Reproducible tissue homogenization and protein extraction for quantitative proteomics using micropestle-assisted pressure-cycling technology

Journal of Proteome Research

Volumn 15, Issue 6, 2016, Pages 1821-1829

  Shao, Shiying ^a,b   Guo, Tiannan ^a   Gross, Vera ^c   Lazarev, Alexander ^c   Koh, Ching Chiek ^a   Gillessen, Silke ^d   Joerger, Markus ^d   Jochum, Wolfram ^d   Aebersold, Ruedi ^a,e  

^a ETH ZURICH   (Switzerland)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c Pressure BioSciences   (United States)

^d CANTONAL HOSPITAL ST GALLEN   (Switzerland)

^e UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

mass spectrometry; PCT MicroCap; PCT MicroPestle; pressure cycling technology; SWATH

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOPSY; BRAIN TISSUE; CONTROLLED STUDY; CYTOLYSIS; EXTRACTION; HEART; HOMOGENATE; HUMAN; HUMAN TISSUE; KIDNEY PARENCHYMA; LIVER; MASS SPECTROMETRY; MOUSE; NONHUMAN; PRESSURE; PRESSURE CYCLING TECHNOLOGY; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEOMICS; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SEQUENTIAL WINDOWED ACQUISITION OF ALL THEORETICAL FRAGMENT ION MASS SPECTROMETRY; TECHNOLOGY; TISSUE HOMOGENIZATION; TISSUE PREPARATION; ANIMAL; DEVICES; ISOLATION AND PURIFICATION; PROCEDURES; WORKFLOW;

PROTEIN;

ANIMALS; BIOPSY; MICE; PRESSURE; PROTEINS; PROTEOMICS; REPRODUCIBILITY OF RESULTS; TECHNOLOGY; WORKFLOW;

EID: 84973544398     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/acs.jproteome.5b01136     Document Type: Article

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