Short DNA sequences inserted for gene targeting can accidentally interfere with off-target gene expression

FASEB Journal

Volumn 24, Issue 6, 2010, Pages 1714-1724

  Meier, Ingo D ^a   Bernreuther, Christian ^a   Tilling, Thomas ^a   Neidhardt, John ^a,b   Wong, Yong Wee ^a   Schulze, Christian ^a   Streichert, Thomas ^a   Schachner, Melitta ^a,c,d,e  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c RUTGERS UNIVERSITY   (United States)

^d RUTGERS UNIVERSITY   (United States)

^e Shantou Medical College   (China)

Author keywords

Conditional knockout mice; Genomic positional effects; Loxp sites

Indexed keywords

CELL PROTEIN; MESSENGER RNA; OLIGONUCLEOTIDE; PROTEIN U44; SMALL NUCLEOLAR RNA; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CENTRAL NERVOUS SYSTEM FUNCTION; DNA SEQUENCE; GAS5 GENE; GENE; GENE EXPRESSION; GENE INSERTION; GENE LOCUS; GENE TARGETING; GENOME; GENOMICS; INTRON; LOXP SITE; MICROARRAY ANALYSIS; MOUSE; NCAM GENE; NEO GENE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; TNR GENE;

ANIMALS; ANTIGENS, CD56; BASE SEQUENCE; BIOLOGICAL MARKERS; BLOTTING, NORTHERN; BLOTTING, WESTERN; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE TARGETING; GENETIC VECTORS; INTEGRASES; MICE; MICE, KNOCKOUT; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL NUCLEOLAR; TENASCIN;

ANIMALIA; MUS;

EID: 77953511814     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.09-140749     Document Type: Article

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