Software-supported user cloning strategies for site-directed mutagenesis and DNA assembly

ACS Synthetic Biology

Volumn 4, Issue 3, 2015, Pages 342-349

  Genee, Hans Jasper ^a   Bonde, Mads Tvillinggaard ^a   Bagger, Frederik Otzen ^b,c,d   Jespersen, Jakob Berg ^e   Sommer, Morten O A ^a,e   Wernersson, Rasmus ^e,f   Olsen, Lars Rønn ^b,d  

^a Chalmers University of Technology   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c FINSEN LABORATORY   (Denmark)

^d UNIVERSITY OF COPENHAGEN   (Denmark)

^e TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^f INTOMICS A S   (Denmark)

Author keywords

DNA assembly; point mutation; primer design; site directed mutagenesis; USER cloning; Web server

Indexed keywords

DNA; GREEN FLUORESCENT PROTEIN; YELLOW FLUORESCENT PROTEIN; PRIMER DNA;

ARTICLE; AUTOMATED MODIFICATION WITH USER CLONING; COMPUTER PROGRAM; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SITE DIRECTED MUTAGENESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; INTERNET; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POINT MUTATION; PROCEDURES; SYNTHETIC BIOLOGY;

BASE SEQUENCE; CLONING, MOLECULAR; DNA; DNA PRIMERS; ESCHERICHIA COLI; INTERNET; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; SOFTWARE; SYNTHETIC BIOLOGY;

EID: 84925639491     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb500194z     Document Type: Article

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