Noncontiguous SCHEMA protein recombination

Methods in Molecular Biology

Volumn 1179, Issue , 2014, Pages 345-352

  Smith, Matthew A ^a   Arnold, Frances H ^b  

^a Insight Data Science   (United States)

^b California Institute of Technology   (United States)

Author keywords

Chimeragenesis; Homologous recombination; NCR; Noncontiguous recombination; Protein engineering; SCHEMA

Indexed keywords

CELLULOSE 1,4 BETA CELLOBIOSIDASE; CHIMERIC PROTEIN; PEPTIDE LIBRARY; RECOMBINANT PROTEIN;

ALGORITHM; AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CHAETOMIUM; CHAETOMIUM THERMOPHILUM; CHIMERA; DISULFIDE BOND; ENZYME ACTIVE SITE; HOMOLOGOUS RECOMBINATION; HYPOCREA JECORINA; MOLECULAR CLONING; MOLECULAR LIBRARY; NONCONTIGUOUS RECOMBINATION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; SCHEMA METHOD; SEQUENCE ALIGNMENT; TALAROMYCES; TALAROMYCES EMERSONII; DIRECTED MOLECULAR EVOLUTION; GENETICS; METABOLISM; PEPTIDE LIBRARY; PROCEDURES; PROTEIN ENGINEERING;

ALGORITHMS; DIRECTED MOLECULAR EVOLUTION; PEPTIDE LIBRARY; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 84925884326     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1053-3_23     Document Type: Article

References (12)

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9. Li Y, Drummond DA, Sawayama AM, Snow CD, Bloom JD, Arnold FH (2007) A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments. Nat Biotechnol 25:1051-1056
10. Krause A (2010) SFO: a toolbox for submodular function optimization. J Mach Learn Res 11:1141-1144
11. Romero P, Stone E, Lamb C, Chantranupong L, Krause A, Miklos A, Hughes R, Fechtel B, Ellington AD, Arnold FH, Georgiou G (2012) SCHEMA-designed variants of human Arginase I and II reveal sequence elements important to stability and catalysis. ACS Synth Biol 1:221-228
12. Heinzelman P, Komor R, Kanaan A, Romero PA, Yu X, Mohler S, Snow C, Arnold FH (2010) Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination. Protein Eng Des Sel 23:871-880