Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro

Molecular Biology of the Cell

Volumn 8, Issue 6, 1997, Pages 973-985

  Vasquez, Robert J ^a,c   Howell, Bonnie ^a   Yvon, Anne Marie C ^b   Wadsworth, Patricia ^b   Cassimeris, Lynne ^a  

^a LEHIGH UNIVERSITY   (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE; NOCODAZOLE; TUBULIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; LUNG ALVEOLUS EPITHELIUM; MICROTUBULE; MICROTUBULE ASSEMBLY; NEWT; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY;

ANIMALIA; SALAMANDRIDAE;

EID: 0030920202     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.6.973     Document Type: Article

References (53)

1. Bayley, P.M., Schlistra, M.J., and Martin, S.R. (1990). Microtubule dynamic instability: numerical simulation of microtubule transition properties using a lateral cap model. J. Cell Sci. 95, 33-48.
2. Bayley, P.M., Sharma, K.K., and Martin, S.R. (1994). Microtubule dynamics in vitro. In: Microtubules, ed. J.S. Hyams and C.W. Lloyd, New York: Wiley-Liss, 111-137.
3. Belmont, L., and Mitchison, T.J. (1996). Identification of a protein that interacts with tubulin dimers and increases the catastrophe rates of microtubules. Cell 84, 623-631.
4. Caplow, M. (1992). Microtubule dynamics. Curr. Opin. Cell Biol. 4, 58-65.
5. Caplow, M., and Shanks, J. (1995). Induction of microtubule catastrophe by formation of tubulin-GDP and apotubulin subunits at microtubule ends. Biochemistry 34, 15732-15741.
6. Caplow, M., and Shanks, J. (1996). Evidence that a single monolayer of tubulin-GTP cap is both necessary and sufficient to stabilize microtubules. Mol. Biol. Cell 7, 663-675.
7. Carlier, M.F., Didry, D., and Pantaloni, D. (1987). Microtubule elongation and guanosine 5′-triphosphate hydrolysis: role of guanine nucleotides in microtubule dynamics. Biochemistry 26, 4428-4437.
8. Cassimeris, L. (1993). Regulation of dynamic instability. Cell Motil. Cytoskeleton 26, 275-281.
9. Cassimeris, L., Pryer, N.K., and Salmon, E.D. (1988). Real-time observations of microtubule dynamic instability in living cells. J. Cell Biol. 107, 2223-2231.
10. Cole, N., and Lippincott-Schwartz, J. (1995). Organization of organelles and membrane traffic by microtubules. Curr. Opin. Cell Biol. 7, 55-64.
11. DeBrabander, M.J., Van De Veire, R.M.L., Aerts, F.E.M., Borgers, M., and Jannsen, P.A. (1976). The effects of methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl] carbamate (R 17,943: NSC 238159), a new synthetic antitumoral drug interfering with microtubules, on mammalian cells cultured in vitro. Cancer Res. 36, 905-916.
12. Drechsel, D.N., and Kirschner, M.W. (1994). The minimum GTP cap required to stabilize microtubules. Curr. Biol. 4, 1053-1061.
13. Dhamodharan, R., Jordan, M.A., Thrower, D., Wilson, L., and Wadsworth, P. (1995). Vinblastine suppresses dynamics of individual microtubules in living interphase cells. Mol. Biol. Cell 6, 1215-1229.
14. Dhamodharan, R., and Wadsworth, P. (1995). Modulation of microtubule dynamic instability in vivo by brain microtubule associated proteins. J. Cell Sci. 108, 1679-1689.
15. Erickson, H.P., and O'Brien, E.T. (1992). Microtubule dynamic instability and GTP hydrolysis. Annu. Rev. Biophys. Biomol. Struct. 21, 145-166.
16. Friedman P.A., and Platzer, E.G. (1978). Interaction of antihelmintic benzimidazoles and benzimidazole derivatives with bovine brain tubulin. Biochim. Biophys. Acta 544, 605-614.
17. Gliksman, N., Parsons, S.F., and Salmon, E.D. (1992). Okadaic acid induces interphase to mitotic-like microtubule dynamic instability by inactivating rescue. J. Cell Biol. 119, 1271-1276.
18. Hoebeke, J., Van Nijen, G., and DeBrabander, M. (1976). Interactions of oncodazole (R 17934), a new anti-tumoral drug, with rat brain tubulin. Biochem. Biophys. Res. Commun. 69, 319-324.
19. Hyman, A., Drechsel, D., Kellogg, Salser, D., S., Sawin, K., Steffen, P., Wordeman, L., and Mitchison, T. (1991). Preparation of modified tubulins. Methods Enzymol. 196, 478-485.
20. Hyman, A.A., Salser, S., Drechsel, D.N., Unwin, N., and Mitchison, T.J. (1992). Role of GTP hydrolysis in microtubule dynamics: information from a slowly hydrolyzable analogue, GMPCPP. Mol. Biol. Cell 3, 1155-1167.
21. Inoue, S., and Salmon, E.D. (1995). Force generation by microtubule assembly/disassembly in mitosis and related movements. Mol. Biol. Cell 6, 1619-1640.
22. Ireland, C.M., Gull, K., Gutteridge, W.E., and Pogson, C.I. (1979). The interaction of benzimidazole carbamates with mammalian microtubule protein. Biochem. Pharmacol. 28, 2680-2682.
23. Jordan, M.A., Margolis, R.L., Himes, R.H., and Wilson, L. (1986). Identification of a distinct class of vinblastine binding sites on microtubules. J. Mol. Biol. 187, 61-73.
24. Jordan, M.A., Thrower, D., and Wilson, L. (1991). Mechanism of inhibition of cell proliferation by the Vinca alkaloids. Cancer Res. 51, 2212-2222.
25. Jordan, M.A., Thrower, D., and Wilson, L. (1992). Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles: implications for the role of microtubule dynamics in mitosis. J. Cell Sci. 102, 401-416.
26. Jordan M.A., Toso, R.J., Thrower, D., and Wilson, L. (1993). Mechanism of mitotic block and inhibition of cell proliferation by Taxol at low concentrations. Proc. Natl. Acad. Sci. USA 90, 9552-9556.
27. Jordan, M.A., and Wilson, L. (1990). Kinetic analysis of tubulin exchange at microtubule ends at low vinblastine concentrations. Biochemistry 29, 2730-2739.
28. Lee, J.C., Field, D.J., and Lee, L.L.Y. (1980). Effects of nocodazole on the structures of calf brain tubulin. Biochemistry 19, 6209-6215.
29. Lin, CM., and Hamel, E. (1981). Effects of inhibitors of tubulin polymerization on GTP hydrolysis. J. Biol. Chem. 256, 9242-9245.
30. Mejillano, M.R., Shivanna, B.D., and Himes, R.H. (1995). The GT-Pase activity of tubulin. Mol. Biol. Cell 6, 258a (Abstract).
31. Melki, R., Carlier, M.F., and Pantaloni, D. (1990). Direct evidence for GTP and GDP-Pi intermediates in microtubule assembly. Biochemistry 29, 8921-8932.
32. Mitchison, T., and Kirschner, M.W. (1984a). Microtubule assembly nucleated by isolated centrosomes. Nature 312, 232-237.
33. Mitchison, T., and Kirschner, M. (1984b). Dynamic instability of microtubule growth. Nature 312, 237-242.
34. Morrisey, J.H. (1981). Silver stain for proteins in polyacrylanide gels: a modified procedure with enhanced sensitivity. Anal. Biochem. 117, 307-310.
35. Rose, G.G., Pomerat, C.M., Shindler, T.O., and Trunnell, J.B. (1958). A cellophane-strip technique for culturing tissue in multipurpose chambers. J. Biophys. Biochem. Cytol. 4, 761-764.
36. Shelden, E., and Wadsworth, P. (1993). Observation and quantification of individual microtubule behavior in vivo: microtubule dynamics are cell-type specific. J. Cell Biol. 120, 935-945.
37. Skoufias, D., and Wilson, L. (1992). Mechanism of inhibition of microtubule polymerization by colchicine: inhibitory potencies of unliganded colchicine and tubulin-colchicine complexes. Biochemistry 31, 738-746.
38. Tanaka, E., Ho, T., and Kirschner, M. (1995). The role of microtubule dynamics in growth cone motility and axonal growth. J. Cell Biol. 128, 139-155.
39. Taylor, J.R. (1982). An Introduction to Error Analysis, Mill Valley, CA: University Science Books, 207-217.
40. Toso, R.J., Jordan, M.A., Farrell, K.W., Matsumoto, B., and Wilson, L. (1993). Kinetic stabilization of microtubule dynamic instability in vitro by vinblastine. Biochemistry 32, 1285-1293.
41. Vasquez, R.J., Gard, D.L., and Cassimeris, L. (1994). XMAP from Xenopus eggs promote rapid plus end assembly of microtubules and rapid microtubule polymer turnover. J. Cell Biol. 127, 985-993.
42. Vandecandelaere, A., Martin, S.R., and Bayley, P.M. (1995). Regulation of microtubule dynamic instability by tubulin-GTP. Biochemistry 34, 1332-1343.
43. Vigers, G.P., Coue M., and McIntosh, J.R. (1988). Fluorescent microtubules break up under illumination. J. Cell Biol. 107, 1011-1024.
44. Wadsworth, P., and Salmon, E.D. (1986). Preparation and characterization of fluorescent analogs of tubulin. Methods Enzymol. 134, 519-528.
45. Walczak, C.E., Mitchison, T.J., and Desai, A. (1996). XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell 84, 37-47.
46. Walker, R.A., Inoue, S., and Salmon, E.D. (1989). Asymmetric behavior of severed microtubules following ultraviolet-microbeam irradiation of individual microtubules in vitro. J. Cell Biol. 108, 931-937.
47. Walker, R.A., O'Brien, E.T., Pryer, N.K., Soboeiro, M.F., Voter, W.A., Erickson, H.P., and Salmon, E.D. (1988). Dynamic instability of individual, MAP-free microtubules analyzed by video light microscopy: rate constants and transition frequencies. J. Cell Biol. 107, 1437-1448.
48. Walker, R.A., Pryer, N.K., and Salmon, E.D. (1991). Dilution of individual microtubules observed in real time in vitro: evidence that cap size is small and independent of elongation rate. J. Cell Biol. 114, 73-81.
49. Wendell, K.L, Wilson, L., and Jordan, M.A. (1993). Mitotic block in HeLa cells by vinblastine: ultrastructural changes in kinetochore-microtubule attachment and in centrosomes. J. Cell Sci. 104, 261-274.
50. Wilson, L., and Farrell, K.W. (1986). Kinetics and steady-state dynamics of tubulin addition and loss at opposite microtubule ends: the mechanism of action of colchicine. Ann. NY Acad. Sci. 466, 690-708.
51. Wilson, L., and Jordan, M.A. (1994). Pharmacological probes of microtubule function. In: Microtubules, ed. J.S. Hyams and C.W. Lloyd, New York: Wiley-Liss, 59-83.
52. Wilson, L., and Jordan, M.A. (1995). Microtubule dynamics: taking aim at a moving target. Chem. Biol. 2, 569-573.
53. Wilson, L., Jordan, M.A., Morse, A., and Margolis, R.L. (1982). Interaction of vinblastine with steady-state microtubules in vitro. J. Mol. Biol. 159, 129-149.