Evaluation of FASP, SP3, and iST Protocols for Proteomic Sample Preparation in the Low Microgram Range

Journal of Proteome Research

Volumn 16, Issue 11, 2017, Pages 4060-4072

  Sielaff, Malte ^a   Kuharev, Jörg ^a   Bohn, Toszka ^a   Hahlbrock, Jennifer ^a   Bopp, Tobias ^a   Tenzer, Stefan ^a   Distler, Ute ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

bottom up proteomics; filter aided sample preparation (FASP); in StageTip digestion (iST); sample preparation; single pot solid phase enhanced sample preparation (SP3); tumor associated macrophages (TAMs)

Indexed keywords

CARBOXYLIC ACID; PEPTIDE; PROTEIN; PROTEOME;

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BONE MARROW DERIVED MACROPHAGE; CELL FRACTIONATION; CELL LYSATE; CELL ORGANELLE; EVALUATION STUDY; HELA CELL LINE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOCOMPETENT CELL; INCUBATION TIME; MOUSE; NONHUMAN; PERFORMANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEOMICS; REPRODUCIBILITY; SAMPLE; SAMPLE SIZE; SOLID; TUMOR ASSOCIATED LEUKOCYTE; TUMOR BIOPSY; TUMOR MODEL; PROCEDURES; SPECIMEN HANDLING; STANDARDS; WORKFLOW;

HELA CELLS; HUMANS; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SAMPLE SIZE; SPECIMEN HANDLING; WORKFLOW;

EID: 85032830146     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/acs.jproteome.7b00433     Document Type: Article

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