Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

Applied Microbiology and Biotechnology

Volumn 99, Issue 24, 2015, Pages 10575-10585

  Zeng, Hu ^a   Wen, Shishi ^a   Xu, Wei ^a   He, Zhaoren ^a   Zhai, Guifa ^a   Liu, Yunkun ^a   Deng, Zixin ^a   Sun, Yuhui ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

ActI ORF2; Actinorhodin biosynthesis; CodA; CRISPR Cas9; Gene editing

Indexed keywords

ANTIBIOTICS; BACTERIA; BIOCHEMISTRY; BIOSYNTHESIS; CHAIN LENGTH; CHAINS; KETONES;

ACTI-ORF2; CODA; COMBINED SYSTEM; CRISPR/CAS9; DRUG-RESISTANT BACTERIA; LABORATORY TECHNIQUES; STREPTOMYCES COELICOLOR; WHOLE GENOME SEQUENCING;

GENES;

ACTINORHODINE; CASPASE 9; CYTOSINE DEAMINASE; FUNGAL DNA; POLYKETIDE; ANTHRAQUINONE DERIVATIVE; ANTIINFECTIVE AGENT;

ANTIBIOTICS; BACTERIUM; BIOTECHNOLOGY; DRUG RESISTANCE; ENZYME ACTIVITY; GENE EXPRESSION; GENOME; PROTEIN;

ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; FUNGAL GENE; GENE DELETION; GENOME; HOMOLOGOUS RECOMBINATION; MUTANT; NONHUMAN; PLASMID; PROGENY; STREPTOMYCES COELICOLOR; GENETIC RECOMBINATION; GENETIC SELECTION; GENETICS; METABOLIC ENGINEERING; METABOLISM; MUTATION;

ACTINOBACTERIA (CLASS); STREPTOMYCES; STREPTOMYCES COELICOLOR;

ANTHRAQUINONES; ANTI-BACTERIAL AGENTS; CRISPR-CAS SYSTEMS; METABOLIC ENGINEERING; MUTATION; POLYKETIDES; RECOMBINATION, GENETIC; SELECTION, GENETIC; STREPTOMYCES COELICOLOR;

EID: 84948382257     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6931-4     Document Type: Article

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