Designing libraries of chimeric proteins using SCHEMA recombination and RASPP

Methods in Molecular Biology

Volumn 1179, Issue , 2014, Pages 335-343

  Smith, Matthew A ^a   Arnold, Frances H ^b  

^a Insight Data Science   (United States)

^b California Institute of Technology   (United States)

Author keywords

Chimeragenesis; Homologous recombination; Protein engineering; RASPP; SCHEMA

Indexed keywords

CELLULOSE 1,4 BETA CELLOBIOSIDASE; CHIMERIC PROTEIN; PEPTIDE LIBRARY;

ALGORITHM; AMINO ACID SEQUENCE; ARTICLE; NONHUMAN; PEPTIDE LIBRARY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; RASPP ALGORITHM; SCHEMA LIBRARY; SEQUENCE ALIGNMENT; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; PROCEDURES; PROTEIN ENGINEERING;

ALGORITHMS; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; PEPTIDE LIBRARY; PROTEIN ENGINEERING;

EID: 84925884361     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1053-3_22     Document Type: Article

References (12)

1. Voigt CA, Martinez C, Wang Z-G, Mayo SL, Arnold FH (2002) Protein building blocks preserved by recombination. Nat Struct Biol 9:553-558
2. Meyer M, Hochrein L, Arnold FH (2006) Structure-guided SCHEMA recombination of distantly related ?-lactamases. Protein Eng Des Sel 19:563-570
3. Endelman J, Silberg J, Wang Z, Arnold FH (2004) Site-directed protein recombination as a shortest-path problem. Protein Eng Des Sel 17:589-594
4. Romero P, Stone E, Lamb C, Chantranupong L, Krause A, Miklos A, Hughes R, Fechtel B, Ellington AD, Arnold FH, Georgiou G (2012) SCHEMA-designed variants of human Arginase I and II reveal sequence elements important to stability and catalysis. ACS Synth Biol 1:221-228
5. Li Y, Drummond DA, Sawayama AM, Snow CD, Bloom JD, Arnold FH (2007) A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments. Nat Biotechnol 25:1051-1056
6. Heinzelman P, Komor R, Kanaan A, Romero PA, Yu X, Mohler S, Snow C, Arnold FH (2010) Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination. Protein Eng Des Sel 23: 871-880
7. Otey CR, Landwehr M, Endelman JB, Hiraga K, Bloom JD, Arnold FH (2006) Structureguided recombination creates an artificial family of cytochromes P450. PLoS Biol 4:e112
8. Heinzelman P, Romero PA, Arnold FH (2013) Efficient sampling of SCHEMA chimera families for identification of useful sequence elements. In: Keasling A (ed) Methods in enzymology: methods in protein design. Elsevier Ltd, Oxford, UK
9. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948
10. Eswar N, Webb B, Marti-Renom MA, Madhusudhan MS, Eramian D, Shen M-Y, Pieper U, Sali A (2007) Comparative protein structure modeling using MODELLER. Curr Protoc Protein Sci 50:291-2931
11. Hiraga K, Arnold FH (2003) General method for sequence-independent site-directed chimeragenesis. J Mol Biol 330:287-296
12. Farrow MF, Arnold FH (2010) Combinatorial recombination of gene fragments to construct a library of chimeras. Curr Protoc Protein Sci 62:2621-26220