-
2
-
-
84920828713
-
DREADDs (designer receptors exclusively activated by designer drugs): Chemogenetic tools with therapeutic utility
-
Urban DJ, Roth BL (2015) DREADDs (designer receptors exclusively activated by designer drugs): Chemogenetic tools with therapeutic utility. Annu Rev Pharmacol Toxicol 55:399-417.
-
(2015)
Annu Rev Pharmacol Toxicol
, vol.55
, pp. 399-417
-
-
Urban, D.J.1
Roth, B.L.2
-
3
-
-
79960159955
-
Optogenetics in neural systems
-
Yizhar O, Fenno LE, Davidson TJ, Mogri M, Deisseroth K (2011) Optogenetics in neural systems. Neuron 71(1):9-34.
-
(2011)
Neuron
, vol.71
, Issue.1
, pp. 9-34
-
-
Yizhar, O.1
Fenno, L.E.2
Davidson, T.J.3
Mogri, M.4
Deisseroth, K.5
-
4
-
-
84455178930
-
The microbial opsin family of optogenetic tools
-
Zhang F, et al. (2011) The microbial opsin family of optogenetic tools. Cell 147(7): 1446-1457.
-
(2011)
Cell
, vol.147
, Issue.7
, pp. 1446-1457
-
-
Zhang, F.1
-
5
-
-
84877800939
-
Overexpression of membrane proteins in mammalian cells for structural studies
-
Andréll J, Tate CG (2013) Overexpression of membrane proteins in mammalian cells for structural studies. Mol Membr Biol 30(1):52-63.
-
(2013)
Mol Membr Biol
, vol.30
, Issue.1
, pp. 52-63
-
-
Andréll, J.1
Tate, C.G.2
-
6
-
-
84872356936
-
Protein engineering methods applied to membrane protein targets
-
Lluis MW, Godfroy JI, 3rd, Yin H (2013) Protein engineering methods applied to membrane protein targets. Protein Eng Des Sel 26(2):91-100.
-
(2013)
Protein Eng des Sel
, vol.26
, Issue.2
, pp. 91-100
-
-
Lluis, M.W.1
Godfroy, J.I.2
Yin, H.3
-
7
-
-
84923099296
-
Mechanisms of integral membrane protein insertion and folding
-
Cymer F, von Heijne G, White SH (2015) Mechanisms of integral membrane protein insertion and folding. J Mol Biol 427(5):999-1022.
-
(2015)
J Mol Biol
, vol.427
, Issue.5
, pp. 999-1022
-
-
Cymer, F.1
Von Heijne, G.2
White, S.H.3
-
8
-
-
0038819926
-
The chaperone environment at the cytoplasmic face of the endoplasmic reticulum can modulate rhodopsin processing and inclusion formation
-
Chapple JP, Cheetham ME (2003) The chaperone environment at the cytoplasmic face of the endoplasmic reticulum can modulate rhodopsin processing and inclusion formation. J Biol Chem 278(21):19087-19094.
-
(2003)
J Biol Chem
, vol.278
, Issue.21
, pp. 19087-19094
-
-
Chapple, J.P.1
Cheetham, M.E.2
-
9
-
-
77949325574
-
Trafficking of G-protein-coupled receptors to the plasma membrane: Insights for pharmacoperone drugs
-
Conn PM, Ulloa-Aguirre A (2010) Trafficking of G-protein-coupled receptors to the plasma membrane: Insights for pharmacoperone drugs. Trends Endocrinol Metab 21(3):190-197.
-
(2010)
Trends Endocrinol Metab
, vol.21
, Issue.3
, pp. 190-197
-
-
Conn, P.M.1
Ulloa-Aguirre, A.2
-
10
-
-
0036289574
-
Protein building blocks preserved by recombination
-
Voigt CA, Martinez C, Wang ZG, Mayo SL, Arnold FH (2002) Protein building blocks preserved by recombination. Nat Struct Biol 9(7):553-558.
-
(2002)
Nat Struct Biol
, vol.9
, Issue.7
, pp. 553-558
-
-
Voigt, C.A.1
Martinez, C.2
Wang, Z.G.3
Mayo, S.L.4
Arnold, F.H.5
-
11
-
-
0037436342
-
Archaeal-Type rhodopsins in Chlamydomonas: Model structure and intracellular localization
-
Suzuki T, et al. (2003) Archaeal-Type rhodopsins in Chlamydomonas: Model structure and intracellular localization. Biochem Biophys Res Commun 301(3):711-717.
-
(2003)
Biochem Biophys Res Commun
, vol.301
, Issue.3
, pp. 711-717
-
-
Suzuki, T.1
-
12
-
-
0037173051
-
Two rhodopsins mediate phototaxis to low-And high-intensity light in Chlamydomonas reinhardtii
-
Sineshchekov OA, Jung KH, Spudich JL (2002) Two rhodopsins mediate phototaxis to low-And high-intensity light in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 99(13):8689-8694.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, Issue.13
, pp. 8689-8694
-
-
Sineshchekov, O.A.1
Jung, K.H.2
Spudich, J.L.3
-
15
-
-
26444621497
-
Millisecond-Timescale, genetically targeted optical control of neural activity
-
Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K (2005) Millisecond-Timescale, genetically targeted optical control of neural activity. Nat Neurosci 8(9):1263-1268.
-
(2005)
Nat Neurosci
, vol.8
, Issue.9
, pp. 1263-1268
-
-
Boyden, E.S.1
Zhang, F.2
Bamberg, E.3
Nagel, G.4
Deisseroth, K.5
-
16
-
-
30944451943
-
Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels
-
Ishizuka T, Kakuda M, Araki R, Yawo H (2006) Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels. Neurosci Res 54(2):85-94.
-
(2006)
Neurosci Res
, vol.54
, Issue.2
, pp. 85-94
-
-
Ishizuka, T.1
Kakuda, M.2
Araki, R.3
Yawo, H.4
-
17
-
-
84878848170
-
Stabilizing membrane proteins through protein engineering
-
Scott DJ, Kummer L, Tremmel D, Plöckthun A (2013) Stabilizing membrane proteins through protein engineering. Curr Opin Chem Biol 17(3):427-435.
-
(2013)
Curr Opin Chem Biol
, vol.17
, Issue.3
, pp. 427-435
-
-
Scott, D.J.1
Kummer, L.2
Tremmel, D.3
Plöckthun, A.4
-
18
-
-
54449087770
-
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
-
Sarkar CA, et al. (2008) Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc Natl Acad Sci USA 105(39): 14808-14813.
-
(2008)
Proc Natl Acad Sci USA
, vol.105
, Issue.39
, pp. 14808-14813
-
-
Sarkar, C.A.1
-
19
-
-
35348832935
-
High-Throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae
-
Newstead S, Kim H, von Heijne G, Iwata S, Drew D (2007) High-Throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 104(35): 13936-13941.
-
(2007)
Proc Natl Acad Sci USA
, vol.104
, Issue.35
, pp. 13936-13941
-
-
Newstead, S.1
Kim, H.2
Von Heijne, G.3
Iwata, S.4
Drew, D.5
-
21
-
-
70450242805
-
Exploring protein fitness landscapes by directed evolution
-
Romero PA, Arnold FH (2009) Exploring protein fitness landscapes by directed evolution. Nat Rev Mol Cell Biol 10(12):866-876.
-
(2009)
Nat Rev Mol Cell Biol
, vol.10
, Issue.12
, pp. 866-876
-
-
Romero, P.A.1
Arnold, F.H.2
-
22
-
-
17244380498
-
On the conservative nature of intragenic recombination
-
Drummond DA, Silberg JJ, Meyer MM, Wilke CO, Arnold FH (2005) On the conservative nature of intragenic recombination. Proc Natl Acad Sci USA 102(15):5380-5385.
-
(2005)
Proc Natl Acad Sci USA
, vol.102
, Issue.15
, pp. 5380-5385
-
-
Drummond, D.A.1
Silberg, J.J.2
Meyer, M.M.3
Wilke, C.O.4
Arnold, F.H.5
-
23
-
-
9644291746
-
Site-directed protein recombination as a shortest-path problem
-
Endelman JB, Silberg JJ, Wang ZG, Arnold FH (2004) Site-directed protein recombination as a shortest-path problem. Protein Eng Des Sel 17(7):589-594.
-
(2004)
Protein Eng des Sel
, vol.17
, Issue.7
, pp. 589-594
-
-
Endelman, J.B.1
Silberg, J.J.2
Wang, Z.G.3
Arnold, F.H.4
-
24
-
-
84874094783
-
Chimeragenesis of distantly-related proteins by noncontiguous recombination
-
Smith MA, Romero PA, Wu T, Brustad EM, Arnold FH (2013) Chimeragenesis of distantly-related proteins by noncontiguous recombination. Protein Sci 22(2): 231-238.
-
(2013)
Protein Sci
, vol.22
, Issue.2
, pp. 231-238
-
-
Smith, M.A.1
Romero, P.A.2
Wu, T.3
Brustad, E.M.4
Arnold, F.H.5
-
25
-
-
33845624902
-
Structure-guided SCHEMA recombination of distantly related beta-lactamases
-
Meyer MM, Hochrein L, Arnold FH (2006) Structure-guided SCHEMA recombination of distantly related beta-lactamases. Protein Eng Des Sel 19(12):563-570.
-
(2006)
Protein Eng des Sel
, vol.19
, Issue.12
, pp. 563-570
-
-
Meyer, M.M.1
Hochrein, L.2
Arnold, F.H.3
-
26
-
-
0032518266
-
DNA shuffling of a family of genes from diverse species accelerates directed evolution
-
Crameri A, Raillard SA, Bermudez E, Stemmer WP (1998) DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 391(6664):288-291.
-
(1998)
Nature
, vol.391
, Issue.6664
, pp. 288-291
-
-
Crameri, A.1
Raillard, S.A.2
Bermudez, E.3
Stemmer, W.P.4
-
27
-
-
33646753362
-
Structure-guided recombination creates an artificial family of cytochromes P450
-
Otey CR, et al. (2006) Structure-guided recombination creates an artificial family of cytochromes P450. PLoS Biol 4(5):e112.
-
(2006)
PLoS Biol
, vol.4
, Issue.5
, pp. e112
-
-
Otey, C.R.1
-
28
-
-
34948815009
-
A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
-
Li Y, et al. (2007) A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments. Nat Biotechnol 25(9):1051-1056.
-
(2007)
Nat Biotechnol
, vol.25
, Issue.9
, pp. 1051-1056
-
-
Li, Y.1
-
29
-
-
84868117425
-
SCHEMA-designed variants of human arginase i and II reveal sequence elements important to stability and catalysis
-
Romero PA, et al. (2012) SCHEMA-designed variants of human arginase I and II reveal sequence elements important to stability and catalysis. ACS Synth Biol 1(6):221-228.
-
(2012)
ACS Synth Biol
, vol.1
, Issue.6
, pp. 221-228
-
-
Romero, P.A.1
-
30
-
-
65249175725
-
A family of thermostable fungal cellulases created by structure-guided recombination
-
Heinzelman P, et al. (2009) A family of thermostable fungal cellulases created by structure-guided recombination. Proc Natl Acad Sci USA 106(14):5610-5615.
-
(2009)
Proc Natl Acad Sci USA
, vol.106
, Issue.14
, pp. 5610-5615
-
-
Heinzelman, P.1
-
31
-
-
0037189362
-
Channelrhodopsin-1: A light-gated proton channel in green algae
-
Nagel G, et al. (2002) Channelrhodopsin-1: A light-gated proton channel in green algae. Science 296(5577):2395-2398.
-
(2002)
Science
, vol.296
, Issue.5577
, pp. 2395-2398
-
-
Nagel, G.1
-
32
-
-
0345133280
-
Channelrhodopsin-2, a directly light-gated cation-selective membrane channel
-
Nagel G, et al. (2003) Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc Natl Acad Sci USA 100(24):13940-13945.
-
(2003)
Proc Natl Acad Sci USA
, vol.100
, Issue.24
, pp. 13940-13945
-
-
Nagel, G.1
-
33
-
-
44349120639
-
Red-shifted optogenetic excitation: A tool for fast neural control derived from Volvox carteri
-
Zhang F, et al. (2008) Red-shifted optogenetic excitation: A tool for fast neural control derived from Volvox carteri. Nat Neurosci 11(6):631-633.
-
(2008)
Nat Neurosci
, vol.11
, Issue.6
, pp. 631-633
-
-
Zhang, F.1
-
34
-
-
70349199876
-
Channelrhodopsins of Volvox carteri are photochromic proteins that are specifically expressed in somatic cells under control of light, temperature, and the sex inducer
-
Kianianmomeni A, Stehfest K, Nematollahi G, Hegemann P, Hallmann A (2009) Channelrhodopsins of Volvox carteri are photochromic proteins that are specifically expressed in somatic cells under control of light, temperature, and the sex inducer. Plant Physiol 151(1):347-366.
-
(2009)
Plant Physiol
, vol.151
, Issue.1
, pp. 347-366
-
-
Kianianmomeni, A.1
Stehfest, K.2
Nematollahi, G.3
Hegemann, P.4
Hallmann, A.5
-
35
-
-
38349092685
-
Photoactivation of channelrhodopsin
-
Ernst OP, et al. (2008) Photoactivation of channelrhodopsin. J Biol Chem 283(3): 1637-1643.
-
(2008)
J Biol Chem
, vol.283
, Issue.3
, pp. 1637-1643
-
-
Ernst, O.P.1
-
36
-
-
84855190225
-
New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride
-
Govorunova EG, Spudich EN, Lane CE, Sineshchekov OA, Spudich JL (2011) New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride. MBio 2(3):e00115-11.
-
(2011)
MBio
, vol.2
, Issue.3
, pp. e00115-e00211
-
-
Govorunova, E.G.1
Spudich, E.N.2
Lane, C.E.3
Sineshchekov, O.A.4
Spudich, J.L.5
-
37
-
-
84855360168
-
Diversity of Chlamydomonas channelrhodopsins
-
Hou SY, et al. (2012) Diversity of Chlamydomonas channelrhodopsins. Photochem Photobiol 88(1):119-128.
-
(2012)
Photochem Photobiol
, vol.88
, Issue.1
, pp. 119-128
-
-
Hou, S.Y.1
-
38
-
-
84885605921
-
Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis
-
Govorunova EG, Sineshchekov OA, Li H, Janz R, Spudich JL (2013) Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis. J Biol Chem 288(41):29911-29922.
-
(2013)
J Biol Chem
, vol.288
, Issue.41
, pp. 29911-29922
-
-
Govorunova, E.G.1
Sineshchekov, O.A.2
Li, H.3
Janz, R.4
Spudich, J.L.5
-
39
-
-
84895524488
-
Independent optical excitation of distinct neural populations
-
Klapoetke NC, et al. (2014) Independent optical excitation of distinct neural populations. Nat Methods 11(3):338-346.
-
(2014)
Nat Methods
, vol.11
, Issue.3
, pp. 338-346
-
-
Klapoetke, N.C.1
-
40
-
-
84862777498
-
Crystal structure of the channelrhodopsin light-gated cation channel
-
Kato HE, et al. (2012) Crystal structure of the channelrhodopsin light-gated cation channel. Nature 482(7385):369-374.
-
(2012)
Nature
, vol.482
, Issue.7385
, pp. 369-374
-
-
Kato, H.E.1
-
41
-
-
84905281576
-
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
-
Hochbaum DR, et al. (2014) All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins. Nat Methods 11(8):825-833.
-
(2014)
Nat Methods
, vol.11
, Issue.8
, pp. 825-833
-
-
Hochbaum, D.R.1
-
42
-
-
77950930288
-
Molecular and cellular approaches for diversifying and extending optogenetics
-
Gradinaru V, et al. (2010) Molecular and cellular approaches for diversifying and extending optogenetics. Cell 141(1):154-165.
-
(2010)
Cell
, vol.141
, Issue.1
, pp. 154-165
-
-
Gradinaru, V.1
-
43
-
-
84870812948
-
A monomeric red fluorescent protein with low cytotoxicity
-
Shemiakina II, et al. (2012) A monomeric red fluorescent protein with low cytotoxicity. Nat Commun 3:1204.
-
(2012)
Nat Commun
, vol.3
, pp. 1204
-
-
Shemiakina, I.I.1
-
44
-
-
84939653879
-
Genetically encoded spy peptide fusion system to detect plasma membrane-localized proteins in vivo
-
Bedbrook CN, et al. (2015) Genetically encoded spy peptide fusion system to detect plasma membrane-localized proteins in vivo. Chem Biol 22(8):1108-1121.
-
(2015)
Chem Biol
, vol.22
, Issue.8
, pp. 1108-1121
-
-
Bedbrook, C.N.1
-
45
-
-
84858679420
-
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
-
Zakeri B, et al. (2012) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci USA 109(12):E690-E697.
-
(2012)
Proc Natl Acad Sci USA
, vol.109
, Issue.12
, pp. E690-E697
-
-
Zakeri, B.1
-
47
-
-
33745894174
-
Rationalizing membrane protein overexpression
-
Wagner S, Bader ML, Drew D, de Gier JW (2006) Rationalizing membrane protein overexpression. Trends Biotechnol 24(8):364-371.
-
(2006)
Trends Biotechnol
, vol.24
, Issue.8
, pp. 364-371
-
-
Wagner, S.1
Bader, M.L.2
Drew, D.3
De Gier, J.W.4
-
48
-
-
58849124558
-
Bi-stable neural state switches
-
Berndt A, Yizhar O, Gunaydin LA, Hegemann P, Deisseroth K (2009) Bi-stable neural state switches. Nat Neurosci 12(2):229-234.
-
(2009)
Nat Neurosci
, vol.12
, Issue.2
, pp. 229-234
-
-
Berndt, A.1
Yizhar, O.2
Gunaydin, L.A.3
Hegemann, P.4
Deisseroth, K.5
-
49
-
-
84890957779
-
Hypocrea jecorina cellobiohydrolase i stabilizing mutations identified using noncontiguous recombination
-
Smith MA, Bedbrook CN, Wu T, Arnold FH (2013) Hypocrea jecorina cellobiohydrolase I stabilizing mutations identified using noncontiguous recombination. ACS Synth Biol 2(12):690-696.
-
(2013)
ACS Synth Biol
, vol.2
, Issue.12
, pp. 690-696
-
-
Smith, M.A.1
Bedbrook, C.N.2
Wu, T.3
Arnold, F.H.4
-
50
-
-
70350351556
-
SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability
-
Heinzelman P, et al. (2009) SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability. J Biol Chem 284(39): 26229-26233.
-
(2009)
J Biol Chem
, vol.284
, Issue.39
, pp. 26229-26233
-
-
Heinzelman, P.1
-
51
-
-
84906975620
-
Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons
-
Flytzanis NC, et al. (2014) Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons. Nat Commun 5:4894.
-
(2014)
Nat Commun
, vol.5
, pp. 4894
-
-
Flytzanis, N.C.1
-
52
-
-
84879546356
-
Silico platform for prediction of N-, O-And C-glycosites in eukaryotic protein sequences
-
Chauhan JS, Rao A, Raghava GP (2013) In silico platform for prediction of N-, O-And C-glycosites in eukaryotic protein sequences. PLoS One 8(6):e67008.
-
(2013)
PLoS One
, vol.8
, Issue.6
, pp. e67008
-
-
Chauhan, J.S.1
Rao, A.2
Raghava, G.P.3
-
53
-
-
84925884361
-
Designing libraries of chimeric proteins using SCHEMA recombination and RASPP
-
Smith MA, Arnold FH (2014) Designing libraries of chimeric proteins using SCHEMA recombination and RASPP. Methods Mol Biol 1179:335-343.
-
(2014)
Methods Mol Biol
, vol.1179
, pp. 335-343
-
-
Smith, M.A.1
Arnold, F.H.2
-
54
-
-
84925884326
-
Noncontiguous SCHEMA protein recombination
-
Smith MA, Arnold FH (2014) Noncontiguous SCHEMA protein recombination. Methods Mol Biol 1179:345-352.
-
(2014)
Methods Mol Biol
, vol.1179
, pp. 345-352
-
-
Smith, M.A.1
Arnold, F.H.2
-
55
-
-
33845792555
-
CellProfiler: Image analysis software for identifying and quantifying cell phenotypes
-
Carpenter AE, et al. (2006) CellProfiler: Image analysis software for identifying and quantifying cell phenotypes. Genome Biol 7(10):R100.
-
(2006)
Genome Biol
, vol.7
, Issue.10
, pp. R100
-
-
Carpenter, A.E.1
-
56
-
-
79952595565
-
The NumPy array: A structure for efficient numerical computation
-
Walt SVD, Colbert SC, Varoquaux G (2011) The NumPy array: A structure for efficient numerical computation. Comput Sci Eng 13(2):22-30.
-
(2011)
Comput Sci Eng
, vol.13
, Issue.2
, pp. 22-30
-
-
Svd, W.1
Colbert, S.C.2
Varoquaux, G.3
-
57
-
-
34247500374
-
Python for scientific computing
-
Oliphant TE (2007) Python for scientific computing. Comput Sci Eng 9(3):10-20.
-
(2007)
Comput Sci Eng
, vol.9
, Issue.3
, pp. 10-20
-
-
Oliphant, T.E.1
-
58
-
-
34247493236
-
Matplotlib: A 2D graphics environment
-
Hunter JD (2007) Matplotlib: A 2D graphics environment. Comput Sci Eng 9(3):90-95.
-
(2007)
Comput Sci Eng
, vol.9
, Issue.3
, pp. 90-95
-
-
Hunter, J.D.1
|