Set of Novel Automated Quantitative Microproteomics Protocols for Small Sample Amounts and Its Application to Kidney Tissue Substructures

Journal of Proteome Research

Volumn 15, Issue 12, 2016, Pages 4722-4730

  De Graaf, Erik Leonardus ^a   Pellegrini, Davide ^a,b   McDonnell, Liam A ^a,c  

^a Fondazione Pisana per la Scienza ONLUS   (Italy)

^b SCUOLA NORMALE SUPERIORE   (Italy)

^c LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

automated; cortex; high pH fractionation; kidney proteome; label free; medulla; microgram sample amount; modified BCA assay; preconditioning; protein assay; TMT

Indexed keywords

PROTEOME;

ANIMAL TISSUE; ARTICLE; AUTOMATION; CELL LYSATE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; FEMALE; FRACTIONATION; HUMAN; HUMAN CELL; KIDNEY CORTEX; KIDNEY MEDULLA; LASER CAPTURE MICRODISSECTION; MICRO BCA PROTEIN ASSAY; MICRODISSECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN CONTENT; PROTEIN DETERMINATION; QUANTITATIVE ANALYSIS; TANDEM MASS SPECTROMETRY; TISSUE STRUCTURE; ANIMAL; CHEMISTRY; KIDNEY; PROCEDURES; PROTEOMICS; REPRODUCIBILITY; SAMPLE SIZE; STAINING; ULTRASTRUCTURE;

ANIMALS; KIDNEY; KIDNEY CORTEX; KIDNEY MEDULLA; LASER CAPTURE MICRODISSECTION; MICE; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SAMPLE SIZE; STAINING AND LABELING;

EID: 85000786916     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/acs.jproteome.6b00889     Document Type: Article

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