Engineered CRISPR-Cas9 nuclease with expanded targeting space

Science

Volumn 361, Issue 6408, 2018, Pages 1259-1262

  Nishimasu, Hiroshi ^a   Shi, Xi ^b,c   Ishiguro, Soh ^d,e   Gao, Linyi ^b,f   Hirano, Seiichi ^a   Okazaki, Sae ^a   Noda, Taichi ^g   Abudayyeh, Omar O ^b,c,h   Gootenberg, Jonathan S ^b,c,h   Mori, Hideto ^d,e   Oura, Seiya ^g,i   Holmes, Benjamin ^b,c   Tanaka, Mamoru ^d   Seki, Motoaki ^d   Hirano, Hisato ^a   Aburatani, Hiroyuki ^d   Ishitani, Ryuichiro ^a   Ikawa, Masahito ^a,g,i   Yachie, Nozomu ^a,d,e   Zhang, Feng ^b,c,f,h   Nureki, Osamu ^a   more..

^a UNIVERSITY OF TOKYO   (Japan)

^b BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d UNIVERSITY OF TOKYO   (Japan)

^e KEIO UNIVERSITY   (Japan)

^f Massachusetts Institute of Technology Cambridge   (United States)

^g OSAKA UNIVERSITY   (Japan)

^h MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

ⁱ OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CRISPR ASSOCIATED PROTEIN; CYTIDINE DEAMINASE; NUCLEIC ACID BASE; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; ENDONUCLEASE;

BIOENGINEERING; CELL; CRYSTAL STRUCTURE; DNA; GENE; GENE EXPRESSION; GENOMICS;

ARTICLE; CONTROLLED STUDY; CRISPR-CAS9 SYSTEM; CRYSTAL STRUCTURE; ENZYME ACTIVATION; GENE MUTATION; GENE TARGETING; GENETIC ENGINEERING; GENETIC VARIABILITY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEPLETION; PROTEIN DNA INTERACTION; PROTEIN MOTIF; PROTEIN TARGETING; WILD TYPE; CHEMISTRY; CRISPR CAS SYSTEM; GENE EDITING; GENETICS; HEK293 CELL LINE; PROTEIN ENGINEERING; X RAY CRYSTALLOGRAPHY;

STREPTOCOCCUS PYOGENES;

BACTERIAL PROTEINS; CRISPR-CAS SYSTEMS; CRYSTALLOGRAPHY, X-RAY; ENDONUCLEASES; GENE EDITING; HEK293 CELLS; HUMANS; PROTEIN ENGINEERING;

EID: 85054018722     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aas9129     Document Type: Article

References (16)

1. M. Jinek et al., Science 337, 816–821 (2012).
2. L. Cong et al., Science 339, 819–823 (2013).
3. S. H. Sternberg, S. Redding, M. Jinek, E. C. Greene, J. A. Doudna, Nature 507, 62–67 (2014).
4. B. P. Kleinstiver et al., Nature 523, 481–485 (2015).
5. F. A. Ran et al., Nature 520, 186–191 (2015).
6. B. Zetsche et al., Cell 163, 759–771 (2015).
7. C. Anders, O. Niewoehner, A. Duerst, M. Jinek, Nature 513, 569–573 (2014).
8. C. Anders, K. Bargsten, M. Jinek, Mol. Cell 61, 895–902 (2016).
9. S. Hirano, H. Nishimasu, R. Ishitani, O. Nureki, Mol. Cell 61, 886–894 (2016).
10. L. Gao et al., Nat. Biotechnol. 35, 789–792 (2017).
11. J. H. Hu et al., Nature 556, 57–63 (2018).
12. F. Jiang et al., Science 351, 867–871 (2016).
13. S. Q. Tsai et al., Nat. Biotechnol. 33, 187–197 (2015).
14. I. M. Slaymaker et al., Science 351, 84–88 (2016).
15. A. C. Komor, Y. B. Kim, M. S. Packer, J. A. Zuris, D. R. Liu, Nature 533, 420–424 (2016).
16. K. Nishida et al., Science 353, aaf8729 (2016).