Engineering cytochrome P450 monooxygenase CYP 116B3 for high dealkylation activity

Biotechnology Letters

Volumn 32, Issue 6, 2010, Pages 841-845

  Liu, Luo ^a   Schmid, Rolf D ^a   Urlacher, Vlada B ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Cytochrome P450; Dealkylation; Directed evolution; High throughput screening

Indexed keywords

7-ETHOXYCOUMARIN; CYTOCHROME P450; DEALKYLATION; DEMETHYLATION; DIRECTED EVOLUTION; HIGH-THROUGHPUT SCREENING; HIGH-THROUGHPUT SCREENING SYSTEMS; MONOOXYGENASES; RECOMBINANT E. COLI; SITE-SPECIFIC MUTAGENESIS;

ESCHERICHIA COLI;

7 ETHOXYCOUMARIN; 7-ETHOXYCOUMARIN; COUMARIN DERIVATIVE; CYTOCHROME P450; MIXED FUNCTION OXIDASE; RECOMBINANT PROTEIN;

ARTICLE; DEALKYLATION; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; PROTEIN ENGINEERING; RHODOCOCCUS; SITE DIRECTED MUTAGENESIS;

COUMARINS; CYTOCHROME P-450 ENZYME SYSTEM; DEALKYLATION; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; MIXED FUNCTION OXYGENASES; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RHODOCOCCUS;

ESCHERICHIA COLI;

EID: 77952240604     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0233-9     Document Type: Article

References (14)

1. Arnold FH, Georgiou G (2003) Directed evolution library creation: methods and protocols. Humana Press Inc., Totowa, New Jersey.
2. Cryle MJ, Stok JE, De Voss JL (2003) Reactions catalyzed by bacterial cytochromes P450. Aust J Chem 56: 749-762.
3. Farinas ET, Schwaneberg U, Glieder A, Arnold FH (2001) Directed evolution of a cytochrome P450 monooxygenase for alkane oxidation. Adv Synth Catal 343: 601-606.
4. Liu L, Schmid RD, Urlacher VB (2006) Cloning, expression, and characterization of a self-sufficient cytochrome P450 monooxygenase from Rhodococcus ruber DSM 44319. Appl Microbiol Biotechnol 72: 876-882.
5. Mansuy D (1994) Cytochromes P450 and model systems-great diversity of catalyzed reactions. Pure Appl Chem 66: 737-744.
6. Munro AW, Leys DG, McLean KJ, Marshall KR, Ost TWB, Daff S, Miles CS, Chapman SK, Lysek DA, Moser CC, Page CC, Dutton PL (2002) P450BM3: the very model of a modern flavocytochrome. Trends Biochem Sci 27: 250-257.
7. Nelson DR (2009) The cytochrome P450 homepage. Hum Genomics 4: 59-65.
8. Olsen M, Iverson B, Georgiou G (2000) High-throughput screening of enzyme libraries. Curr Opin Biotechnol 11: 331-337.
9. Omura T, Sato RJ (1964) The carbon monoxide-binding pigment of liver microsomes. J Biol Chem 239: 2370-2378.
10. Otey CR, Bandara G, Lalonde J, Takahashi K, Arnold FH (2006) Preparation of human metabolites of propranolol using laboratory-evolved bacterial cytochromes P450. Biotechnol Bioeng 93: 494-499.
11. Sono M, Roach MP, Coulter ED, Dawson JH (1996) Heme-containing oxygenases. Chem Rev 96: 2841-2888.
12. Urlacher VB, Eiben S (2006) Cytochrome P450 monooxygenases: perspectives for synthetic application. Trends Biotechnol 24: 324-330.
13. Urlacher VB, Makhsumkhanov A, Schmid RD (2006) Biotransformation of beta-ionone by engineered cytochrome P450 BM-3. Appl Microbiol Biotechnol 70: 53-59.
14. Wong TS, Arnold FH, Schwaneberg U (2004) Laboratory evolution of cytochrome P450BM-3 monooxygenase for organic cosolvents. Biotechnol Bioeng 85: 351-358.