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Mol Cell Biol
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Lodish, H.1
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Mitchison TJ, Kirschner M. Dynamic instability of microtubule growth. Nature. 312:1984;237-242.
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Margolis RL, Wilson L. Microtuble treadmills-possible molecular machinery. Nature. 293:1981;705-711.
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Nature
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Margolis, R.L.1
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0031013546
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of special interest. This paper contains an elegant demonstration of rapid microtubule treadmilling in melanophore cell cytoplasts preparations in the absence of delectable dynamic instability behavior.
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Rodionov VI, Borisy GG. Microtubule treadmilling in vivo. of special interest Science. 275:1997;215-218 This paper contains an elegant demonstration of rapid microtubule treadmilling in melanophore cell cytoplasts preparations in the absence of delectable dynamic instability behavior.
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Science
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Rodionov, V.I.1
Borisy, G.G.2
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6
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0031172760
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Microtubule dynamics: Treadmilling comes around again
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of special interest. This paper summarizes recent data on microtubule treadmilling in living cells
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Waterman-Storer CM, Salmon ED. Microtubule dynamics: treadmilling comes around again. of special interest Curr Biol. 7:1997;369-372 This paper summarizes recent data on microtubule treadmilling in living cells.
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Curr Biol
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Waterman-Storer, C.M.1
Salmon, E.D.2
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Exchange of actin subunits in the leading edge of living fibroblasts: Possible of treadmilling
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Wang Y-L. Exchange of actin subunits in the leading edge of living fibroblasts: possible of treadmilling. J Cell Biol. 101:1985;597-602.
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Wang Y-L1
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Theriot JA, Mitchison TJ. Actin microfilament dynamics in locomoting cells. Nature. 352:1991;126-131.
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Nature
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Theriot, J.A.1
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0025153023
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Kinetochores capture astral microtubules during chromosomes attachment to the mitotic spindle: Direct visualization in live newt cells
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Hayden JJ, Bowser SS, Rieder C. Kinetochores capture astral microtubules during chromosomes attachment to the mitotic spindle: direct visualization in live newt cells. J Cell Biol. 111:1990;1039-1045.
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Hayden, J.J.1
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10
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0025098191
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Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle
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Rieder CL, Alexander SP, Rupp G. Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle. J Cell Biol. 110:1990;81-95.
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J Cell Biol
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Rieder, C.L.1
Alexander, S.P.2
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0029360519
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Microtubule dynamics: Taking aim at a moving target
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Wilson L, Jordan MA. Microtubule dynamics: taking aim at a moving target. Chem Biol. 2:1995;569-573.
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(1995)
Chem Biol
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, pp. 569-573
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Wilson, L.1
Jordan, M.A.2
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12
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0030798735
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Accelerating on a treadmill: ADF/cofilin promotes rapid actin filament turnover in the dynamic cytoskeleton
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of outstanding interest. This paper describes, in summary form, the results of recent papers showing that the dynamics of actin filaments are much more rapid in cells than in vitro, and that the rapid dynamics are promoted by a group of small actin binding proteins including cofilin, destrin, depactin, actophorin, and actin depolymerizing factor (ADF).
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Theriot JA. Accelerating on a treadmill: ADF/cofilin promotes rapid actin filament turnover in the dynamic cytoskeleton. of outstanding interest J Cell Biol. 136:1997;1165-1168 This paper describes, in summary form, the results of recent papers showing that the dynamics of actin filaments are much more rapid in cells than in vitro, and that the rapid dynamics are promoted by a group of small actin binding proteins including cofilin, destrin, depactin, actophorin, and actin depolymerizing factor (ADF).
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(1997)
J Cell Biol
, vol.136
, pp. 1165-1168
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Theriot, J.A.1
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13
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0000297058
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Taxol stabilizes microtubules in mouse fibroblasts cells
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Schiff PB, Horwitz SB. Taxol stabilizes microtubules in mouse fibroblasts cells. Proc Natl Acad Sci USA. 77:1980;1561-1565.
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(1980)
Proc Natl Acad Sci USA
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, pp. 1561-1565
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Schiff, P.B.1
Horwitz, S.B.2
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14
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0026425674
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Experimental antitumor activity of taxotere (RP 56976, NSC 628503), a taxol analogue
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Bissery M-C, Guenard D, Gueritte-Voegelein F, Lavelle F. Experimental antitumor activity of taxotere (RP 56976, NSC 628503), a taxol analogue. Cancer Res. 51:1991;4845-4852.
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Cancer Res
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Bissery M-C1
Guenard, D.2
Gueritte-Voegelein, F.3
Lavelle, F.4
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15
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0030113284
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(+)-Discodermolide binds to microtubules in stoichiometric ratio to tubulin dimers, blocks taxol binding and results in mitotic arrest
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of special interest. Here, the authors report that discodermolide competes with taxol for binding to microtubules and binds with higher affinity than taxol. It also induces M phase arrest and bundles microtubles in cells.
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Hung DT, Chen J, Schrieber SL. (+)-Discodermolide binds to microtubules in stoichiometric ratio to tubulin dimers, blocks taxol binding and results in mitotic arrest. of special interest Chem Biol. 3:1996;287-293 Here, the authors report that discodermolide competes with taxol for binding to microtubules and binds with higher affinity than taxol. It also induces M phase arrest and bundles microtubles in cells.
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(1996)
Chem Biol
, vol.3
, pp. 287-293
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Hung, D.T.1
Chen, J.2
Schrieber, S.L.3
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16
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0030039669
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Discodermolide, a cytotoxic marine agent that stabilizes microtubules more potently than taxol
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of special interest. In the paper it is shown that discodermolide is a cytotoxic marine agent that induces mitotic arrest in Burkitt lymphoma cells, bundles microtubules in cells, and is more potent than taxol in inducing tubulin to form microtubules at low temperature, in the absence of GTP and/or microtubule binding proteins.
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Ter Haar E, Kowalski R, Hamel E, Lin C, Longley R, Gunasekera S, Rosenkranz H, Day BW. Discodermolide, a cytotoxic marine agent that stabilizes microtubules more potently than taxol. of special interest Biochemistry. 35:1996;243-250 In the paper it is shown that discodermolide is a cytotoxic marine agent that induces mitotic arrest in Burkitt lymphoma cells, bundles microtubules in cells, and is more potent than taxol in inducing tubulin to form microtubules at low temperature, in the absence of GTP and/or microtubule binding proteins.
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(1996)
Biochemistry
, vol.35
, pp. 243-250
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Ter Haar, E.1
Kowalski, R.2
Hamel, E.3
Lin, C.4
Longley, R.5
Gunasekera, S.6
Rosenkranz, H.7
Day, B.W.8
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17
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0031027531
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Activities of the microtubule-stabilizing agents epothilones A and B with purified tubulin and in cells resistant to paclitaxel (Taxol(R))
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of special interest. This paper shows that epothilones A and B block mitosis, induce tubulin to form microtubules at low temperature in the absence of GTP and/or microtubule binding proteins, and competitively inhibit the binding of taxol to tubulin polymers. Epothilone B is shown to be more potent in cells than epothilone A or taxol. Drug-resistant colon and ovarian cell lines retain sensitivity to the epothilones.
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Kowalski R, Giannakakou P, Hamel E. Activities of the microtubule-stabilizing agents epothilones A and B with purified tubulin and in cells resistant to paclitaxel (Taxol(R)). of special interest J Biol Chem. 272:1997;2534-2541 This paper shows that epothilones A and B block mitosis, induce tubulin to form microtubules at low temperature in the absence of GTP and/or microtubule binding proteins, and competitively inhibit the binding of taxol to tubulin polymers. Epothilone B is shown to be more potent in cells than epothilone A or taxol. Drug-resistant colon and ovarian cell lines retain sensitivity to the epothilones.
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(1997)
J Biol Chem
, vol.272
, pp. 2534-2541
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Kowalski, R.1
Giannakakou, P.2
Hamel, E.3
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18
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0027360552
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Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations
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Jordan MA, Toso RJ, Thrower D, Wilson L. Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. Proc Natl Acad Sci USA. 90:1993;9552-9556.
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(1993)
Proc Natl Acad Sci USA
, vol.90
, pp. 9552-9556
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Jordan, M.A.1
Toso, R.J.2
Thrower, D.3
Wilson, L.4
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19
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0029115509
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Vinblastine suppresses dynamics of individual microtubules in living cells
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Dhamodharan RI, Jordan MA, Thrower D, Wilson L, Wadsworth P. Vinblastine suppresses dynamics of individual microtubules in living cells. Mol Biol Cell. 6:1995;1215-1229.
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(1995)
Mol Biol Cell
, vol.6
, pp. 1215-1229
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Dhamodharan, R.I.1
Jordan, M.A.2
Thrower, D.3
Wilson, L.4
Wadsworth, P.5
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20
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0029923972
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Differential effects of vinblastine on polymerization and dynamics at opposite microtubule ends
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Panda D, Jordan MA, Chin K, Wilson L. Differential effects of vinblastine on polymerization and dynamics at opposite microtubule ends. J Biol Chem. 271:1996;29807-29812.
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(1996)
J Biol Chem
, vol.271
, pp. 29807-29812
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Panda, D.1
Jordan, M.A.2
Chin, K.3
Wilson, L.4
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21
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0029155318
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Kinetic stabilization of microtubule dynamics at steady state in vitro by substoichiometric concentrations of tubulin-colchicine complex
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Panda D, Daijo JE, Jordan MA, Wilson L. Kinetic stabilization of microtubule dynamics at steady state in vitro by substoichiometric concentrations of tubulin-colchicine complex. Biochemistry. 34:1995;9921-9929.
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(1995)
Biochemistry
, vol.34
, pp. 9921-9929
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-
Panda, D.1
Daijo, J.E.2
Jordan, M.A.3
Wilson, L.4
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22
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0030721014
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Unusually potent suppression of microtubule dynamics by crytophycin 1: A mechanism for its antiproliferative action
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Panda D, Himes D, Himes RH, Moore RE, Wilson L, Jordan MA. Unusually potent suppression of microtubule dynamics by crytophycin 1: a mechanism for its antiproliferative action. Biochemistry. 36:1997;12948-12953.
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(1997)
Biochemistry
, vol.36
, pp. 12948-12953
-
-
Panda, D.1
Himes, D.2
Himes, R.H.3
Moore, R.E.4
Wilson, L.5
Jordan, M.A.6
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23
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0030920202
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Nanomolar concentrations on nocodazole alter microtubule dynamic instability in vivo and in vitro
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of special interest. Here the authors show that he compound nocodazole, a benzimidazole derivative that arrests mitosis, suppresses microtubule dynamics in living cells in interphase without depolymerizing microtubules.
-
Vasquez RJ, Howell B, Yvon A-MC, Wadsworth P, Cassimeris L. Nanomolar concentrations on nocodazole alter microtubule dynamic instability in vivo and in vitro. of special interest Mol Biol Cell. 8:1997;973-985 Here the authors show that he compound nocodazole, a benzimidazole derivative that arrests mitosis, suppresses microtubule dynamics in living cells in interphase without depolymerizing microtubules.
-
(1997)
Mol Biol Cell
, vol.8
, pp. 973-985
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Vasquez, R.J.1
Howell, B.2
Yvon A-Mc3
Wadsworth, P.4
Cassimeris, L.5
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24
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0030886376
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Stabilization of microtubule dynamics by estramustine by binding to a novel site in tubulin: A possible mechanistic basis for its antitumor action
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of special interest. Shows additive stabilization of microtubule dynamics by low concentrations on vinblastine and estramustine.
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Panda D, Miller H, Islam K, Wilson L. Stabilization of microtubule dynamics by estramustine by binding to a novel site in tubulin: A possible mechanistic basis for its antitumor action. of special interest Proc Natl Acad Sci USA. 94:1997;10560-10564 Shows additive stabilization of microtubule dynamics by low concentrations on vinblastine and estramustine.
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(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 10560-10564
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Panda, D.1
Miller, H.2
Islam, K.3
Wilson, L.4
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25
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0028908695
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Substoichiometric binding of taxol suppresses microtubule dynamics
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Derry WB, Wilson L, Jordan MA. Substoichiometric binding of taxol suppresses microtubule dynamics. Biochemistry. 34:1995;2203-2211.
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(1995)
Biochemistry
, vol.34
, pp. 2203-2211
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Derry, W.B.1
Wilson, L.2
Jordan, M.A.3
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26
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0025824979
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Mechanism of inhibition of cell proliferation by Vinca alkaloids
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Jordan MA, Thrower D, Wilson L. Mechanism of inhibition of cell proliferation by Vinca alkaloids. Cancer Res. 51:1991;2212-2222.
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(1991)
Cancer Res
, vol.51
, pp. 2212-2222
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Jordan, M.A.1
Thrower, D.2
Wilson, L.3
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27
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0028889363
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Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes
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Zhang D, Nicklas R. Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes. J Cell Biol. 131:1995;1125-1131.
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(1995)
J Cell Biol
, vol.131
, pp. 1125-1131
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Zhang, D.1
Nicklas, R.2
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28
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0027462041
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Mitotic block in HeLa cells by vinblastine: Ultrastructural changes in kinetochore-microtubule attachment and in centrosomes
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Wendell KL, Wilson L, Jordan MA. Mitotic block in HeLa cells by vinblastine: ultrastructural changes in kinetochore-microtubule attachment and in centrosomes. J Cell Sci. 104:1993;261-274.
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(1993)
J Cell Sci
, vol.104
, pp. 261-274
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Wendell, K.L.1
Wilson, L.2
Jordan, M.A.3
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29
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0026643589
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Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis
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Jordan MA, Thrower D, Wilson L. Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis. J Cell Sci. 102:1992;401-416.
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(1992)
J Cell Sci
, vol.102
, pp. 401-416
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Jordan, M.A.1
Thrower, D.2
Wilson, L.3
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30
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0029881977
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Abnormal centrosome amplications in the absence of p53
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of special interest. In this paper it is observed that in mouse embryonic fibroblasts lacking the p53 tumor suppressor protein, multiple copies of functionally competent centrosomes were generated during a single cell cycle, implicating p53 in the regulation of centrosome duplication and suggesting a mechanism by which loss of p53 may cause genetic instability.
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Fukasawa K, Choi T, Kuriyama R, Rulong S, Vande Woude GF. Abnormal centrosome amplications in the absence of p53. of special interest Science. 271:1996;1744-1747 In this paper it is observed that in mouse embryonic fibroblasts lacking the p53 tumor suppressor protein, multiple copies of functionally competent centrosomes were generated during a single cell cycle, implicating p53 in the regulation of centrosome duplication and suggesting a mechanism by which loss of p53 may cause genetic instability.
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(1996)
Science
, vol.271
, pp. 1744-1747
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Fukasawa, K.1
Choi, T.2
Kuriyama, R.3
Rulong, S.4
Vande Woude, G.F.5
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31
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0029836707
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The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work
-
of special interest. Here the authors show that taxol inhibits kinetochore-microtubule assembly/disassembly at kinetochores; however, minus-end microtubule disassembly continued.
-
Waters JC, Mitchison TJ, Rieder CL, Salmon ED. The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work. of special interest Mol Biol Cell. 7:1996;1547-1558 Here the authors show that taxol inhibits kinetochore-microtubule assembly/disassembly at kinetochores; however, minus-end microtubule disassembly continued.
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(1996)
Mol Biol Cell
, vol.7
, pp. 1547-1558
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Waters, J.C.1
Mitchison, T.J.2
Rieder, C.L.3
Salmon, E.D.4
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32
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0032520944
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The low potency of taxol at microtubule minus ends: Implications for its therapeutic mechanism
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in press
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Derry WB, Wilson L, Jordan MA. The low potency of taxol at microtubule minus ends: implications for its therapeutic mechanism. Cancer Res. 1998;. in press.
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(1998)
Cancer Res
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Derry, W.B.1
Wilson, L.2
Jordan, M.A.3
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33
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0028872743
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Mitotic forces control a cell-cycle checkpoint
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Li X, Nicklas RB. Mitotic forces control a cell-cycle checkpoint. Nature. 373:1995;630-632.
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(1995)
Nature
, vol.373
, pp. 630-632
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Li, X.1
Nicklas, R.B.2
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34
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0029146196
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Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint
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Nicklas RB, Ward SC, Gorbsky GJ. Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint. J Cell Biol. 130:1995;929-939.
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(1995)
J Cell Biol
, vol.130
, pp. 929-939
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Nicklas, R.B.1
Ward, S.C.2
Gorbsky, G.J.3
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35
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0029160299
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The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetchores
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Rieder CL, Cole RW, Khodjakov A, Sluder G. The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetchores. J Cell Biol. 130:1995;941-948.
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(1995)
J Cell Biol
, vol.130
, pp. 941-948
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Rieder, C.L.1
Cole, R.W.2
Khodjakov, A.3
Sluder, G.4
-
36
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0029845891
-
Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores
-
of outstanding interest. This paper reports the isolation and expression of a cDNA encoding the Xenopus homolog of MAD2 protein (mitotic-arrest deficient 2 protein). Affinity-purified antibodies to XMAD2 recognized a 25-kD protein localized to unattached kinetochores at prometaphase in nocodazole-treated frog or human cells. The protein disappeared from kinetochores at metaphase in untreated cells, suggesting that XMAD2 plays a role in activation of the checkpoint by unattached kinetochores.
-
Chen R, Waters J, Salmon E, Murray A. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores. of outstanding interest Science. 274:1996;242-246 This paper reports the isolation and expression of a cDNA encoding the Xenopus homolog of MAD2 protein (mitotic-arrest deficient 2 protein). Affinity-purified antibodies to XMAD2 recognized a 25-kD protein localized to unattached kinetochores at prometaphase in nocodazole-treated frog or human cells. The protein disappeared from kinetochores at metaphase in untreated cells, suggesting that XMAD2 plays a role in activation of the checkpoint by unattached kinetochores.
-
(1996)
Science
, vol.274
, pp. 242-246
-
-
Chen, R.1
Waters, J.2
Salmon, E.3
Murray, A.4
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37
-
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10244251532
-
Identification of a human mitotic checkpoint gene: HsMAD2
-
of outstanding interest. Here, the human homolog of MAD2 (hsMAD2) was isolated and shown to be a necessary component of nocodazole-induced mitotic arrest in Hela cells, by electroporation of anti-hsMAD2 antibodies. As in [36], hsMAD2 was localized at the kinetochore in prometaphase in nocodazole-treated cells, but disappeared from kinetochores at metaphase in untreated cells. Human breast tumor cells exhibited reduced hsMAD2 expression and failed to arrest in mitosis after nocodazole treatment, suggesting that defects in MAD2 expression may alter the responsiveness of tumors to antimitotic drugs.
-
Li Y, Benezra R. Identification of a human mitotic checkpoint gene: hsMAD2. of outstanding interest Science. 274:1996;249-249 Here, the human homolog of MAD2 (hsMAD2) was isolated and shown to be a necessary component of nocodazole-induced mitotic arrest in Hela cells, by electroporation of anti-hsMAD2 antibodies. As in [36], hsMAD2 was localized at the kinetochore in prometaphase in nocodazole-treated cells, but disappeared from kinetochores at metaphase in untreated cells. Human breast tumor cells exhibited reduced hsMAD2 expression and failed to arrest in mitosis after nocodazole treatment, suggesting that defects in MAD2 expression may alter the responsiveness of tumors to antimitotic drugs.
-
(1996)
Science
, vol.274
, pp. 249-249
-
-
Li, Y.1
Benezra, R.2
-
38
-
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0030464317
-
Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast
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of special interest. In this paper it is shown that in budding yeast the cdc55 gene, which encodes a protein phosphatase 2A regulatory subunit, shows a checkpoint defect that allows sister chromatids to separate and cells to exit mitosis without inducing cyclin B destruction. This suggests that after drug-blockage at the mitotic checkpoint, cells may bypass the cyclin B proteolytic mechanism and inactivate MPF by phosphorylation of Cdc28.
-
Minshull J, Straight A, Rudner AD, Demburg AF, Belmont A, Murray AW. Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast. of special interest Curr Biol. 6:1996;1609-1620 In this paper it is shown that in budding yeast the cdc55 gene, which encodes a protein phosphatase 2A regulatory subunit, shows a checkpoint defect that allows sister chromatids to separate and cells to exit mitosis without inducing cyclin B destruction. This suggests that after drug-blockage at the mitotic checkpoint, cells may bypass the cyclin B proteolytic mechanism and inactivate MPF by phosphorylation of Cdc28.
-
(1996)
Curr Biol
, vol.6
, pp. 1609-1620
-
-
Minshull, J.1
Straight, A.2
Rudner, A.D.3
Demburg, A.F.4
Belmont, A.5
Murray, A.W.6
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39
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0030474371
-
GFP tagging of budding yeast chromosomes reveals that protein - Protein interactions can mediate sister chromatid cohesion
-
of special interest. In mitotic checkpoint-deficient yeast mutants mad and bub, this study shows sister chromatid separation can occur in the absence of microtubules (after microtubule depolymerization by high concentrations of the mitotic inhibitor nocodazole), indicating that sister chromatic separation is under the control of the spindle-assembly checkpoint and can occur in the absence of microtubule-dependent forces.
-
Straight A, Belmont A, Robinett CC, Murray AW. GFP tagging of budding yeast chromosomes reveals that protein - protein interactions can mediate sister chromatid cohesion. of special interest Curr Biol. 6:1996;1599-1608 In mitotic checkpoint-deficient yeast mutants mad and bub, this study shows sister chromatid separation can occur in the absence of microtubules (after microtubule depolymerization by high concentrations of the mitotic inhibitor nocodazole), indicating that sister chromatic separation is under the control of the spindle-assembly checkpoint and can occur in the absence of microtubule-dependent forces.
-
(1996)
Curr Biol
, vol.6
, pp. 1599-1608
-
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Straight, A.1
Belmont, A.2
Robinett, C.C.3
Murray, A.W.4
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40
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0030561511
-
The spindle assembly checkpoint
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Rudner AD, Murray AW. The spindle assembly checkpoint. Curr Biol. 8:1996;773-780.
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(1996)
Curr Biol
, vol.8
, pp. 773-780
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Rudner, A.D.1
Murray, A.W.2
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41
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0030981660
-
Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset
-
of outstanding interest. By same-cell video light microscopy/serial section electron microscopy of PtK1 cells the authors of this report found that treatment of late metaphase cells with 1 μM taxol inhibits anaphase onset, but does not alter the number of kinetochore microtubules, suggesting that inhibition of anaphase onset by taxol involves suppression of microtubule dynamics rather than inhibition of microtubule attachment to kinetochores.
-
McEwen B, Heagle A, Cassels G, Buttle K, Rieder C. Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset. of outstanding interest J Cell Biol. 137:1997;1567-1580 By same-cell video light microscopy/serial section electron microscopy of PtK1 cells the authors of this report found that treatment of late metaphase cells with 1 μM taxol inhibits anaphase onset, but does not alter the number of kinetochore microtubules, suggesting that inhibition of anaphase onset by taxol involves suppression of microtubule dynamics rather than inhibition of microtubule attachment to kinetochores.
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(1997)
J Cell Biol
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McEwen, B.1
Heagle, A.2
Cassels, G.3
Buttle, K.4
Rieder, C.5
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0026802011
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Colombel M, Olsson CA, Ng PY, Buttyan R. Hormone-regulated apoptosis results from reentry of differentiated prostate cells onto a defective cell cycle. Cancer Res. 52:1992;4313-4319.
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Colombel, M.1
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Ng, P.Y.3
Buttyan, R.4
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43
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0030020158
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Mitotic block induced in HeLa cells by low concentration of paclitaxel (Taxol) results in abnormal mitotic exit and apoptotic cell death
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of special interest. In this paper the authors triggered apoptosis in HeLa cells, following mitotic block, by low nanomolar concentations of taxol that appear to suppress microtubule dynamics but do not bundle microtubules. Following removal of taxol, cells entered an interphase-like state without progressing through anaphase or cytokinesis and underwent apoptosis.
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Jordan MA, Wendell KL, Gardiner S, Derry WB, Copp H, Wilson L. Mitotic block induced in HeLa cells by low concentration of paclitaxel (Taxol) results in abnormal mitotic exit and apoptotic cell death. of special interest Cancer Res. 56:1996;816-825 In this paper the authors triggered apoptosis in HeLa cells, following mitotic block, by low nanomolar concentations of taxol that appear to suppress microtubule dynamics but do not bundle microtubules. Following removal of taxol, cells entered an interphase-like state without progressing through anaphase or cytokinesis and underwent apoptosis.
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Cancer Res
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Jordan, M.A.1
Wendell, K.L.2
Gardiner, S.3
Derry, W.B.4
Copp, H.5
Wilson, L.6
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44
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0027196777
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Taxol induces internucleosomal DNA fragmentation associated with programmed cell death in human myeloid leukemia cells
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Bhalla K, Ilerado AM, Tourkina E, Tang C, Mahoney ME, Huang Y. Taxol induces internucleosomal DNA fragmentation associated with programmed cell death in human myeloid leukemia cells. Leukemia. 7:1993;563-568.
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Bhalla, K.1
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Tourkina, E.3
Tang, C.4
Mahoney, M.E.5
Huang, Y.6
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46
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0028983396
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Paclitaxel (taxol) inhibits protein isoprenylation and induces apoptosis in PC-3 human prostate cancer cells
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Danesi R, Figg WD, Reed E, Myers CE. Paclitaxel (taxol) inhibits protein isoprenylation and induces apoptosis in PC-3 human prostate cancer cells. Mol Pharmacol. 47:1995;1106-1111.
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Danesi, R.1
Figg, W.D.2
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47
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Disruption of microtubules induces an endogenous suicide pathway in human leukaemia HL-60 cells
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Martin SJ, Cotter TG. Disruption of microtubules induces an endogenous suicide pathway in human leukaemia HL-60 cells. Cell Tissue Kinet. 23:1990;545-559.
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Martin, S.J.1
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48
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0029328641
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Taxol-induced mitotic block triggers rapid onset of a p53-independent apoptotic pathway
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Woods CM, Zhu J, McQueney PA, Bollag D, Lazarides E. Taxol-induced mitotic block triggers rapid onset of a p53-independent apoptotic pathway. Mol Med. 1:1995;1076-1551.
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Mol Med
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Woods, C.M.1
Zhu, J.2
McQueney, P.A.3
Bollag, D.4
Lazarides, E.5
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49
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0029913172
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Taxol-induced apoptosis and phosphorylation of bcl-2 protein involves c-Raf-1 and represents a novel c-Raf-1 signal transduction pathway
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of special interest. In this paper the authors demonstrate taxol-induced phosphorylation and activation of the Raf-1 mitogen-stimulated protein kinase, coincident with phosphorylation of bcl-2. Depletion of Raf-1 protein by benzoquinone ansamycin GA prevented taxol-induced apoptosis and bcl-2 phosphorylation.
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Blagosklonny MV, Schulte T, Nguyen P, Trepel J, Neckers L. Taxol-induced apoptosis and phosphorylation of bcl-2 protein involves c-Raf-1 and represents a novel c-Raf-1 signal transduction pathway. of special interest Cancer Res. 56:1996;1851-1854 In this paper the authors demonstrate taxol-induced phosphorylation and activation of the Raf-1 mitogen-stimulated protein kinase, coincident with phosphorylation of bcl-2. Depletion of Raf-1 protein by benzoquinone ansamycin GA prevented taxol-induced apoptosis and bcl-2 phosphorylation.
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(1996)
Cancer Res
, vol.56
, pp. 1851-1854
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Blagosklonny, M.V.1
Schulte, T.2
Nguyen, P.3
Trepel, J.4
Neckers, L.5
-
50
-
-
0031036307
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Raf-1/bcl2 phosphorylation: A step from microtubule damage to cell death
-
of special interest. Here, it is reported that to taxol, vinca alkaloids, colchicine, epothilones and other microtubule active agents induce Raf-1/bcl-2 phosphorylation.
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Blagosklonny MV, Giannakakou P, El-Deiry WS, Kingston DGI, Higgs PL, Neckers L, Fojo T. Raf-1/bcl2 phosphorylation: a step from microtubule damage to cell death. of special interest Cancer Res. 57:1997;130-135 Here, it is reported that to taxol, vinca alkaloids, colchicine, epothilones and other microtubule active agents induce Raf-1/bcl-2 phosphorylation.
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(1997)
Cancer Res
, vol.57
, pp. 130-135
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Blagosklonny, M.V.1
Giannakakou, P.2
El-Deiry, W.S.3
Kingston, D.G.I.4
Higgs, P.L.5
Neckers, L.6
Fojo, T.7
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51
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0031035693
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Bcl2 is the guardian of microtubule integrity
-
of special interest. The authors of this report observed that drugs that affect microtubules, including taxotere (1-100 nM), vinblastine, and vincristine (100 nM), induce phosphorylation of bcl2 in G2/M and induce apoptosis in human leukemia, lymphoma, breast and prostate cancer cells.
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Haldar S, Basu A, Croce CM. Bcl2 is the guardian of microtubule integrity. of special interest Cancer Res. 57:1997;229-233 The authors of this report observed that drugs that affect microtubules, including taxotere (1-100 nM), vinblastine, and vincristine (100 nM), induce phosphorylation of bcl2 in G2/M and induce apoptosis in human leukemia, lymphoma, breast and prostate cancer cells.
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(1997)
Cancer Res
, vol.57
, pp. 229-233
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Haldar, S.1
Basu, A.2
Croce, C.M.3
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52
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0029807822
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Differential taxol-dependent arrest of transformed and nontransformed cells in the G1 phase of the cell cycle, and specific-related mortality of transformed cells
-
of special interest. In this paper evidence is presented showing that taxol, but not nocodazole, at micromolar concentrations, may induce a G1 block from which nontransformed rat and human fibroblasts recover and proliferate after release from taxol; however, transformed cells of the same lineage slip through the checkpoint and undergo apoptotis. The results suggest the existence of a microtubule-dependent checkpoint in G1 which is abrogated in transformed cells.
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Trielli M, Andreasson PR, Lacroix FB, Margolis RL. Differential taxol-dependent arrest of transformed and nontransformed cells in the G1 phase of the cell cycle, and specific-related mortality of transformed cells. of special interest J Cell Biol. 135:1996;689-700 In this paper evidence is presented showing that taxol, but not nocodazole, at micromolar concentrations, may induce a G1 block from which nontransformed rat and human fibroblasts recover and proliferate after release from taxol; however, transformed cells of the same lineage slip through the checkpoint and undergo apoptotis. The results suggest the existence of a microtubule-dependent checkpoint in G1 which is abrogated in transformed cells.
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(1996)
J Cell Biol
, vol.135
, pp. 689-700
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Trielli, M.1
Andreasson, P.R.2
Lacroix, F.B.3
Margolis, R.L.4
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53
-
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0030929838
-
DNA rereplication in the presence of mitotic spindle inhibitors in human and mouse fibroblasts lacking either p53 or pRb function
-
of special interest. This group found that normal mouse and human fibroblasts arrested with a 4N DNA content when treated with nocodazole and Colcemid, whereas isogeneic p53-deficient or pRb-deficient derivatives became polyploid indicating different susceptibilities of normal and mutated cells to anti-mitotic drugs.
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Di Leonardo A, Khan S, Linke S, Greco V, Seidita G, Wahl G. DNA rereplication in the presence of mitotic spindle inhibitors in human and mouse fibroblasts lacking either p53 or pRb function. of special interest Cancer Res. 57:1997;1013-1019 This group found that normal mouse and human fibroblasts arrested with a 4N DNA content when treated with nocodazole and Colcemid, whereas isogeneic p53-deficient or pRb-deficient derivatives became polyploid indicating different susceptibilities of normal and mutated cells to anti-mitotic drugs.
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(1997)
Cancer Res
, vol.57
, pp. 1013-1019
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Di Leonardo, A.1
Khan, S.2
Linke, S.3
Greco, V.4
Seidita, G.5
Wahl, G.6
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54
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0030846513
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Cellular targets of the anti-breast cancer agent Z-1, 1-dichloro-2,3-diphenylcyclopropane; Type II estrogen binding sites and tubulin
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Ter Harr E, Hamel E, Balachandran R, Day B. Cellular targets of the anti-breast cancer agent Z-1, 1-dichloro-2,3-diphenylcyclopropane; type II estrogen binding sites and tubulin. Anticancer Res. 17:1997;1861-1869.
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Ter Harr, E.1
Hamel, E.2
Balachandran, R.3
Day, B.4
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55
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Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance
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Zhang X, Smith CD. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol Pharmacol. 49:1996;288-294.
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Smith, C.D.2
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56
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Suppression of microtubule dynamics by LY290181
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Panda S, Singh JP, Wilson L. Suppression of microtubule dynamics by LY290181. J Biol Chem. 272:1997;7681-7687.
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Panda, S.1
Singh, J.P.2
Wilson, L.3
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57
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Inhibition of mitosis and microtubule function through direct tubulin binding by a novel antiproliferative naphthopyran LY290181
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Wood DL, Panda D, Wiernicki TR, Wilson L, Jordan MA, Singh JP. Inhibition of mitosis and microtubule function through direct tubulin binding by a novel antiproliferative naphthopyran LY290181. Mol Pharmacol. 52:1997;437-444.
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Wood, D.L.1
Panda, D.2
Wiernicki, T.R.3
Wilson, L.4
Jordan, M.A.5
Singh, J.P.6
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58
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0010608767
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Antimitotic and tubulin interacting properties of a novel fluorinated vinca alkaloid, vinflunine
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in press
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Kruczynski A, Barret J-M, Etievant C, Colpaert F, Fahy J, Hill BT. Antimitotic and tubulin interacting properties of a novel fluorinated vinca alkaloid, vinflunine. Biochem Pharmacol. 1997;. in press.
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Biochem Pharmacol
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Kruczynski, A.1
Barret J-M2
Etievant, C.3
Colpaert, F.4
Fahy, J.5
Hill, B.T.6
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59
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0029759374
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Characterization of the interaction of crytophycin 1 with tubulin: Binding in the Vinca domain, competitive inhibition to dolastatin 10 binding, and an unusual aggregation reaction
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Bai R, Schwartz RE, Kepler JA, Pettit GR, Hamel E. Characterization of the interaction of crytophycin 1 with tubulin: binding in the Vinca domain, competitive inhibition to dolastatin 10 binding, and an unusual aggregation reaction. Cancer Res. 56:1996;4398-4406.
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Bai, R.1
Schwartz, R.E.2
Kepler, J.A.3
Pettit, G.R.4
Hamel, E.5
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60
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0029874514
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Mechanism of action of cryptophycin. Interaction with the vinca alkaloid domain of tubulin
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Smith CD, Zhang X. Mechanism of action of cryptophycin. Interaction with the vinca alkaloid domain of tubulin. J Biol Chem. 271:1996;6192-6196.
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Smith, C.D.1
Zhang, X.2
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61
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Kerksiek K, Mejillano MR, Schwartz RE, Georg GI, Himes RH. Interaction of cryptophycin 1 with tubulin and microtubules. FEBS Letts. 377:1995;59-61.
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Mejillano, M.R.2
Schwartz, R.E.3
Georg, G.I.4
Himes, R.H.5
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62
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0031106360
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In vitro synergy of paclitaxel (Taxol) and vinorelbine (navelbine) against human melanoma cell lines
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of special interest. Here the authors report that adding taxol and vinorelbine, a synthetic vinca alkaloid, at low nanomolar concentrations concurrently to cultures of human melanoma cell lines, resulted in supra-additive or synergistic antiproliferative effects.
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Photiou A, Shah P, Leong L, Moss J, Retsas S. In vitro synergy of paclitaxel (Taxol) and vinorelbine (navelbine) against human melanoma cell lines. of special interest Eur J Cancer. 33:1997;463-470 Here the authors report that adding taxol and vinorelbine, a synthetic vinca alkaloid, at low nanomolar concentrations concurrently to cultures of human melanoma cell lines, resulted in supra-additive or synergistic antiproliferative effects.
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Eur J Cancer
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Photiou, A.1
Shah, P.2
Leong, L.3
Moss, J.4
Retsas, S.5
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63
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0029103212
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Simultaneous combination of microtubule depolymerizing and stabilizing agents acts at low doses
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Garcia P, Braguer D, Carles G, Briand C. Simultaneous combination of microtubule depolymerizing and stabilizing agents acts at low doses. Anti-Cancer Drug. 6:1995;533-544.
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Anti-Cancer Drug
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Garcia, P.1
Braguer, D.2
Carles, G.3
Briand, C.4
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64
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0026808448
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Combined antimicrotubule activity of estramustine and taxol in human prostatic carcinoma cell lines
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Speicher LA, Barone L, Tew KD. Combined antimicrotubule activity of estramustine and taxol in human prostatic carcinoma cell lines. Cancer Res. 52:1992;4433-4440.
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Cancer Res
, vol.52
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Speicher, L.A.1
Barone, L.2
Tew, K.D.3
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65
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0030940117
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Taxol differentially modulates the dynamics and microtubules assembled from unfractionated and purified beta-tubulin isotypes
-
of special interest. In this report the authors report that microtubules composed of purified αβ III or αβIV bovine brain tubulin were sevenfold less sensitive to the suppressive effects of bound taxol on the rate of shortening during excursions of dynamic instability, suggesting that cellular expression of increased levels of βIII or βIV tubulin isotypes may be an important mechanism of tumor cell resistance to taxol.
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Derry WB, Wilson L, Khan IA, Luduena RF, Jordan MA. Taxol differentially modulates the dynamics and microtubules assembled from unfractionated and purified beta-tubulin isotypes. of special interest Biochemistry. 36:1997;3554-3562 In this report the authors report that microtubules composed of purified αβ III or αβIV bovine brain tubulin were sevenfold less sensitive to the suppressive effects of bound taxol on the rate of shortening during excursions of dynamic instability, suggesting that cellular expression of increased levels of βIII or βIV tubulin isotypes may be an important mechanism of tumor cell resistance to taxol.
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Biochemistry
, vol.36
, pp. 3554-3562
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Derry, W.B.1
Wilson, L.2
Khan, I.A.3
Luduena, R.F.4
Jordan, M.A.5
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66
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0031039994
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Interaction of estramustine with tubulin isotypes
-
of special interest. This paper documents the finding that binding of estramustine to the βIII isotype of tubulin occurs with reduced efficacy as compared with other β-tubulin isotypes, thus suggesting a mechanism for the resistance to estramustine of cancer cells overexpressing the βIII isotype of tubulin (see reference [69]).
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Laing N, Dahllof B, Hartley-Asp B, Ranganathan S, Tew KD. Interaction of estramustine with tubulin isotypes. of special interest Biochemistry. 36:1997;871-878 This paper documents the finding that binding of estramustine to the βIII isotype of tubulin occurs with reduced efficacy as compared with other β-tubulin isotypes, thus suggesting a mechanism for the resistance to estramustine of cancer cells overexpressing the βIII isotype of tubulin (see reference [69]).
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Biochemistry
, vol.36
, pp. 871-878
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Laing, N.1
Dahllof, B.2
Hartley-Asp, B.3
Ranganathan, S.4
Tew, K.D.5
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67
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13144256456
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Taxol-resistant epithelial ovarian tumors are associated with altered expression of specific beta-tubulin isotypes
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In this report the authors observed that taxol-resistant human ovarian tumors displayed significant increases in expression of class I, III and IVa β-tubulins, when compared to untreated primary ovarian tumors. Taxol-resistant cultured human lung cancer cells are also showed increases in the levels of classes, I,II,III, and IVa β tubulins, although MDR expression, total tubulin levels, and drug.
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Kavalleris M, Kuo DY-S, Burkhardt CA, Regl DL, Norris MD, Haber M, Horwitz SB. Taxol-resistant epithelial ovarian tumors are associated with altered expression of specific beta-tubulin isotypes. J Clin Invest. 100:1997;1-12 In this report the authors observed that taxol-resistant human ovarian tumors displayed significant increases in expression of class I, III and IVa β-tubulins, when compared to untreated primary ovarian tumors. Taxol-resistant cultured human lung cancer cells are also showed increases in the levels of classes, I,II,III, and IVa β tubulins, although MDR expression, total tubulin levels, and drug.
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J Clin Invest
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Kavalleris, M.1
Kuo Dy-S2
Burkhardt, C.A.3
Regl, D.L.4
Norris, M.D.5
Haber, M.6
Horwitz, S.B.7
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68
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0029550441
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Novel mechanism of resistance of paclitaxel in human K562 leukemia cells by combined selection with PSC833
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Jaffrezou J-P, Dumontet C, Derry WB, Duran G, Tsuchiya E, Wilson L, Jordan MA, Sikic B. Novel mechanism of resistance of paclitaxel in human K562 leukemia cells by combined selection with PSC833. Oncology Res. 7:1995;517-527.
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Jaffrezou J-P1
Dumontet, C.2
Derry, W.B.3
Duran, G.4
Tsuchiya, E.5
Wilson, L.6
Jordan, M.A.7
Sikic, B.8
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69
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0029973041
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Increase of βiII- And βiVa-tubulin isotypes in human prostate carcinoma cells as a result of estramustine resistance
-
of special interest. The authors of this paper report that human prostate carcinoma cells (DU145), selected for resistance to estramustine, were moderately cross-resistant to paclitaxel, and overexpressed two β-tubulin isotypes three- to fourfold (classes IVa and III), suggesting that altered expression of tubulin isotypes may play a role in cellular resistance to antimicrotubule drugs.
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Ranganathan S, Dexter DW, Benetatos CA, Chapman AE, Tew K, Hudes GR. Increase of βIII- and βIVa-tubulin isotypes in human prostate carcinoma cells as a result of estramustine resistance. of special interest Cancer Res. 56:1996;2584-2589 The authors of this paper report that human prostate carcinoma cells (DU145), selected for resistance to estramustine, were moderately cross-resistant to paclitaxel, and overexpressed two β-tubulin isotypes three- to fourfold (classes IVa and III), suggesting that altered expression of tubulin isotypes may play a role in cellular resistance to antimicrotubule drugs.
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Cancer Res
, vol.56
, pp. 2584-2589
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Ranganathan, S.1
Dexter, D.W.2
Benetatos, C.A.3
Chapman, A.E.4
Tew, K.5
Hudes, G.R.6
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70
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Resistance mechanisms in human sarcoma mutants derived by single-step exposure to paclitaxel (Taxol)
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of special interest. In this report single-step exposure of MES-SA sarcoma cells to paclitaxel induced formation of 5 Mdr1-negative clones that were resistant to taxol, cross-resistant to vinca alkaloids, and underexpressed 2 β-tubulin isotypes, β5 (class IVa) and β4 (class III), as determined by RT-PCR, which suggests that altered microtubule function is responsible for resistance to taxol in these cells.
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Dumontet C, Duran G, Steger K, Beketic-Oreskovic L, Sikic B. Resistance mechanisms in human sarcoma mutants derived by single-step exposure to paclitaxel (Taxol). of special interest Cancer Res. 56:1996;1091-1097 In this report single-step exposure of MES-SA sarcoma cells to paclitaxel induced formation of 5 Mdr1-negative clones that were resistant to taxol, cross-resistant to vinca alkaloids, and underexpressed 2 β-tubulin isotypes, β5 (class IVa) and β4 (class III), as determined by RT-PCR, which suggests that altered microtubule function is responsible for resistance to taxol in these cells.
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(1996)
Cancer Res
, vol.56
, pp. 1091-1097
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Dumontet, C.1
Duran, G.2
Steger, K.3
Beketic-Oreskovic, L.4
Sikic, B.5
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71
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0030758777
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Paclitaxel-resistant human ovarian cancer cells have mutant beta-tubulins that exhibit impaired paclitaxel-driven polymerization
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of outstanding interest. Here, the authors report that purified tubulin from two taxol-resistant sublines of human ovarian carcinoma cells exhibited resistance to taxol-induced polymerization which was attributed to acquired point mutations in the M40 isotype. The cell lines were sensitive to vinblastine, accumulated paclitaxel equally with the parental cells, expressed no detectable MDR-1 RNA, and expressed β-tubulin isotypes similarly, with a 1-5-fold increase in expression of M40 (betal) RNA.
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Giannakakou P, Sackett D, Kang Y, Zhan Z, Buters J, Fojo T, Poruchynsky M. Paclitaxel-resistant human ovarian cancer cells have mutant beta-tubulins that exhibit impaired paclitaxel-driven polymerization. of outstanding interest J Biol Chem. 272:1997;17118-17125 Here, the authors report that purified tubulin from two taxol-resistant sublines of human ovarian carcinoma cells exhibited resistance to taxol-induced polymerization which was attributed to acquired point mutations in the M40 isotype. The cell lines were sensitive to vinblastine, accumulated paclitaxel equally with the parental cells, expressed no detectable MDR-1 RNA, and expressed β-tubulin isotypes similarly, with a 1-5-fold increase in expression of M40 (betal) RNA.
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J Biol Chem
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Giannakakou, P.1
Sackett, D.2
Kang, Y.3
Zhan, Z.4
Buters, J.5
Fojo, T.6
Poruchynsky, M.7
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72
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Mechanisms by which mammalian cells acquire resistance to drugs that affect microtubule assembly
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Cabral F, Barlow SB. Mechanisms by which mammalian cells acquire resistance to drugs that affect microtubule assembly. FASEB J. 3:1989;1593-1599.
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Cabral, F.1
Barlow, S.B.2
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73
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0030968633
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The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation
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of special interest. In this experiment, microinjection of recombinant Op18 induced disassembly of microtubules in cells and in vitro. Aspartate substitution of a single serine residue (Ser63) abolished the effect on cellular microtubules.
-
Horwitz S, Shen H, He L, Dittmar P, Neef R, Chen J, Schubart U. The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation. of special interest J Biol Chem. 272:1997;8129-8132 In this experiment, microinjection of recombinant Op18 induced disassembly of microtubules in cells and in vitro. Aspartate substitution of a single serine residue (Ser63) abolished the effect on cellular microtubules.
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J Biol Chem
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Horwitz, S.1
Shen, H.2
He, L.3
Dittmar, P.4
Neef, R.5
Chen, J.6
Schubart, U.7
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74
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0030048731
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Identification of a protein that interacts with tubulin and increases the catastrophe rate of microtubules
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of special interest. This report documents the purification of OP18 from calf thymus extracts and the finding that it is a 17 kDa protein that it appears to bind tubulin dimers; destabilize microtubules, increase their catastrophe rates and decrease their growth rates. OP18 inhibited the taxol (300 μM)-induced polymerization of microtubules. OP18 is therefore a plausible regulator of microtubule dynamics during the cell cycle.
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Belmont LD, Mitchison TJ. Identification of a protein that interacts with tubulin and increases the catastrophe rate of microtubules. of special interest Cell. 84:1996;623-631 This report documents the purification of OP18 from calf thymus extracts and the finding that it is a 17 kDa protein that it appears to bind tubulin dimers; destabilize microtubules, increase their catastrophe rates and decrease their growth rates. OP18 inhibited the taxol (300 μM)-induced polymerization of microtubules. OP18 is therefore a plausible regulator of microtubule dynamics during the cell cycle.
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Cell
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Belmont, L.D.1
Mitchison, T.J.2
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75
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0029811662
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Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics
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of special interest. In this paper it is reported that increased expression of Op 18 resulted in microtubule depolymerization in interphase cells, but mitotic spindles were normal. Expression of Op18 mutated at serines 25 and 38 resulted in an absence of mitotic microtubules.
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Marklund U, Larsson N, Graden H, Brattsand G, Gullberg M. Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics. of special interest EMBO J. 15:1996;5290-5298 In this paper it is reported that increased expression of Op 18 resulted in microtubule depolymerization in interphase cells, but mitotic spindles were normal. Expression of Op18 mutated at serines 25 and 38 resulted in an absence of mitotic microtubules.
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(1996)
EMBO J
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Marklund, U.1
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76
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Regulation of microtubule dynamics of Ca2+/calmodulin-dependent kinase IV/Gr-dependent phosphorylation of oncoprotein 18
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2+/calmodulin-dependent kinase IV/Gr can suppress the microtubule regulating activity of Op18. Increased phosphorylation of Op 18 on serine residue 16 is associated with partial degradation of Op18 and an increase in cellular microtubule mass.
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2+/calmodulin-dependent kinase IV/Gr can suppress the microtubule regulating activity of Op18. Increased phosphorylation of Op 18 on serine residue 16 is associated with partial degradation of Op18 and an increase in cellular microtubule mass.
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(1997)
Mol Cell Biol
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Melander Gradin, G.1
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Janmey PA, Chaponnier C. Medical aspects of the actin cytoskeleton. Curr Opin Cell Biol. 7:1995;111-117.
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Sigmond SH. Signal transduction and actin filament organization. Curr Opin Cell Biol. 8:1996;66-73.
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79
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Tapon N, Hall A. Rho, Rac, and Cdc42 GTPases regulate the organizational of the actin cytoskeleton. Curr Opin Cell Biol. 9:1997;86-92.
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Curr Opin Cell Biol
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Assoian RK, Zhu X. Cell anchorage and the cytoskeleton as partners in growth factors dependent cell cycle progression. Curr Opin Cell Biol. 9:1997;93-98.
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81
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Altered actin polymerization dynamics in various malignant cell types: Evidence for different sensitivity to cytochalasin B
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of outstanding interest. In this report the soluble: total actin ratio of maligant cells was found to be 1.37 to 1.81-fold higher than that of normal cells. The viability of maligant cells was also shown to be more strongly affected by cytochalasin B.
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Stournaras C, Stiakaki E, Koukouritaki SB, Theodoropoulos PA, Kalmanti M, Fostinis Y, Gravanis A. Altered actin polymerization dynamics in various malignant cell types: evidence for different sensitivity to cytochalasin B. of outstanding interest Biochem Pharmacol. 52:1996;1339-1346 In this report the soluble: total actin ratio of maligant cells was found to be 1.37 to 1.81-fold higher than that of normal cells. The viability of maligant cells was also shown to be more strongly affected by cytochalasin B.
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Stournaras, C.1
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82
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G-actin as a risk factor and modulatable endpoint for cancer prevention trials
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of outstanding interest. This review article describes evidence indicating that increased levels of unpolymerized actin and decreased levels of polymerized actin accompany development of cancer and that the soluble and polymeric actin levels in cells may be an important biomarker for early detection of cancer.
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Hermstreet GP III, Rao JY, Hurst RE, Bonner RB, Waliszewski P, Grossman HB, Liebert M, Bane B. G-actin as a risk factor and modulatable endpoint for cancer prevention trials. of outstanding interest J Cell Biochem. 25S:1996;197-204 This review article describes evidence indicating that increased levels of unpolymerized actin and decreased levels of polymerized actin accompany development of cancer and that the soluble and polymeric actin levels in cells may be an important biomarker for early detection of cancer.
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J Cell Biochem
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Hermstreet G.P. III1
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Liebert, M.7
Bane, B.8
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83
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Widespread loss of gelsolin in breast cancers of humans, mice, and rats
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of outstanding interest. This paper expands on previous work showing decreased levels of gelsolin in tumor cells. Gelsolin was shown to be absent or greatly reduced in 70% of human breast cancers and in 100% of rat and mouse primary mammary tumors examined. The authors draw the potentially important conclusion that - across three species - partial or complete loss of gelsolin expression is one of the most frequently occurring defects in breast cancer, regardless of etiology. Insufficient gelsolin may impair regulation of the actin filament cytoskeleton and impair various signal transduction pathways involving the actin cytoskeleton, thereby leading to growth and behavioral defects in breast cancer cells by changing their response to external signals.
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Asch HL, Head K, Dong Y, Natoli F, Winston JS, Connolly JL, Asch BB. Widespread loss of gelsolin in breast cancers of humans, mice, and rats. of outstanding interest Cancer Res. 56:1996;4841-4845 This paper expands on previous work showing decreased levels of gelsolin in tumor cells. Gelsolin was shown to be absent or greatly reduced in 70% of human breast cancers and in 100% of rat and mouse primary mammary tumors examined. The authors draw the potentially important conclusion that - across three species - partial or complete loss of gelsolin expression is one of the most frequently occurring defects in breast cancer, regardless of etiology. Insufficient gelsolin may impair regulation of the actin filament cytoskeleton and impair various signal transduction pathways involving the actin cytoskeleton, thereby leading to growth and behavioral defects in breast cancer cells by changing their response to external signals.
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Asch, H.L.1
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Cooper JA. Effects of cytochalasin and phalloidin on actin. J Cell Biol. 105:1987;1473-1477.
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Spector I, Shochet NR, Blasberger D, Kashman Y. Lantrunculins - novel marine macrolides that disrupt microfilament organization and accct cell growth: 1. Comparison with cytochalasin D. Cell Motil Cytoskeleton. 13:1989;127-144.
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87
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High rates of actin filament turnover in budding yeast and roles for actin is establishment and maintenance of cell polarity revealed using the actin inhibitor lantrunculin-A
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of outstanding interest. This paper reports that the actin assembly-inhibitor lantrunculin A caused complete disruption of actin cytoskeleton in budding yeast within 2-5 min of addition, indicating that the actin cytoskeleton in this non-motile organism is highly dynamic. The study revealed the importance of actin dynamics in a number of yeast cell processes, including localization of secretory vesicles during localized exocytosis
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Ayscough KR, Stryker J, Pokala N, Sanders M, Crews P, Drubin DG. High rates of actin filament turnover in budding yeast and roles for actin is establishment and maintenance of cell polarity revealed using the actin inhibitor lantrunculin-A. of outstanding interest J Cell Biol. 137:1997;399-416 This paper reports that the actin assembly-inhibitor lantrunculin A caused complete disruption of actin cytoskeleton in budding yeast within 2-5 min of addition, indicating that the actin cytoskeleton in this non-motile organism is highly dynamic. The study revealed the importance of actin dynamics in a number of yeast cell processes, including localization of secretory vesicles during localized exocytosis.
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88
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Bubb MR, Senderowicz AMJ, Duncan KIK, Korn ED. Jasplakinolide, a cytotoxic natural product induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin. J Biol Chem. 269:1994;14869-14871.
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Senderowicz AM, Kaur G, Sainz E, Laing C, Inman WD, Rodriguez J, Crews P, Malspeis L, Grever MR, Sausville EA, et al. Jasplakinolide's inhibition of the growth of prostate carcinoma cells in vitro with disruption of the actin cytoskeleton. J Natl Cancer Inst. 87:1995;46-51.
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Senderowicz, A.M.1
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Bubb MR, Spector I, Bershadsky AD, Kom ED. Swimholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. J Biol Chem. 270:1995;3463-3466.
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Terry DR, Spector I, Higa T, Bubb MR. Misakinolide A is a marine macrolide that caps but does not sever filamentous actin. J Biol Chem. 272:1997;7841-7845.
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O'Brien ET, Asai DJ, Jacobs RS, Wilson L. Selective inhibition of cytokinesis in sea urchin embryos by low concentrations of stypoldione, a marine natural product that reacts with sulfhydryl groups. Ann Biochem. 181:1989;51-58.
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L, is independent of p53 status, and is inhibited by a myristylated form of RhoB.
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