Nimitz, M., Lorenz, C., Conradt, H. S. and Mohr, H. 1989. Processing of carbohydrates from signal glycoproteins secreted by human T lymphocytes: influence of induction protocol and cell differentiation state. International Symposium on Glycoconjugates 10:239 (Abstract).
27. Picard, V, Ersdalbadju, E. and Block, S. C. 1995. Partial glycosylation of antithrombin-III asparagine-135 is caused by the serine in the 3rd position of its N-glycosylation consensus sequence and is responsible for production of the beta-antithrombin-III isoform with enhanced heparin affinity. Biochemistry 34:8433-8440.
28. Davidson, D. J, Fraser, M. J. and Castellino, F. J. 1991. Alpha-mannosidase catalyzed trimming of high mannose glycans in non-infected and baculovirus infected Spodopterafrugiperda cells. Biochemistry 30:9812-9815.
29. Ren, J. X., Bretthauer, R. K. and Castellino, F. J. 1995. Purification and properties of golgi-derived (alpha-1,2) mannosidase from baculovirus infected lepidopteran insect cells (IPLB-Sf21AE) with preferential activity towards mannose.-N-acetylglucosaminej. Biochemistry 34:2489-2495.
30. Altman, F. and Marz, L. 1995. Processing of asparagine-linked oligosaccharides in insect cells-evidence for alpha mannosidase II. Glycoconjugate J. 12:150-155.
31. Altman, F., Kornfeld, G., Dalik, T., Staudacher, E. and GIössl, J. 1993. Processing of asparagine linked oligosaccharides in insect cells-N-acetylglucsaminyltransferase I and II activities in cultured lepidopteran cells. Glycobiology 3:619-625.
32. Grabenhorst, E., Hofer, B, Nimtz, M, Jager, V. and Conradt, H. S. 1993. Biosynthesis and secretion of human interIeukin-2 glycoprotein N'ariants from baculovirus infected Sf21 cells: characterization of polypeptides and post-translational modifications. Eur. J. Btochem. 215:189-197.
33. Davidson, D. J. and Castellino, F. J. 1991. Structures of the asparagine-289 linked oligosaccharides assembled on recombinant human plasminogen expressed in Mamesira brassicae cell line. Biochemistry 30:6689-6696.
34. Flesher, A. R., Marzowski, J., Wang, W. C. and Raff, H. V. 1995. Fluorophore labeled glycan analysis of immunoglobulin fusion proteins: correlation of oligosaccharide content with in vivo clearance profile. Biotec. Bioeng. 46:399-407.
35. Lodish, H. F. 1991. Recognition of complex oligosaccharides by the multi-subunit asialoglycoprotein receptor. TIBS 16:374-377.
36. Mattox, S., Walrath, K., Ceiler, D., Smith, D. F. and Cummings, R. D. 1993. A solid-phase assay for the activity of CMPNeuAc:Gal bl-4GIcNac-R oc-2,6 sialyltransferases. Anal. Biochem. 206:430-436.
37. Roth, J., Kempf, A., Reuter, G., Schauer, R. and Gehring, W. J. 1992. Occurrence of sialic acids in Drosophila melanogaster. Science 256:673-675.
38. Minch, S. L., Kallio, P. T and Bailey, J. E. 1995. Tissue plasminogen activator coexpressed in Chinese Hamster Ovary cells with alpha(2,6) sialyltransferase contain NeuAc-aIpha(2,6)-beta(l,4)GlcNAc linkages. Biotec. Prog. 11:348-351.
39. Altman, F., Treuer, V., Kubelka, V., Staudacher, E., Marz, L. and Becker, W. M. 1993. Fucose in alpha 1-3 linkage to the N-glycan core forms an allergenic epitope that occurs in plant and insect glycoproteins. Glyconjugate J. 10:310.
40. Chazenbalk, G. D. and Rapoport, B. 1995. Expression of the extracellular domain of the thyrotropin receptor in the baculovirus system using a promoter active earlier than the polyhedrin promoter-implications for the expression of functional highly glycosylated proteins, J. Biol. Chem. 270:1543-1549.
41. Jarvis, D. L. and Summers, M. D. 1989. Glycosylation and secretion of human-tissue plasminogen-activator in recombinant baculovirus-infected insect cells. Mol. Cell. Biol. 9:214-223.
42. Summers, M. D. and Smith, G. E. 1978. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agricultural Experiment Station Bulletin No. 1555.
43. Granados, R. R. and Naughton, M. 1975. Development of Amsacta moorei Entomopoxvirus virus in Ovarian and Hemocyte cultures of Estigmena acrea larvae. Intervirology 5:62-68.
44. James, D. C., Freedman, R. B., Hoare, M. and Jenkins, N. 1994. High resolution separation of recombinant human IFN-y glycoforms by micellar electrokinetic capillary chromatography. Anal. Biochemistry 222:315-322.
45. Kenney, A., Goulding, L, and Hill, C. 1988. The design, purification and uiê of immunopurification reagents, p. 99-11. In: Methods in Molecular Biology, Vol. 3, New Protein Techniques. Pulman Press Inc.