A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids

Stem Cell Reports

Volumn 10, Issue 1, 2018, Pages 314-328

  Takahashi, Yu ^a,b   Sato, Shintaro ^a,d   Kurashima, Yosuke ^a   Yamamoto, Tomohisa ^a,b   Kurokawa, Shiho ^a   Yuki, Yoshikazu ^a   Takemura, Naoki ^a   Uematsu, Satoshi ^a   Lai, Chen Yi ^a   Otsu, Makoto ^a   Matsuno, Hiroshi ^c   Osawa, Hideki ^c   Mizushima, Tsunekazu ^c   Nishimura, Junichi ^c   Hayashi, Mikio ^b   Yamaguchi, Takayuki ^b   Kiyono, Hiroshi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b JAPAN TOBACCO INC   (Japan)

^c OSAKA UNIVERSITY   (Japan)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

conditioned medium; epithelial organoids; human iPSCs; IECs; IESCs; intestinal organoids; lentiviral infection; organoid differentiation; suspension culture

Indexed keywords

APOLIPOPROTEIN B; APOLIPOPROTEIN C3; CARCINOEMBRYONIC ANTIGEN RELATED CELL ADHESION MOLECULE 7; CHEMOKINE RECEPTOR CXCR4; FIBROBLAST GROWTH FACTOR 2; GLYCOPROTEIN; GLYCOPROTEIN A33; HEPATOCYTE NUCLEAR FACTOR 3BETA; INTERLEUKIN 22; LENTIVIRUS VECTOR; LYSOZYME; MUCIN 2; NOG PROTEIN; RSPO1 PROTEIN; TRANSCRIPTION FACTOR CDX2; TRANSCRIPTION FACTOR SOX17; UNCLASSIFIED DRUG; VILLIN; VILLIN 1; WNT PROTEIN; WNT3A PROTEIN; WNT3A PROTEIN, HUMAN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CHO CELL LINE; COLON; CONTROLLED STUDY; DNA MICROARRAY; ENDODERM; ENTEROENDOCRINE CELL; FEMALE; GENE EXPRESSION; GENETIC TRANSDUCTION; GOBLET CELL; HEK293T CELL LINE; HIGH THROUGHPUT SCREENING; HOST CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; INDUCED PLURIPOTENT STEM CELL; INTESTINE EPITHELIUM CELL; LENTIVIRUS INFECTION; LUCIFERASE ASSAY; MOUSE; NCTC CLONE 929 CELL LINE; NONHUMAN; ORGANOID; PANETH CELL; PRIORITY JOURNAL; SMALL INTESTINE; STABLE EXPRESSION; SUSPENSION CELL CULTURE; WESTERN BLOTTING; WNT SIGNALING; ANIMAL; CELL CULTURE TECHNIQUE; CYTOLOGY; EPITHELIUM CELL; GENE VECTOR; GENETICS; INTESTINE MUCOSA; LENTIVIRUS; METABOLISM; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; EPITHELIAL CELLS; GENETIC VECTORS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; INTESTINAL MUCOSA; LENTIVIRUS; MICE; ORGANOIDS; WNT3A PROTEIN;

EID: 85042780502     PISSN: 22136711     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.stemcr.2017.11.004     Document Type: Article

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