Abnormal centrosome amplification in the absence of p53

Science

Volumn 271, Issue 5256, 1996, Pages 1744-1747

  Fukasawa, Kenji ^a   Choi, Taesaeng ^a   Kuriyama, Ryoko ^b   Rulong, Shen ^a   Vande Woude, George F ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P53;

ANIMAL CELL; ARTICLE; CELL CYCLE; CENTROSOME; CHROMOSOME SEGREGATION; CONTROLLED STUDY; EMBRYO; FIBROBLAST; GENE AMPLIFICATION; GENETIC STABILITY; MITOSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; TUMOR SUPPRESSOR GENE;

ANIMALIA;

EID: 0029881977     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5256.1744     Document Type: Article

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61. -/- MEFs, incubation with anti-γ-tubulin antibody was followed by incubation with anti-γ-tubulin monoclonal antibody [TUB2-1 (Sigma)] for 1 hour The antibody-antigen complexes were detected with rhodamine-conjugated goat antibody to mouse IgG (Boerhinger Mannheim) for β-tubulin and fluorescein isothiocyanate (FITC)-conjugated goat antibody to rabit IgG (Boerhinger Mannheim) for γ-tubulin by incubation for 1 hour at room temperature Cells were washed extensively with PBS after each incubation Cells were then washed with trisbuffered saline and stained with DAPI
62. -/- MEFs; L A. Donehower, K. Vousden, and C Webb for helpful suggestions: and C. Rhoderick for secretarial assistance. Supported in part by the National Cancer Institute. Department of Health and Human Services (DHHS) with Advanced BioScience Laboratories, Incorporated, and the G Harold and Leila Y Mathers Chanta ble Foundation The contents of this publication do not necessarily reflect the views or policies of DHHS, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government.