Role of Lys-32 residues in R67 dihydrofolate reductase probed by asymmetric mutations

Journal of Biological Chemistry

Volumn 279, Issue 45, 2004, Pages 46995-47002

  Hicks, Stephanie N ^a   Smiley, R Derike ^a   Stinnett, Lori G ^a   Minor, Kenneth H ^a   Howell, Elizabeth E ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; CATALYSIS; CHEMICAL BONDS; GENES; PHOSPHATES; PROTEINS;

HOMOTETRAMERIC ENZYMES; LIGANDS; NON-PRODUCTIVE BINDING;

MUTAGENESIS;

AMINO ACID; DIHYDROFOLATE REDUCTASE; LYSINE;

ARTICLE; ASYMMETRIC CATALYSIS; GENE MUTATION; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL;

BINDING SITES; CATALYSIS; CIRCULAR DICHROISM; DIMERIZATION; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; IONS; KINETICS; LIGANDS; LYSINE; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MUTATION; NADP; PROTEIN BINDING; PROTEIN CONFORMATION; SALTS; SPECTROMETRY, FLUORESCENCE; TEMPERATURE; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 8744294941     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M404484200     Document Type: Article

References (46)

1. Narayana, N., Matthews, D. A., Howell, E. E., and Xuong, N. H. (1995) Nat. Struct. Biol. 2, 1018-1025
2. Bradrick, T. D., Beechem, J. M., and Howell, E. E. (1996) Biochemistry 35, 11414-11424
3. Howell, E. E., Shukla, U., Hicks, S. N., Smiley, R. D., Kuhn, L. A., and Zavodszky, M. I. (2001) J. Comput. Aided Mol. Des. 15, 1035-1052
4. Strader, M. B., Smiley, R. D., Stinnett, L. G., VerBerkmoes, N. C., and Howell, E. E. (2001) Biochemistry 40, 11344-11352
5. Bradrick, T. D., Shattuck, C., Strader, M. B., Wicker, C., Eisenstein, E., and Howell, E. E. (1996) J. Biol. Chem. 271, 28031-28037
6. Smiley, R. D., Stinnett, L. G., Saxton, A. M., and Howell, E. E. (2002) Biochemistry 41, 15664-15675
7. Hicks, S. N., Smiley, R. D., Hamilton, J. B., and Howell, E. E. (2003) Biochemistry 42, 10569-10578
8. Park, H., Bradrick, T. D., and Howell, E. E. (1997) Protein Eng. 10, 1415-1424
9. Strader, M. B., Chopra, S., Jackson, M., Smiley, R. D., Stinnett, L. G., Wu, J., and Howell, E. E. (2004) Biochemistry 43, 7403-7412
10. Hamilton, J. B. (1997) The Effect of a K32A Mutation on R67 Dihydrofolate Reductase. M.S. thesis in Biotechnology, University of Tennessee, Knoxville, TN
11. Park, C., and Raines, R. T. (2001) J. Am. Chem. Soc. 123, 11472-11479
12. Record, M. T., Jr., Lohman, T. M., and De Haseth, P. (1976) J. Mol. Biol. 107, 145-158
13. Pitcher, W. H., 3rd, DeRose, E. F., Mueller, G. A., Howell, E. E., and London, R. E. (2003) Biochemistry 42, 11150-11160
14. Strader, M. B., and Howell, E. E. (1997) Gibco-BRL Focus 19, 24-25
15. de Bernandez Clark, E., Schwartz, E., and Rudolph, R. (1999) Methods Enzymol. 309, 217-235
16. Nichols, R., Weaver, C. D., Eisenstein, E., Blakley, R. L., Appleman, J., Huang, T. H., Huang, F. Y., and Howell, E. E. (1993) Biochemistry 32, 1695-1706
17. West, F. W., Seo, H. S., Bradrick, T. D., and Howell, E. E. (2000) Biochemistry 39, 3678-3689
18. Royer, C. A., Mann, C. J., and Matthews, C. R. (1993) Protein Sci. 2, 1844-1852
19. Howell, E. E., Warren, M. S., Booth, C. L., Villafranca, J. E., and Kraut, J. (1987) Biochemistry 26, 8591-8598
20. Ellis, K. J., and Morrison, J. F. (1982) Methods Enzymol. 87, 405-426
21. Baccanari, D., Phillips, A., Smith, S., Sinski, D., and Burchall, J. (1975) Biochemistry 14, 5267-5273
22. Blakley, R. L. (1960) Nature 188, 231-232
23. Horecker, B. L., and Kornberg, A. (1948) J. Biol. Chem. 175, 385-390
24. Zhuang, P., Yin, M., Holland, J. C., Peterson, C. B., and Howell, E. E. (1993) J. Biol. Chem. 268, 22672-22679
25. Dunn, S. M., Lanigan, T. M., and Howell, E. E. (1990) Biochemistry 29, 8569-8576
26. Schuck, P. (2000) Biophys. J. 78, 1606-1619
27. Reece, L. J., Nichols, R., Ogden, R. C., and Howell, E. E. (1991) Biochemistry 30, 10895-10904
28. Woody, R. W. (1995) Methods Enzymol. 246, 34-71
29. Li, D., Levy, L. A., Gabel, S. A., Lebetkin, M. S., DeRose, E. F., Wall, M. J., Howell, E. E., and London, R. E. (2001) Biochemistry 40, 4242-4252
30. Jensen, D. E., and von Hippel, P. H. (1976) J. Biol. Chem. 251, 7198-7214
31. von Hippel, P. H., and Berg, O. G. (1989) J. Biol. Chem. 264, 675-678
32. Fersht, A. (1999) Structure and Mechanism in Protein Science, 2nd Ed., pp. 109-111, W. H. Freeman and Co., New York
33. Dougherty, R. C., and Howard, L. N. (2003) Biophys. Chem. 105, 269-278
34. Bagnol, L., Horner, J. H., and Newcomb, M. (2003) Org. Lett. 5, 5055-5058
35. Harder, S., Feil, F., and Repo, T. (2002) Chemistry 8, 1991-1999
36. Roca, M., Marti, S., Andres, J., Moliner, V., Tunon, I., Bertran, J., and Williams, I. H. (2003) J. Am. Chem. Soc. 125, 7726-7737
37. Umezurike, G. M. (1987) Biochem. J. 241, 455-462
38. Chong, L. T., Dempster, S. E., Hendsch, L. P., and Tidor, B. (1998) Protein Sci. 7, 206-210
39. Garel, L., Lozach, B., Dutasta, J., and Collet, A. (1993) J. Am. Chem. Soc. 115, 11652-11653
40. Schneider, H., Blatter, T., and Simova, S. (1991) J. Am. Chem. Soc. 113, 1996-2000
41. Houk, K. N., Leach, A. G., Kim, S. P., and Zhang, X. (2003) Angew. Chem. Int. Ed. Engl. 42, 4872-4897
42. Kuntz, I. D., Blaney, J. M., Oatley, S. J., Langridge, R., and Ferrin, T. E. (1982) J. Mol. Biol. 161, 269-288
43. Shoichet, B. K., and Kuntz, I. D. (1993) Protein Eng. 6, 723-732
44. Brito, R. M., Reddick, R., Bennett, G. N., Rudolph, F. B., and Rosevear, P. R. (1990) Biochemistry 29, 9825-9831
45. Brito, R. M., Rudolph, F. B., and Rosevear, P. R. (1991) Biochemistry 30, 1461-1469
46. Stinnett, L. G., Smiley, R. D., Hicks, S. N., and Howell, E. E. (2004) J. Biol. Chem. 279, 47003-47009