From preadipocyte to adipocyte: Differentiation-directed signals of insulin from the cell surface to the nucleus

Critical Reviews in Clinical Laboratory Sciences

Volumn 36, Issue 1, 1999, Pages 1-34

  Sorisky, Alexander ^a  

^a OTTAWA HOSPITAL RESEARCH INSTITUTE   (Canada)

Author keywords

Adipocyte differentiation; Extracellular matrix; Insulin; Insulin like growth factor 1; Obesity; p70 S6 kinase; Phosphatidylinositol 3 kinase; Preadipocyte; Protein kinase B; Ras

Indexed keywords

INSULIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; RAS PROTEIN; S6 KINASE; SOMATOMEDIN C; TRANSCRIPTION FACTOR;

ADIPOCYTE; ADIPOSE TISSUE; CARDIOVASCULAR DISEASE; CELL CULTURE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL NUCLEUS; CELL SURFACE; CONTROLLED STUDY; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; HYPERPLASIA; HYPERTROPHY; NON INSULIN DEPENDENT DIABETES MELLITUS; OBESITY; PRIORITY JOURNAL; PROADIPOCYTE; REVIEW; SIGNAL TRANSDUCTION;

ADIPOCYTES; ANIMALS; CELL DIFFERENTIATION; CELL MEMBRANE; CELL NUCLEUS; INSULIN; SIGNAL TRANSDUCTION; STEM CELLS;

EID: 0032992253     PISSN: 10408363     EISSN: None     Source Type: Journal    
DOI: 10.1080/10408369991239169     Document Type: Review

References (164)

1. 1. Sigal RJ, Warram JH. The interaction between obesity and diabetes. Curr Opin Endocrinol Diabetes 1996; 3: 3-9.
2. 2. Kuczmarski RJ, Flegal KM, Campbell SM, et al. Increasing prevalence of overweight among US adults. The National Health and Nutrition Surveys, 1960-1991. JAMA 1994; 272: 205-211.
3. 3. Macdonald SM, Reeder BA, Chen Y, et al. Obesity in Canada: a descriptive analysis. Can Med Assoc J 1991; 157: S3-S9.
4. 4. Centers for Disease Control. Daily dietary fat and total food-energy intakes: third National Health and Nutrition Examination Survey, phase 1, 1988-91. Morbid Mortal Wkly Rep 1994; 43: 116-125.
5. 5. Zachwieja JJ. Exercise as treatment for obesity. Endocrinol Metab Clin N Amer 1996; 25: 965-988.
6. 6. Manson JE, Willett WC, Stampfer MJ, et al. Body weight and mortality among women. N Engl J Med 1995; 333: 677-685.
7. 6a. Bray GA. Health hazards of obesity. Endocrinol Metab Clin N Amer 1996; 25: 907-919
8. 7. Gortmaker SL, Must A, Perrin JM, et al. Social and economic consequences of overweight in adolescence and young adulthood. N Engl J Med 1993; 329: 1008-1012.
9. 8. Rosenbaum, M, Leibel RL Hirsch J. Obesity N Engl J Med 1997; 337: 396-406.
10. 9. Bjorntorp P. "Portal" adipose tissue as a generator of risk factors for cardiovascular disease and diabetes. Arteriosclerosis 1990; 10: 493-496.
11. 10. Reaven GM. Role of insulin resistance in human disease (Syndrome X): an expanded definition. Annu Rev Medicine 1993; 44: 121-131.
12. 11. Despres JP, Moorjani S, Lupien PJ, et al. Regional distribution of body fat, plasma lipoproteins, and cardiovascular disease. Arteriosclerosis 1990; 10: 497-511.
13. 12. Reaven GM, Lithell H Landsberg L. Hypertension and associated metabolic abnormalities-the role of insulin resistance and the sympathoadrenal system. N Engl J Med 1996; 334: 374-381.
14. 13. Bouchard C, Despres J-P Mauriege P. Genetic and nongenetic determinants of regional fat distribution. Endocrinol Rev 1993; 14: 72-93.
15. 14. Despres JP, Lamarche B, Mauriege P, et al. Hyperinsulinemia as an independent risk factor for ischemic heart disease. N Engl J Med 1996; 334: 952-957.
16. 15. Hsueh W, Law RE, Saad M, et al. Insulin resistance and macrovascular disease. Curr Opin Endocrinol Diabetes 1996; 3: 346-354.
17. 16. Hotamisligil GS, Shargill NS Spiegelman BM. Adipose expression of tumor necrosis factor-α: direct role in obesity-linked insulin resistance. Science 1993; 259: 87-91.
18. 17. Hotamisligil GS, Arner P, Caro JF, et al. Increased adipose tissue expression of tumor necrosis factor-α in human obesity and insulin resistance. J Clin Invest 1995; 95: 2409-2415.
19. 18. Zhang Y, Proenca R, Maffel M, et al. Positional cloning of the mouse obese gene and its human homologue. Nature 1994; 372: 425-432.
20. 19. Shimabukuro M, Koyama K, Chen G, et al. Direct antidiabetic effect of leptin through triglyceride depletion of tissues. Proc Natl Acad Sci USA 1997; 94: 4637-4641.
21. 20. Shen GX, Angel A. Regulation of cholesterol ester transfer activity in adipose tissue: comparison between hamster and rat species. Am J Physiol 1995; 269: E99-107.
22. 21. Radeau T, Lau P, Robb M, et al. Cholesterol ester transfer protein (CETP) mRNA abundance in human adipose tissue relationship to cell size and membrane cholesterol content. J Lipid Res 1995; 36: 2552-2561.
23. 22. Frederich RC Jr, Kahn BB, Peach MJ et al. Tissue-specific nutritional regulation of angiotensinogen in adipose tissue. Hypertension 1992; 19: 339-344.
24. 23. Hu E, Liang P Spiegelman BM. AdipoQ is a novel adipose-specific gene dysregulated in obesity. J Biol Chem 1996; 271: 10697-10703.
25. 24. Rosen BS, Cook KS, Yaglom J, et al. Adipsin and complement factor D activity: an immune-related defect in obesity. Science 1989; 244: 1483-1487.
26. 25. Scherer PE, Williams S, Fogliano M, et al. A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem 1995; 270: 26746-26749.
27. 26. Baldo A, Sniderman AD, St. Luce S, et al. The adipsin/acylation stimulating protein system and regulation of intracellular triglyceride synthesis. J Clin Invest 1993; 92: 1543-1547.
28. 27. Shimomura I, Funahashi T, Takahashi M, et al. Enhanced expression of PAI-1 in visceral fat: possible contributor to vascular disease in obesity. Nature Medicine 1996; 2: 800-803.
29. 28. Juhan-Vague I, Alessi MC Vague P. Increased plasminogen activator inhibitor I levels: a possible link between insulin resistance and atherothrombosis. Diabetologia 1991; 34: 457-462.
30. 29. Curfman GD. Diet pills redux. N Engl J Med 1997; 337: 629-630.
31. 30. NIH Health Technology Assessment Conference Panel. Methods for voluntary weight loss and control. Ann Intern Med 1992; 116: 942-949.
32. 31. Eckel RH. Lipoprotein lipase. N Engl J Med 1989; 320: 1060-1068.
33. 32. Eckel RH, Yost TJ. Weight reduction increases adipose tissue lipoprotein lipase responsiveness in obese women. J Clin Invest 1987; 80: 992-997.
34. 33. Schwartz R, Brunzell JD. Increase of adipose tissue lipoprotein lipase activity with weight loss. J Clin Invest 1981; 67: 1425.
35. 34. Hirsch J, Fried SK, Edens NK, et al. The fat cell. Med Clin N Am 1989; 73: 83-96.
36. 35. Bougneres P, Le Stunff C, Pecqueur C, et al. In vivo resistance of lipolysis to epinephrine. J Clin Invest. 1997; 99: 2568-2573.
37. 36. Ailhaud G, Grimaldi P, Negrel R. Hormonal regulation of adipose differentiation. Trends Endocrinol Metab 1994; 5: 132-136.
38. 37. Shillabeer G, Forden JM, Lau DCW. Induction of preadipocyte differentiation by mature fat cells in the rat. J Clin Invest 1989; 84: 381-387.
39. 38. Kim JB, Sarraf P, Wright M, et al. Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1. J Clin Invest 1998; 101: 1-9.
40. 39. Brun RP, Kim JB, Hu E, et al. Adipocyte differentiation: a transcriptional regulatory cascade. Curr Opin Cell Biol 1996; 8: 826-832.
41. 39a. Amri EZ, Bertrand B, Ailhaud G, et al. Regulation of adipose cell differentiation. I. Fatty acids are inducers of the aP2 gene expression. J Lipid Res 1991, 32: 1449-1456.
42. 40. Klaus S. Functional differentiation of white and brown adipocytes. BioEssays 1997; 19: 215-223.
43. 41. Cornelius P, MacDougald OA, Lane MD. Regulation of adipocyte development. Annu Rev Nutr 1994; 14: 99-129.
44. 42. Dani C, Smith AG, Dessolin S, et al. Differentiation of embryonic stem cells into adipocytes in vitro. J Cell Sci 1997; 110: 1279-1285.
45. 43. Ailhaud G, Grimaldi P, Negrel R. Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr 1992; 12: 207-233.
46. 44. Smas CM, Sul HS. Control of adipocyte differentiation. Biochem J 1995; 309: 697-710.
47. 45. MacDougald OA, Lane MD. Transcriptional regulation of gene expression during adipocyte differentiation. Annu Rev Biochem 1995; 64: 345-373.
48. 46. Lemonnier D. Effect of age, sex, and site on the cellularity of the adipose tissue in mice and rats rendered obese by a high-fat diet. J Clin Invest 1972; 51: 2907-2912.
49. 47. Faust IM, Johnson PR, Stern JS, et al. Diet-induced adipocyte number increase in adult rats: a new model of obesity. Am J Physiol 1978; 235: E279-E286.
50. 48. Ochi M, Yoshioka H, Sawada T, et al. New adipocyte formation in mice during refeeding after long-term deprivation. Am J Physiol 1991; 260: R468-R474.
51. 49. Shepherd PR, Gnudi L, Tozzo E, et al. Adipose cell hyperplasia and enhanced glucose disposal in transgenic mice overexpressing GLUT4 selectively in adipose tissue. J Biol Chem 1993; 268: 22243-22246.
52. 50. Hirsch J, Batchelor B. Adipose tissue cellularity in human obesity. Clin Endocrinol Metab 1976; 5: 299-311.
53. 51. Poznanski WJ, Waheed I, Van R. Human fat cell precursors. Laboratory Invest 1973; 29: 570-576.
54. 52. Van RLR, Bayliss CE, Roncari DAK. Cytological and enzymological characterization of adult human adipocyte precursors in culture. J Clin Invest 1976; 58: 699-704.
55. 53. Hauner H, Entenmann G, Wabitsch M, et al. Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium. J Clin Invest 1989; 84: 1663-1670.
56. 54. Roncari DAK. Pre-adipose cell replication and differentiation. Trends Biochem Sci 1984; 9: 486-489.
57. 55. Sugihara H, Funatsumaru S, Yonemitzu N, et al. A simple culture method of fat cells from mature fat tissue fragments. J Lipid Res 1989; 30: 1987-1995.
58. 56. Carraro R, Li ZH, Johnson JE Jr, et al. Islets of preadipocytes highly committed to differentiation in cultures of adherent rat adipocytes. Cell Tissue Res 1991; 264: 243-251.
59. 57. Entenmann G, Hauner H. Relationship between replication and differentiation in cultured human adipocyte precursor cells. Am J Physiol 1996; 270: C1011-C1016.
60. 58. Green H, Kehinde O. Sublines of mouse 3T3 cells that accumulate lipid. Cell 1974; 1: 113-116.
61. 59. Novikoff AB, Novikoff PM, Rosen OM. Organelle relationships in cultured 3T3-L1 preadipocytes. J Cell Biol 1980; 87: 180-196.
62. 60. Green H, Kehinde O. Formation of normally differentiated subcutaneous fat pads by an established preadipose cell line. J Cell Physiol 1979; 101: 169-172.
63. 61. Mandrup S, Loftus TM, MacDougald OA, et al. Obese gene expression at in vitro levels by fat pads derived from s.c. implanted 3T3-F442A preadipocytes. Proc Natl Acad Sci USA 1997; 94: 4300-4305.
64. 61a. Gaillard D, Poli P, Negrel R. Characterization of ouabain-resistant mutants of the preadipocyte Ob17 clonal cell line. Exp Cell Res 1985, 156: 513-527.
65. 62. Mandrup S, Lane MD. Regulating adipogenesis. J Biol Chem 1997; 272: 5367-5370.
66. 63. Shao D, Lazar MA. Peroxisome proliferator activated receptor γ, CCAT/Enhancer-binding protein α, and cell cycle status regulate the commitment to adipocyte differentiation. J Biol Chem 1997; 272: 21473-21478.
67. 64. Lin FT, Lane MD. CCAAT/enhancer binding protein a is sufficient to initiate the 3T3-L1 adipocyte differentiation program. Proc Natl Acad Sci USA 1994; 91: 8757-8761.
68. 65. Wang N, Finegold MJ, Bradley A, et al. Impaired energy homeostasis in C/EBPα knockout mice. Science 1995; 269: 1108-1112.
69. 66. Yeh WC, McKnight SL. Regulation of adipose maturation and energy homeostasis. Curr Opin Cell Biol 1995; 7: 885-890.
70. 67. Wu Z, Bucher NLR, Farmer SR. Induction of peroxisome proliferator-activated receptor g during the conversion of 3T3 fibroblasts into adipocytes is mediated by C/EBPb, C/EBPd, and glucocorticoids. Mol Cell Biol 1996; 16: 4128-4136.
71. 68. Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblasts by PPARg2, a lipid-activated transcription factor. Cell 1994; 79: 1147-1156.
72. 69. Kliewer SA, Lenhard JM, Willson TM, et al. A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor γ and promotes adipocyte differentiation. Cell 1995; 83: 813-819.
73. 70. Forman BM, Tontonoz P, Chen J, et al. 15-deoxy-D12,14-prostaglandin J2 is a ligand for the adipocyte determination factor PPARγ. Cell 1995; 83: 803-812.
74. 71. Lehmann JM, Moore LB, Smith-Oliver TA, et al. An antidiabetic thiazolinedione is a high affinity ligand for peroxisome proliferator-activated receptor γ (PPARγ). J Biol Chem 1995; 270: 12953-12956.
75. 72. Kletzien RF, Clark SD, Ulrich RG. Enhancement of adipocyte differentiation by an insulin-sensitizing agent. Mol Pharmacol 1992; 41: 393-398.
76. 73. Reginato MJ, Krakow SL, Bailey ST, et al. Prostaglandins promote and block adipogenesis through opposing effects on peroxisome proliferator-activated receptor γ. J Biol Chem 1997; 273: 1855-1858.
77. 74. Nolan JJ, Ludvik B, Beerdsen P, et al. Improvement in glucose tolerance and insulin resistance in obese subjects treated with troglitazone. N Engl J Med 1994; 331: 1188-1193.
78. 75. Smith PJ, Wise LS, Berkowitz R, et al. Insulin-like growth factor-1 is an essential regulator of the differentiation of 3T3-L1 adipocytes. J Biol Chem 1988; 263: 9402-9408.
79. 76. Cheatham B, Kahn CR. Insulin action and the insulin signaling network. Endocrinol Rev 1995; 16: 117-142.
80. 77. Cohick WS, Clemmons DR. The insulin-like growth factors. Annu Rev Physiol 1993; 55: 131-153.
81. 78. Rubin CS, Hirsch A, Fung C, et al. Development of hormone receptors and hormonal responsiveness in vitro. J Biol Chem 1978; 253: 7570-7578.
82. 79. Accili D, Taylor SI. Targeted inactivation of the insulin receptor gene in mouse 3T3-L1 fibroblasts via homologous recombination. Proc Natl Acad Sci USA 1991; 88: 4708-4712.
83. 80. Chaika OV, Chaika N, Volle DJ, et al. CSF-1 receptor/insulin receptor chimera permits CSF-1-dependent differentiation of 3T3-L1 preadipocytes. J Biol Chem 1997; 272: 11968-11974.
84. 80a. Gagnon AM, Sorisky A. The effect of glucose concentration on insulin-induced 3T3-L1 adipose cell differentiation. Obesity Res 1998; in press.
85. 80b. Hauner H. Complete adipose differentiation of 3T3-L1 cells in a chemically defined medium: comparison to serum-containing culture conditions. Endocrinology 1990; 127: 865-872.
86. 81. Olefsky JM. Insulin resistance in non-insulin-dependent diabetes mellitus. Curr Opin Endocrinol Diabetes 1995; 2: 290-299.
87. 82. Schubert C, Carel K, DePaola D, et al. Interactions of protein kinase C with insulin signaling. J Biol Chem 1996; 271: 15311-15314.
88. 83. American Type Culture Collection, 1993; Product Sheet CCL 92.1.
89. 84. Myers MG Jr, White MF. New frontiers in insulin receptor substrate signaling. Trends Endocrinol Metab 1995; 6: 209-215.
90. 85. Pawson T, Scott JD. Signaling through scaffold, anchoring, and adaptor proteins. Science 1997; 278: 2075-2080.
91. 86. Sun XJ, Wang LM, Zhang Y, et al. Role of IRS-2 in insulin and cytokine signaling. Nature 1995; 377: 173-177.
92. 87. Sun XJ, Pons S, Wang LM, et al. The IRS-2 gene on murine chromosome 8 encodes a unique signaling adapter for insulin and cytokine action. Mol Endocrinol 1997; 11: 251-262.
93. 88. Lavan BE, Lane WS, Lienhard GE. The 60-kDa phosphotyrosine protein in insulin-treated adipocytes is a new member of the insulin receptor substrate family. J Biol Chem 1997; 272: 11439-11443.
94. 89. Lavan BE, Fantin VR, Chang ET, et al. A novel 160-kDa phosphotyrosine protein in insulin-treated embryonic kidney cells is a new member of the insulin receptor substrate family. J Biol Chem 1997; 272: 21403-21407.
95. 90. Saad MJA, Folli F, Araki E, et al. Regulation of insulin receptor, insulin receptor substrate-1 and phosphatidylinositol 3-kinase in 3T3-F442A adipocytes. Effects of differentiation, insulin, and dexamethasone. Mol Endocrinol 1994; 8: 545-557.
96. 91. Hu Q, Klippel A, Muslin AJ, et al. Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol 3-kinase. Science 1995; 268: 100-102.
97. 92. Rodriguez-Viciana P, Warne PH, Dhand R. Phosphatidylinositol-3-OH kinase as a direct target of Ras. Nature 1994; 370: 527-532.
98. 93. Hall A. Ras-related proteins. Curr Opin Cell Biol 1993; 5: 265-268.
99. 94. Egan SE, Weinberg RA. The pathway to signal achievement. Nature 1993; 365: 781-783.
100. 95. Bar-Sagi D. The Sos (Son of sevenless) protein. Trends Endocrinol Metab 1994; 5: 165-169.
101. 96. Cantley LC, Auger KR, Carpenter C, et al. Oncogenes and signal transduction. Cell 1991; 64: 281-302.
102. 97. Gibbs JB, Oliff A Kohl NE. Farnesyltransferase inhibitors: Ras research yields a potential cancer therapeutic. Cell 1994; 77: 175-178.
103. 98. Hall A. A biochemical function for Ras - at last. Science 1994; 264: 1413-1414.
104. 99. Benito M, Porras A, Nebreda AR, et al. Differentiation of 3T3-L1 fibroblasts to adipocytes induced by transfection of ras oncogenes. Science 1991; 253: 565-568.
105. 100. Porras A, Nebreda AR, Benito M, et al. Activation of Ras by insulin in 3T3-L1 cells does not involve GTPase-activating protein phosphorylation. J Biol Chem 1992; 267: 21124-21131.
106. 101. Porras A, Muszynski K., Rapp UR, et al. Dissociation between activation of Raf-1 kinase and the 42-kDa mitogen-activated protein kinase/90-kDa S6 kinase (MAPK/RSK) cascade in the insulin/Ras pathway of adipocytic differentiation of 3T3 L1 cells. J Biol Chem 1994; 269: 12741-12748.
107. 102. Demora J, Porras A, Ahn N, et al. Mitogen-activated protein kinase activation is not necesaary for, but antagonizes, 3T3-L1 adipocytic differentiation. Mol Cell Biol 1997; 17: 6068-6075.
108. 103. Hu E, Kim JB, Sarraf P, et al. Inhibition of adipogenesis through MAP kinase-mediated phosphorylation of PPARγ. Science 1996; 274: 2100-2103.
109. 104. Sale EM, Atkinson PGP Sale GJ. Requirement of MAP kinase for differentiation of fibroblasts to adipocytes, for insulin activation of p90 S6 kinase and for insulin or serum stimulation of DNA synthesis. EMBO J 1995; 14: 674-684.
110. 105. Tomiyama K, Nakata, H, Sasa H, et al. Wortmannin, a specific phosphatidylinositol 3-kinase inhibitor, inhibits adipocytic differentiation of 3T3-L1 cells. Biochem Biophys Res Commun 1995; 212: 263-269.
111. 106. Magun R, Burgering BMT, Coffer P, et al. Expression of a constitutively activated form of protein kinase B (c-Akt) in 3T3-L1 preadipose cells causes spontaneous differentiation. Endocrinology 1996; 137: 3590-3593.
112. 107. Carpenter CL, Cantley LC. Phosphoinositide kinases. Curr Opin Cell Biol 1996; 8: 153-158.
113. 108. Stephens LR, Hughes KT, Irvine RF. Pathway of phosphatidylinositol(3,4,5)-trisphosphate synthesis in activated neutrophils. Nature 1991; 351: 33-39.
114. 109. Hawkins PT, Jackson TR Stephens LR. Platelet-derived growth factor stimulates synthesis of PtdIns(3,4,5)P3 by activating a PtdIns(4,5)P2 3-OH kinase. Nature 1992; 358: 157-159.
115. 110. Irvine RF. Inositol lipids in cell signaling. Curr Opin Cell Biology 1992; 4: 212-219.
116. 111. Serunian LA, Haber MT, Fukui T, et al. Polyphosphoinositides produced by phosphatidylinositol 3-kinase are poor substrates for phospholipase C from rat liver and bovine brain. J Biol Chem 1989; 264: 17809-17815.
117. 112. Toker A, Cantley LC. Signaling through the lipid products of phosphoinositide-3-OH kinase. Nature 1997; 387: 673-676.
118. 113. Kapeller R, Cantley LC. Phosphatidylinositol 3-kinase. BioEssays 1994; 16: 565-576.
119. 114. Dhand R, Hara K, Hiles I, et al. PI 3-kinase: structural and functional analysis of intersubunit interactions. EMBO J 1994; 13: 511-521.
120. 115. Vanhaesebroeck B, Leevers SJ, Panayoyou G, et al. Phosphoinositide 3-kinases: a conserved family of signal transducers. Trends Biochem Sci 1997; 22: 267-272.
121. 116. Guinebault C, Payrastre B, Racaud-Sultan C, et al. Integrin-dependent translocation of phosphoinositide 3-kinase to the cytoskeleton of thrombin-activated platelets involves specific interactions of p85a with actin filaments and focal adhesion kinase. J Cell Biol 1995; 129: 831-842.
122. 117. Hunter T. When is a lipid kinase not a lipid kinase? When it is a protein kinase. Cell 1995; 83: 1-4.
123. 118. Dhand R, Hiles I, Panayotou G, et al. PI 3-kinase is a dual specificity enzyme: autoregulation by an intrinsic protein-serine kinase activity. EMBO J 1994; 13: 522-533.
124. 119. Lam K, Carpenter CL, Ruderman NB, et al. J Biol Chem 1994; 269: 20648-20652.
125. 120. Sorisky A, Pardasani D Lin Y. The 3-phosphorylated phosphoinositide response of 3T3-L1 preadipose cells exposed to insulin, insulin-like growth factor-1, or platelet-derived growth factor. Obesity Res 1996; 4: 9-19.
126. 121. Ui M, Okada T, Hazeki K, et al. Wortmannin as a unique probe for an intracellular signaling protein, phosphoinositide 3-kinase. Trends Biochem Sci 1995; 20: 303-307.
127. 122. Chou MM, Blenis J. The 70 kDa S6 kinase: regulation of a kinase with multiple roles in mitogenic signaling. Curr Opin Cell Biol 1995; 7: 806-814.
128. 123. Yeh WC, Bierer BE, McKnight SL. Rapamycin inhibits clonal expansion and adipogenic differentiation of 3T3-L1 cells. Proc Natl Acad Sci USA 1995; 92: 11086-11090.
129. 124. Downward J. Lipid-regulated kinases: some common themes at last. Science 1998; 279: 673-674.
130. 125. Abel ED, Houseknecht KL, Kahn BB. Recent advances in understanding the molecular basis for insulin-stimulated glucose transport. Curr Opin Endocrinol Diabetes 1995; 2: 313-324.
131. 126. Birnboim MJ, James DE. The insulin-regulatable glucose transporter GLUT-4. Curr Opin Endocrinol Diabetes 1995; 2: 383-391.
132. 127. Coffer PJ, Woodgett JR. Molecular cloning and characterization of a novel putative protein-serine kinase related to the cAMP-dependent and protein kinase C families. Eur J Biochem 1991; 201: 475-481.
133. 128. Bellacosa A, Testa JR, Staal SP, et al. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like domain. Science 1991; 254: 274-277.
134. 129. Jones PF, Jakubowicz T, Pitossi FJ, et al. Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc Natl Acad Sci USA 1991; 88: 4171-4175.
135. 130. Andjelkovic M, Alessi DR, Meier R, et al. Role of translocation in the activation and function of protein kinase B. J Biol Chem 1997; 272: 31515-31524.
136. 131. Marte BM, Downward J. PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond. Trends Biochem Sci 1997; 22: 355-358.
137. 132. Burgering BMT, Coffer P.J. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 1995; 376: 599-602.
138. 133. Franke TF, Yang SI, Chan TO, et al. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 1995; 81: 727-736.
139. 134. Kohn AD, Kovacina KS Roth, RA. Insulin stimulates the kinase activity of RAC-PK, a pleckstrin homology domain containing ser/thr kinase. EMBO J 1995; 14: 4288-4295.
140. 135. Konishi H, Kuroda S, Kikkawa, U. The pleckstrin homology domain of Rac protein kinase associates with the regulatory domain of protein kinase Cz. Biochem Biophys Res Commun 1994; 205: 1770-1775.
141. 136. Konishi H, Matsuzaki H, Tanaka M, et al. Proc Natl Acad Sci USA 1996; 93: 7639-7643.
142. 137. Stokoe D, Stephens LR, Copeland T, et al. Dual role of phosphatidylinositol-3,4,5-trisphosphate in the activation of protein kinase B. Science 1997; 277: 567-570.
143. 138. Klippel A, Kavanaugh WM, Pot D, et al. A specific product of phosphatidylinositol 3-kinase directly activates the protein kinase Akt through its pleckstrin homology domain. Mol Cell Biol 1996; 17: 338-344.
144. 139. Stephens L, Anderson K, Stokoe D, et al. Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B. Science 1998; 279: 710-714.
145. 140. Kohn AD, Summers SA, Birnbaum MJ, et al. Expression of a constitutively active Akt ser/thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation. J Biol Chem 1996; 271: 31372-31378.
146. 141. Pullen N, Dennis PB, Andjelkovic M, et al. Phosphorylation and activation of p70sK by PDK1. Science 1998; 279: 707-710.
147. 142. Cross DAE., Alessi DR, Cohen P, et al. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 1995; 378: 785-789.
148. 143. Franke TF, Cantley LC. A Bad kinase makes good. Nature 1997; 390: 116-117.
149. 144. Dudek H, Datta SR, Franke TF, et al. Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 1997; 275: 661-665.
150. 145. Wijkander J, Landstrom TR, Manganiello V, et al. Insulin-induced phosphorylation and activation of phosphodiesterase 3B in rat adipocytes: possible role for protein kinase B but not mitogen-activated protein kinase or p70 S6 kinase. Endocrinology 1998; 139: 219-227.
151. 146. Benjamin WB, Pentyala SN, Woodgett JR, et al. ATP citrate-lyase and glycogen synthase kinase-3b in 3T3-L1 cells during differentiation into adipocytes. Biochem J 1994; 1994: 477-482.
152. 147. de Groot RP, Ballou LM Sassone-Corsi P. Positive regulation of the cAMP-responsive activator CREM by the p70 S6 kinase. Cell 1994; 79: 81-91.
153. 148. Downward J. Regulating S6 kinase. Nature 1994; 371: 378-379.
154. 149. de Groot RP, Auwerx J Sassone-Corsi P. Negative regulation of Jun/AP-1. Oncogene 1993; 8: 841-847.
155. 150. Kim SJ, Kahn CR. Insulin stimulates phosphorylation of c-Jun, c-Fos and Fos-related proteins in cultured adipocytes. J Biol Chem 1994; 269: 11887-11892.
156. 151. Spiegelman BM, Farmer SR. Decreases in tubulin and actin gene expression prior to morphological differentiation of 3T3 adipocytes. Cell 1982; 29: 53-60.
157. 152. Kuri-Harcuch W, Wise LS Green H. Interruption of the adipose conversion of 3T3 cells by biotin deficiency: differentiation without triglyceride accumulation. Cell 1978; 14: 53-59.
158. 153. Spiegelman BM, Ginty CA. Fibronectin modulation of cell shape and lipogenic gene expression in 3T3-adipocytes. Cell 1983; 35: 657-666.
159. 154. Franke WW, Hergt M Grund C. Rearrangement of the vimentin cytoskeleton during adipose conversion: formation of an intermediate filament cage around lipid globules. Cell 1987; 49: 131-141.
160. 155. Clark EA, Brugge JS. Integrins and signal transduction pathways: the road taken. Science 1995; 268: 233-239.
161. 156. Ignotz RA, Massague J. Type b transforming growth factor controls the adipogenic differentiation of 3T3 fibroblasts. Proc Natl Acad Sci USA 1985; 82: 8530-8534.
162. 157. Ignotz RA, Massague J. Transforming growth factor-b stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix. J Biol Chem 1986; 261: 4337-4345.
163. 158. Smas CM, Sui HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993; 73: 725-734.
164. 159. Gagnon AM, Chabot J., Pardasani D, et al. Extracellular matrix induced by TGFβ impairs insulin signal transduction in 3T3-L1 preadipose cells. J Cell Physiol 1998; in press.