RNA editing with CRISPR-Cas13

Science

Volumn 358, Issue 6366, 2017, Pages 1019-1027

  Cox, David B T ^a,b,c   Gootenberg, Jonathan S ^a,b,c,d   Abudayyeh, Omar O ^a,b,c   Franklin, Brian ^a,b,c   Kellner, Max J ^a,b,c   Joung, Julia ^a,b,c   Zhang, Feng ^a,b,c  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c USA   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEAMINASE; GUIDE RNA; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; RNA; TRANSCRIPTOME; ADENOSINE DEAMINASE; BACTERIAL PROTEIN; ENDONUCLEASE; HYBRID PROTEIN;

BIOTECHNOLOGY; CATALYSIS; CELLS AND CELL COMPONENTS; DISEASE TREATMENT; ENZYME; ENZYME ACTIVITY; MUTATION; NUCLEIC ACID; PROTEIN; RESEARCH WORK; RNA;

ARTICLE; BIOTECHNOLOGY; CELLULAR DISTRIBUTION; CODON; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS13 SYSTEM; DNA STRAND; ENZYME SPECIFICITY; GENE EXPRESSION; GENE MUTATION; HEK293-FT CELL LINE; HUMAN; HUMAN CELL; LEPTOTRICHIA; LUCIFERASE ASSAY; MAMMAL CELL; MUTAGENESIS; NONHUMAN; NUCLEAR EXPORT; ORTHOLOGY; PORPHYROMONAS; PREVOTELLA; PRIORITY JOURNAL; PROTEIN ENGINEERING; RNA BINDING; RNA EDITING; RNA SEQUENCE; CLASSIFICATION; FANCONI ANEMIA; GENE KNOCKDOWN; GENE THERAPY; GENETICS; HEK293 CELL LINE; METABOLISM; NEPHROGENIC DIABETES INSIPIDUS; PROCEDURES;

MAMMALIA;

ADENOSINE DEAMINASE; BACTERIAL PROTEINS; BIOTECHNOLOGY; CRISPR-CAS SYSTEMS; DIABETES INSIPIDUS, NEPHROGENIC; ENDONUCLEASES; FANCONI ANEMIA; GENE KNOCKDOWN TECHNIQUES; GENETIC THERAPY; HEK293 CELLS; HUMANS; MUTAGENESIS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RNA EDITING;

EID: 85032331429     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aaq0180     Document Type: Article

References (32)

1. P. D. Hsu, E. S. Lander, F. Zhang, Cell 157, 1262–1278 (2014).
2. A. C. Komor, A. H. Badran, D. R. Liu, Cell 168, 20–36 (2017).
3. L. Cong et al., Science 339, 819–823 (2013).
4. P. Mali et al., Science 339, 823–826 (2013).
5. B. Zetsche et al., Cell 163, 759–771 (2015).
6. H. Kim, J. S. Kim, Nat. Rev. Genet. 15, 321–334 (2014).
7. A. C. Komor, Y. B. Kim, M. S. Packer, J. A. Zuris, D. R. Liu, Nature 533, 420–424 (2016).
8. K. Nishida et al., Science 353, aaf8729 (2016).
9. Y. B. Kim et al., Nat. Biotechnol. 35, 371–376 (2017).
10. O. O. Abudayyeh et al., Science 353, aaf5573 (2016).
11. S. Shmakov et al., Mol. Cell 60, 385–397 (2015).
12. S. Shmakov et al., Nat. Rev. Microbiol. 15, 169–182 (2017).
13. A. A. Smargon et al., Mol. Cell 65, 618–630.e7 (2017).
14. J. S. Gootenberg et al., Science 356, 438–442 (2017).
15. O. O. Abudayyeh et al., Nature 550, 280–284 (2017).
16. K. Nishikura, Annu. Rev. Biochem. 79, 321–349 (2010).
17. M. H. Tan et al., Nature 550, 249–254 (2017).
18. B. L. Bass, H. Weintraub, Cell 55, 1089–1098 (1988).
19. M. M. Matthews et al., Nat. Struct. Mol. Biol. 23, 426–433 (2016).
20. Y. Zheng, C. Lorenzo, P. A. Beal, Nucleic Acids Res. 45, 3369–3377 (2017).
21. A. Kuttan, B. L. Bass, Proc. Natl. Acad. Sci. U.S.A. 109, E3295–E3304 (2012).
22. S. K. Wong, S. Sato, D. W. Lazinski, RNA 7, 846–858 (2001).
23. M. Fukuda et al., Sci. Rep. 7, 41478 (2017).
24. M. F. Montiel-Gonzalez, I. Vallecillo-Viejo, G. A. Yudowski, J. J. Rosenthal, Proc. Natl. Acad. Sci. U.S.A. 110, 18285–18290 (2013).
25. M. F. Montiel-González, I. C. Vallecillo-Viejo, J. J. Rosenthal, Nucleic Acids Res. 44, e157 (2016).
26. J. Wettengel, P. Reautschnig, S. Geisler, P. J. Kahle, T. Stafforst, Nucleic Acids Res. 45, 2797–2808 (2017).
27. Y. Wang, J. Havel, P. A. Beal, ACS Chem. Biol. 10, 2512–2519 (2015).
28. K. A. Lehmann, B. L. Bass, Biochemistry 39, 12875–12884 (2000).
29. T. Stafforst, M. F. Schneider, Angew. Chem. Int. Ed. Engl. 51, 11166–11169 (2012).
30. C. Ballatore, V. M. Lee, J. Q. Trojanowski, Nat. Rev. Neurosci. 8, 663–672 (2007).
31. Y. Li et al., J. Invest. Dermatol. 130, 2768–2772 (2010).
32. R. C. Ferreira et al., Nat. Genet. 42, 777–780 (2010).