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Cytoskeletal integrity is required throughout the mitogen stimulation phase of the cell cycle and mediates the anchorage-dependent expression of cyclin D1
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1 phase overlaps with the growth factor dependent portion. Northern and Western blotting were then used to demonstrate that the spread cell shape, rather than adhesion per se, is required for growth factor dependent induction of cyclin D1 gene expression and phosphorylation of pRb.
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