Comparison of detergent-based sample preparation workflows for LTQ-Orbitrap analysis of the Escherichia coli proteome

Proteomics

Volumn 13, Issue 17, 2013, Pages 2597-2607

  Tanca, Alessandro ^a,b   Biosa, Grazia ^a   Pagnozzi, Daniela ^a   Addis, Maria Filippa ^a   Uzzau, Sergio ^a,b  

^a Porto Conte Ricerche Srl   (Italy)

^b UNIVERSITY OF SASSARI   (Italy)

Author keywords

FASP; Gram negative bacteria; Microbiology; RapiGest; SDS; Shotgun proteomics

Indexed keywords

BACTERIAL PROTEIN; DETERGENT; MEMBRANE PROTEIN; PEPTIDE; PROTEOME;

AMINO ACID SEQUENCE; ANALYTICAL EQUIPMENT; ARTICLE; CELLULAR DISTRIBUTION; COMPUTER PROGRAM; ESCHERICHIA COLI; EXTRACTION; FILTER; FILTER AIDED SAMPLE PREPARATION; GRAM NEGATIVE BACTERIUM; LIQUID CHROMATOGRAPHY; MASS SPECTROMETER; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEOMICS; SHOTGUN PROTEOMICS;

FASP; GRAM-NEGATIVE BACTERIA; MICROBIOLOGY; RAPIGEST; SDS; SHOTGUN PROTEOMICS;

CHROMATOGRAPHY, LIQUID; DETERGENTS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; PROTEOME; PROTEOMICS; SPECIMEN HANDLING; TANDEM MASS SPECTROMETRY; WORKFLOW;

EID: 84883295723     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201200478     Document Type: Article

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