Stable expression of mammalian β1,4-galactosyltransferase extends the N-glycosylation pathway in insect cells

Glycobiology

Volumn 8, Issue 5, 1998, Pages 473-480

  Hollister, Jason R ^a,b   Shaper, Joel H ^c   Jarvis, Donald L ^a,b  

^a TEXAS A AND M UNIVERSITY   (United States)

^b UNIVERSITY OF WYOMING   (United States)

^c Oncology Center   (United States)

Author keywords

Baculovirus; Glycoprotein synthesis; Insect cells; Metabolic engineering; N glycosylation

Indexed keywords

GLYCOSYLTRANSFERASE; PHOSPHOTRANSFERASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL LINE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; GENE TRANSFER; GLYCOPROTEIN SYNTHESIS; IMMUNOBLOTTING; INSECT; NONHUMAN; PLASMID; PRIORITY JOURNAL;

ANIMALS; BACULOVIRIDAE; CATTLE; CELL LINE; GLYCOSYLATION; HUMANS; MAMMALS; N-ACETYLLACTOSAMINE SYNTHASE; RECOMBINANT PROTEINS; SPODOPTERA; TISSUE PLASMINOGEN ACTIVATOR; TRANSFECTION; VIRUS REPLICATION;

EID: 0031775473     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/8.5.473     Document Type: Article

References (33)

1. Altmann, F., Schwihla, H., Staudacher, E., Glössl, J. and März, L. (1995) Insect cells contain an unusual, membrane-bound β-N-acetylglucosaminidase probably involved in the processing of protein N-glycans. J. Biol. Chem., 270, 17344-17349.
2. Blake, M.S., Johnston, K.H., Russell-Jones, O.J. and Gotschlich, E.C. (1984) A rapid, sensitive method for detection of alkaline phosphatase conjugated anti-antibody on western blot. Anal. Biochem., 36, 175-179.
3. Butters, T.D., Hughes, R.C. and Vischer, P. (1981) Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin. Biochim. Biophys. Acta, 640, 672-686.
4. Davidson, D.J., Fraser, M.J. and Castellino, F.J. (1990) Oligosaccharide processing in the expression of human plasminogen cDNA by lepidopteran insect (Spodoptera frugiperda) cells. Biochemistry, 29, 5584-5590.
5. Feinberg, A.P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem., 132, 6-13.
6. Guarino, L.A. and Summers, M.D. (1987) Nucleotide sequence and temporal expression of a baculovirus regulatory gene. J. Virol., 61, 2091-2099.
7. Hirt, B. (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol., 26, 365-369.
8. Hohmann, A.W. and Faulkner, P. (1983) Monoclonal antibodies to baculovirus structural proteins: determination of specificities by Western blot analysis. Virology, 125, 432-444.
9. Jacob, G.S. and Scudder, P. (1994) Glycosidases in structural analysis. In Lennarz, W. and Hart, G. (eds.), Guide to Techniques in Glycobiology, Vol. 230. Academic Press, San Diego, pp. 280-299.
10. Jarvis, D.L. (1993) Effects of baculovirus infection on IE1-mediated foreign gene expression in stably transformed insect cells. J. Virol., 67, 2583-2591.
11. Jarvis, D.L. (1997) Baculovirus expression vectors. In Miller, L.K. (ed.), The Baculoviruses. Plenum Press, New York, pp. 389-431.
12. Jarvis, D.L. and Finn, E.E. (1995) Biochemical analysis of the N-glycosylation pathway in baculovirus-infected lepidopteran insect cells. Virology, 212, 500-511.
13. Jarvis, D.L. and Finn, E.E. (1996) Modifying the insect cell N-glycosylation pathway with immediate early baculovirus expression vectors. Nature-Biotechnology, 14, 1288-1292.
14. Jarvis, D.L. and Garcia, A., Jr. (1994) Biosynthesis and processing of the Autographa californica nuclear polyhedrosis virus gp64 protein. Virology, 205, 300-313.
15. Jarvis, D.L. and Guarino, L.A. (1995) Continuous foreign gene expression in transformed lepidopteran insect cells. In Richardson, C.D. (ed.), Baculovirus Expression Protocols, Vol. 39, Humana Press, Clifton, N.J., pp. 187-202.
16. Jarvis, D.L., Fleming, J.A., Kovacs, G.R., Summers, M.D. and Guarino, L.A. (1990) Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Bio/Technology, 8, 950-955.
17. Jarvis, D.L., Weinkauf, C. and Guarino, L.A. (1996) Immediate early baculovirus vectors for foreign gene expression in transformed or infected insect cells. Protein Expr. Pur., 8, 191-203.
18. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
19. Licari, P.J., Jarvis, D.L. and Bailey, J.E. (1993) Insect cell hosts for baculovirus expression vectors contain endogenous exoglycosidase activity. Biotech. Prog., 9, 146-152.
20. März, L., Altmann, F., Staudacher, E. and Kubelka, V. (1995) Protein glycosylation in insects. In Montreuil, J., Vliegenthart, J.F.G. and Schachter, H. (eds.), Glycoproteins, Vol. 29a, Elsevier, Amsterdam, pp. 543-563.
21. Ogonah, O.W., Freedman, R.B., Jenkins, N., Patel, K. and Rooney, B. (1996) Isolation and characterization of an insect cell line able to perform complex N-linked glycosylation on recombinant proteins. Bio/Technology, 14, 197-202.
22. O'Reilly, D.R., Miller, L.K. and Luckow, V.A. (1992) Baculovirus expression vectors. W.H. Freeman, New York.
23. Russo, R.N., Shaper, N.L. and Shaper, J.H. (1990) Bovine β1,4-galactosyltransferase: two sets of mRNA transcripts encode two forms of the protein with different amino-terminal domains. In vitro translation experiments demonstrate that both the short and the long forms of the enzyme are type II membrane-bound glycoproteins. J. Biol. Chem., 265, 3324-3331.
24. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edition. Cold Spring Harbor Press, Cold Spring Harbor, New York.
25. Shaper, N.L., Shaper, J.H., Meuth, J.L., Fox, J.L., Chang, H., Kirsch, I.R. and Hollis, G.F. (1986) Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library. Proc. Natl. Acad. Sci. USA, 83, 1573-1577.
26. Shaper, N.L., Meurer, J.A., Joziasse, D.H., Chou, T.-D.D., Smith, E.J., Schnaar, R.L. and Shaper, J.H. (1997) The chicken genome contains two functional non-allelic β1,4-galactosyltransferase genes: chromosomal assignment to syntenic regions tracks fate of the two gene lineages in the human genome. J. Biol. Chem., 272, 31389-31399.
27. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J. and Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem., 150, 76-85.
28. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 98, 503-517.
29. Summers, M.D. and Smith, G.E. (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agric. Exp. St. Bull., 1555.
30. Tarentino, A.L., Gomez, C.M. and Plummer, J.T.H. (1985) Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F. Biochemistry, 24, 4665-4671.
31. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA, 76, 4350-4354.
32. Vaughn, J.L., Goodwin, R.H., Thompkins, G.J. and McCawley, P. (1977) The establishment of two insect cell lines from the insect Spadoptera frugiperda (Lepidoptera:Noctuidae). In Vitro, 13, 213-217.
33. Waener, R., Liedtke, S., Kretzschmar, E., Geyer, H., Geyer, R. and Klenk, H.D. (1996) Elongation of the N-glycans of fowl plague virus hemagglutinin expressed in Spodoptera frugiperda (Sf9) cells by coexpression of human β1,2-N-acetylglucosaminyltranferase I. Glycobiology, 6, 165-175.