CRISPR-Cas9 toolkit for actinomycete genome editing

Methods in Molecular Biology

Volumn 1671, Issue , 2018, Pages 163-184

  Tong, Yaojun ^a   Robertsen, Helene Lunde ^a   Blin, Kai ^a   Weber, Tilmann ^a   Lee, Sang Yup ^a,b  

^a Chalmers University of Technology   (Denmark)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Actinomycetes; CRISPR Cas9; CRISPRi; Double strand break (DSB); Genome editing; Homology directed repair (HDR); Non homologous end joining (NHEJ); Synthetic biology; Uracil specific excision reagent (USER) cloning

Indexed keywords

URACIL;

ACTINOBACTERIA; CLONING; CRISPR-CAS9 SYSTEM; GENE CLUSTER; GENE EDITING; GENE LOSS; METABOLIC ENGINEERING; NONHUMAN; SOFTWARE; STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES COLLINUS; BACTERIAL GENOME; BIOLOGY; CRISPR CAS SYSTEM; DNA REPAIR; DOUBLE STRANDED DNA BREAK; GENE KNOCKDOWN; GENE VECTOR; GENETICS; GENOMICS; LOSS OF FUNCTION MUTATION; MOLECULAR CLONING; MULTIGENE FAMILY; PROCEDURES;

ACTINOBACTERIA; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; DNA REPAIR; GENE EDITING; GENE KNOCKDOWN TECHNIQUES; GENETIC VECTORS; GENOME, BACTERIAL; GENOMICS; LOSS OF FUNCTION MUTATION; MULTIGENE FAMILY; SOFTWARE;

EID: 85035101520     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-7295-1_11     Document Type: Chapter

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