Engineering of recombinant E. coli cells co-expressing P450pyrTM monooxygenase and glucose dehydrogenase for highly regio- and stereoselective hydroxylation of alicycles with cofactor recycling

Biotechnology and Bioengineering

Volumn 110, Issue 2, 2013, Pages 363-373

  Pham, Son Q ^a   Gao, Pengfei ^a   Li, Zhi ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Cofactor regeneration; Enantioselectivity; Hydroxylation; P450 monooxygenase; Regioselectivity; Whole cell biocatalysis

Indexed keywords

CARBON ATOMS; CO-FACTOR RECYCLING; COFACTOR REGENERATION; E. COLI; FERREDOXIN REDUCTASE; GLUCOSE DEHYDROGENASE; HIGH ACTIVITY; MONOOXYGENASES; PHARMACEUTICAL INTERMEDIATES; PRODUCTIVE SYSTEMS; RECOMBINANT E. COLI; RESTING CELLS; STEREO-SELECTIVE; STEREOSELECTIVE HYDROXYLATIONS; TRIPLE MUTANTS; WHOLE-CELL BIOCATALYSIS; WILD TYPES;

ACTIVATED CARBON; CELL ENGINEERING; ENANTIOSELECTIVITY; ENZYME ACTIVITY; GENES; GLUCOSE; HYDROXYLATION; PRODUCTIVITY; RECYCLING; REGIOSELECTIVITY; STEREOSELECTIVITY;

ESCHERICHIA COLI;

CARBON; FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; GLUCOSE; GLUCOSE DEHYDROGENASE; N BENZYLPYRROLIDINE; PYRROLIDINE DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BIOCATALYST; ESCHERICHIA COLI; HYDROXYLATION; NONHUMAN; STEREOCHEMISTRY; WILD TYPE;

BACTERIAL PROTEINS; CELL PROLIFERATION; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FERREDOXIN-NADP REDUCTASE; FERREDOXINS; GLUCOSE 1-DEHYDROGENASE; HYDROXYLATION; NADP; PROTEIN ENGINEERING; PYRROLIDINES; RECOMBINANT FUSION PROTEINS; STEREOISOMERISM;

EID: 84871847408     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24632     Document Type: Article

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