Monitoring recombinant human interferon-gamma N-glycosylation during perfused fluidized-bed and stirred-tank batch culture of CHO cells

Biotechnology and Bioengineering

Volumn 60, Issue 5, 1998, Pages 596-607

  Goldman, Merlin H ^a,b,c   James, David C ^a   Rendall, Mark ^a,b   Ison, Andrew P ^b   Hoare, Michael ^b   Bull, Alan T ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c PPL Therapeutics   (United Kingdom)

Author keywords

Capillary isoelectric focusing; Chinese hamster ovary; Glycosylation; Interferon gamma; Micellar electrokinetic capillary chromatography; Perfusion culture

Indexed keywords

BATCH CELL CULTURE; BIOREACTORS; ELECTROPHORESIS; FLUIDIZED BEDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERFERONS;

CAPILLARY ISOELECTRIC FOCUSING; GLYCOSYLATION; MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY;

GENETIC ENGINEERING;

GAMMA INTERFERON; GLYCOPROTEIN;

ANIMAL CELL; ARTICLE; BIOREACTOR; CAPILLARY CHROMATOGRAPHY; CELL DENSITY; CHO CELL; FLUIDIZED BED; GENETIC RECOMBINATION; ISOELECTRIC FOCUSING; NONHUMAN;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; ELECTROPHORESIS, CAPILLARY; GLYCOSYLATION; HUMANS; INTERFERON-GAMMA, RECOMBINANT; ISOELECTRIC FOCUSING; OLIGOSACCHARIDES; POLYSACCHARIDES;

EID: 0032488375     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19981205)60:5<596::AID-BIT10>3.0.CO;2-5     Document Type: Article

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