Author Correction: Efficient genetic engineering of human intestinal organoids using electroporation (Nature Protocols, (2015), 10, 10, (1474-1485), 10.1038/nprot.2015.088);Efficient genetic engineering of human intestinal organoids using electroporation

Nature Protocols

Volumn 10, Issue 10, 2015, Pages 1474-1485

  Fujii, Masayuki ^a,b   Matano, Mami ^a   Nanki, Kosaku ^a   Sato, Toshiro ^a  

^a KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GROWTH FACTOR;

ARTICLE; BASEMENT MEMBRANE; CELL CULTURE; CONTROLLED STUDY; CULTURE TECHNIQUE; ELECTROPORATION; FRAMESHIFT MUTATION; GENE EXPRESSION; GENE TARGETING; GENE TRANSFER; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; NONHUMAN; PRIORITY JOURNAL; TRANSPOSON; ANIMAL; CELL CULTURE TECHNIQUE; CELL LINE; COMPARATIVE STUDY; CYTOLOGY; CYTOPLASM; FLOW CYTOMETRY; INTESTINE; MOUSE; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; ELECTROPORATION; FLOW CYTOMETRY; GENETIC ENGINEERING; HUMANS; INTESTINES; MICE; ORGANOIDS;

EID: 84942524980     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/s41596-018-0107-5     Document Type: Erratum

References (11)

1. Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262-265 (2009).
2. Sato, T. et al. Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. Gastroenterology 141, 1762-1772 (2011).
3. Koo, B. K. et al. Controlled gene expression in primary Lgr5 organoid cultures. Nat. Methods 9, 81-83 (2012).
4. Schwank, G. et al. Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Cell Stem Cell 13, 653-658 (2013).
5. Matano, M. et al. Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. Nat. Med. 21, 256-262 (2015).
6. Wang, F. et al. Isolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay. Gastroenterology 145, 383-395 (2013).
7. Melkonyan, H., Sorg, C. & Klempt, M. Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO). Nucleic Acids Res. 24, 4356-4357 (1996).
8. Ding, S. et al. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell 122, 473-483 (2005).
9. Drost, J. et al. Sequential cancer mutations in cultured human intestinal stem cells. Nature 521, 43-47 (2015).
10. Ran, F. A. et al. Genome engineering using the CRISPR-Cas9 system. Nat. Protoc. 8, 2281-2308 (2013).
11. Schwank, G., Andersson-Rolf, A., Koo, B. K., Sasaki, N. & Clevers, H. Generation of BAC transgenic epithelial organoids. PLoS ONE 8, e76871 (2013).