Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions

Nature Protocols

Volumn 7, Issue 11, 2012, Pages 2029-2040

  Beers, Jeanette ^a   Gulbranson, Daniel R ^b,c   George, Nicole ^d   Siniscalchi, Lauren I ^a   Jones, Jeffrey ^c,d   Thomson, James A ^b,c   Chen, Guokai ^a,b  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^b MORGRIDGE INSTITUTE FOR RESEARCH   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

^d WiCell Institute   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EDETIC ACID;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL TRANSPORT; COLONY FORMATION; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; DISSOCIATION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; STEM CELL EXPANSION; TERATOMA;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COLLAGEN; CULTURE MEDIA; DRUG COMBINATIONS; EDETIC ACID; FEMALE; HUMANS; LAMININ; MICE; MICE, SCID; PLURIPOTENT STEM CELLS; PROTEOGLYCANS;

EID: 84868135412     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2012.130     Document Type: Article

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