Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope

Enzyme and Microbial Technology

Volumn 113, Issue , 2018, Pages 1-8

  Porter, Joanne L ^a   Sabatini, Selina ^a   Manning, Jack ^a   Tavanti, Michele ^a   Galman, James L ^a   Turner, Nicholas J ^a   Flitsch, Sabine L ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Biocatalysis; C H activation; CYP116B; Cytochrome P450 monooxygenase; Demethylation; Halomonas sp

Indexed keywords

ACTIVATION ANALYSIS; ESCHERICHIA COLI; HYDROXYLATION; OXIDATION;

BIOCATALYSIS; C-H ACTIVATION; CYP116B; DEMETHYLATION; HALOMONAS SP; MONOOXYGENASES;

CLONING;

CYTOCHROME C; CYTOCHROME P450; CYTOCHROME P450 116B62; CYTOCHROME P450 FAMILY 11; CYTOCHROME P450 HAL1; GLUCOSE; GLUCOSE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SULFATE;

ARTICLE; BIOCATALYST; CELL LYSATE; COBETIA MARINA; DNA DEMETHYLATION; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENOME ANALYSIS; HALOMONAS; HALOMONAS AQUAMARINA; HALOMONAS AXIALENSIS; HALOMONAS CUPIDA; HALOMONAS HYDROTHERMALIS; HALOMONAS MERIDIANA; HALOMONAS NEPTUNIA; HALOMONAS SULFIDAERIS; HYDROXYLATION; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SULFOXIDATION; BIOCATALYSIS; CHEMISTRY; DEMETHYLATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYLOGENY; PROCEDURES;

BIOCATALYSIS; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DEMETHYLATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HALOMONAS; HYDROXYLATION; NADP; PHYLOGENY; SUBSTRATE SPECIFICITY; SULFATES;

EID: 85042385736     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2018.02.005     Document Type: Article

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