Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons

Nature Communications

Volumn 5, Issue , 2014, Pages

  Flytzanis, Nicholas C ^a   Bedbrook, Claire N ^b   Chiu, Hui ^a   Engqvist, Martin K M ^a   Xiao, Cheng ^b   Chan, Ken Y ^b   Sternberg, Paul W ^b   Arnold, Frances H ^a   Gradinaru, Viviana ^a,b  

^a California Institute of Technology HHMI   (United States)

^b California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARCHAERHODOPSIN 3; PROTEIN VARIANT; RHODOPSIN; UNCLASSIFIED DRUG; ARCHAEAL PROTEIN; ARCHAERHODOPSIN PROTEIN, ARCHAEA; HELMINTH PROTEIN; ISOPROTEIN; NERVE PROTEIN; RECOMBINANT PROTEIN;

FLUORESCENCE; INHIBITION; LIGHT INTENSITY; MAMMAL; NEMATODE; NEUROLOGY; PROBE; SENSOR; WAVELENGTH;

ACTION POTENTIAL; ANIMAL CELL; ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; ELECTRIC POTENTIAL; EVOKED SOMATOSENSORY RESPONSE; FLUORESCENCE; GREEN LIGHT; IN VIVO STUDY; KINETICS; LIGHT; LIGHT INTENSITY; MUTANT; NERVE CELL; NERVE CELL CULTURE; NERVE CELL NETWORK; NONHUMAN; OPTOGENETICS; RED LIGHT; WILD TYPE; ANIMAL; CHEMISTRY; GENE EXPRESSION; GENETICS; HIPPOCAMPUS; METABOLISM; PATCH CLAMP TECHNIQUE; PHYSIOLOGY; PRIMARY CELL CULTURE; PROCEDURES; RAT; SPECTROFLUOROMETRY; WISTAR RAT;

CAENORHABDITIS ELEGANS; MAMMALIA;

ACTION POTENTIALS; ANIMALS; ARCHAEAL PROTEINS; CAENORHABDITIS ELEGANS; GENE EXPRESSION; HELMINTH PROTEINS; HIPPOCAMPUS; LIGHT; NERVE TISSUE PROTEINS; NEURONS; OPTOGENETICS; PATCH-CLAMP TECHNIQUES; PRIMARY CELL CULTURE; PROTEIN ISOFORMS; RATS; RATS, WISTAR; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 84906975620     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms5894     Document Type: Article

References (31)

1. Gobel, W., Kampa, B. M. &Helmchen, F. Imaging cellular network dynamics in three dimensions using fast 3D laser scanning. Nat. Methods 4, 73-79 (2007)
2. Cao, G. et al. Genetically targeted optical electrophysiology in intact neural circuits. Cell 154, 904-913 (2013)
3. Chen, T. W. et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499, 295-300 (2013)
4. Peters, A. J., Chen, S. X. &Komiyama, T. Emergence of reproducible spatiotemporal activity during motor learning. Nature 510, 263-267 (2014)
5. Quirin, S., Jackson, J., Peterka, D. S. &Yuste, R. Simultaneous imaging of neural activity in three dimensions. Front. Neural Circuits 8, 29 (2014)
6. Mutoh, H., Akemann, W. &Knopfel, T. Genetically engineered fluorescent voltage reporters. ACS Chem Neurosci 3, 585-592 (2012)
7. St-Pierre, F. et al. High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor. Nat. Neurosci. 17, 884-889 (2014)
8. Gong, Y., Wagner, M. J., Zhong, Li, J. &Schnitzer, M. J. Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors. Nat. Commun. 5, 3674 (2014)
9. Hochbaum, D. R. et al. All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins. Nat. Methods 11, 825-833 (2014)
10. Tian, L. et al. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat. Methods 6, 875-881 (2009)
11. Akerboom, J. et al. Optimization of a GCaMP calcium indicator for neural activity imaging. J. Neurosci. 32, 13819-13840 (2012)
12. Jin, L. et al. Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron 75, 779-785 (2012)
13. Mattis, J. et al. Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat. Methods 9, 159-172 (2012)
14. Lin, J. Y., Knutsen, P. M., Muller, A., Kleinfeld, D. &Tsien, R. Y. ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Nat. Neurosci. 16, 1499-1508 (2013)
15. Klapoetke, N. C. et al. Independent optical excitation of distinct neural populations. Nat. Methods 11, 338-346 (2014)
16. Chow, B. Y. et al. High-performance genetically targetable optical neural silencing by light-driven proton pumps. Nature 463, 98-102 (2010)
17. Kralj, J. M., Douglass, A. D., Hochbaum, D. R., Maclaurin, D. &Cohen, A. E. Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Nat. Methods 9, 90-95 (2012)
18. Gong, Y., Li, J. Z. &Schnitzer, M. J. Enhanced Archaerhodopsin fluorescent protein voltage indicators. PLoS ONE 8, e66959 (2013)
19. Engqvist, M. K. et al. Directed Evolution of Gloeobacter violaceus Rhodopsin Spectral Properties. J. Mol. Biol. doi:10.1016/j.jmb.2014.06.015 (2014)
20. McIsaac, R. S. et al. Directed evolution of a far-red fluorescent rhodopsin. Proc. Natl Acad. Sci. doi:10.1073/pnas.1413987111 (2014)
21. Gradinaru, V. et al. Molecular and cellular approaches for diversifying and extending optogenetics. Cell 141, 154-165 (2010)
22. Chalasani, S. H. et al. Dissecting a circuit for olfactory behaviour in Caenorhabditis elegans. Nature 450, 63-70 (2007)
23. Kato, S., Xu, Y., Cho, C. E., Abbott, L. F. &Bargmann, C. I. Temporal responses of C. elegans chemosensory neurons are preserved in behavioral dynamics. Neuron 81, 616-628 (2014)
24. Nickell, W. T., Pun, R. Y., Bargmann, C. I. &Kleene, S. J. Single ionic channels of two Caenorhabditis elegans chemosensory neurons in native membrane. J. Membr. Biol. 189, 55-66 (2002)
25. Gao, S. &Zhen, M. Action potentials drive body wall muscle contractions in Caenorhabditis elegans. Proc. Natl Acad. Sci. USA 108, 2557-2562 (2011)
26. Looger, L. L. Running in reverse: rhodopsins sense voltage. Nat. Methods 9, 43-44 (2012)
27. Grishkevich, V., Hashimshony, T. &Yanai, I. Core promoter T-blocks correlate with gene expression levels in C. elegans. Genome Res. 21, 707-717 (2011)
28. Mello, C. &Fire, A. DNA transformation. Methods Cell. Biol. 48, 451-482 (1995)
29. Okazaki, A., Sudo, Y. &Takagi, S. Optical silencing of C. elegans cells with arch proton pump. PLoS ONE 7, e35370 (2012)
30. Chronis, N., Zimmer, M. &Bargmann, C. I. Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans. Nat. Methods 4, 727-731 (2007)
31. Cho, J. Y. &Sternberg, P. W. Multilevel modulation of a sensory motor circuit during C. elegans sleep and arousal. Cell 156, 249-260 (2014)