Nucleic acid detection with CRISPR-Cas13a/C2c2

Science

Volumn 356, Issue 6336, 2017, Pages 438-442

  Gootenberg, Jonathan S ^a,b,c,d,e   Abudayyeh, Omar O ^a,b,c,d   Lee, Jeong Wook ^f   Essletzbichler, Patrick ^a,b,c,d   Dy, Aaron J ^a,d   Joung, Julia ^a,b,c,d   Verdine, Vanessa ^a,b,c,d   Donghia, Nina ^f   Daringer, Nichole M ^d   Freije, Catherine A ^a,f   Myhrvold, Cameron ^a,f   Bhattacharyya, Roby P ^a   Livny, Jonathan ^a   Regev, Aviv ^a,c   Koonin, Eugene V ^g   Hung, Deborah T ^a   Sabeti, Pardis C ^a,f,h,i   Collins, James J ^a,c,d,f   Zhang, Feng ^a,b,c,d  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d Massachusetts Institute of Technology Cambridge   (United States)

^e HARVARD MEDICAL SCHOOL   (United States)

^f HARVARD UNIVERSITY   (United States)

^g NATIONAL INSTITUTES OF HEALTH   (United States)

^h HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

ⁱ HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; BACTERIAL DNA; BACTERIAL PROTEIN; CIRCULATING TUMOR DNA; RIBONUCLEASE; VIRUS RNA;

AMPLIFICATION; BACTERIUM; DETECTION METHOD; ENZYME ACTIVITY; GENE EXPRESSION; ISOTHERM; MOLECULAR ANALYSIS; MUTATION; NUCLEIC ACID; PROTEIN; TUMOR; VIRUS;

ARTICLE; CELL FREE SYSTEM; CRISPR CAS SYSTEM; DENGUE VIRUS; DNA FLANKING REGION; ESCHERICHIA COLI; GENOTYPE; KLEBSIELLA PNEUMONIAE; NONHUMAN; NUCLEIC ACID ANALYSIS; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SINGLE NUCLEOTIDE POLYMORPHISM; STORAGE; VIRUS DETECTION; VIRUS STRAIN; ZIKA VIRUS; BACTERIUM; CHEMISTRY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DENGUE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MUTATION; NEOPLASM; PATHOGENICITY; POINT OF CARE SYSTEM; RNA CLEAVAGE; ZIKA FEVER;

DENGUE VIRUS;

BACTERIA; BACTERIAL PROTEINS; CIRCULATING TUMOR DNA; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DENGUE; DENGUE VIRUS; DNA, BACTERIAL; HUMANS; MUTATION; NEOPLASMS; POINT-OF-CARE SYSTEMS; RIBONUCLEASES; RNA CLEAVAGE; RNA, VIRAL; ZIKA VIRUS; ZIKA VIRUS INFECTION;

EID: 85017652697     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aam9321     Document Type: Article

References (24)

1. K. Pardee et al., Cell 165, 1255-1266 (2016).
2. K. Pardee et al., Cell 159, 940-954 (2014).
3. A.A. Green, P. A. Silver, J. J. Collins, P. Yin, Cell 159, 925-939 (2014).
4. R. M. Kumar et al., Nature 516, 56-61 (2014).
5. M. Urdea et al., Nature 444 (suppl. 1), 73-79 (2006).
6. Y. Du et al., Angew. Chem. Int. Ed. 56, 992-996 (2017).
7. B. Zetsche et al., Cell 163, 759-771 (2015).
8. S. Shmakov et al., Nat. Rev. Microbiol. 15, 169-182 (2017).
9. S. Shmakov et al., Mol. Cell 60, 385-397 (2015).
10. O. O. Abudayyeh et al., Science 353, aaf5573 (2016).
11. A. A. Smargon et al., Mol. Cell 65, 618-630.e7 (2017).
12. A. East-Seletsky et al., Nature 538, 270-273 (2016).
13. R. Emmadi et al., J. Mol. Diagn. 13, 583-604 (2011).
14. L. Song et al., Anal. Chem. 85, 1932-1939 (2013).
15. J. M. Barletta et al., Am. J. Clin. Pathol. 122, 20-27 (2004).
16. J. Compton, Nature 350, 91-92 (1991).
17. O. Piepenburg et al., PLOS Biol. 4, e204 (2006).
18. W. Dejnirattisai et al., Nat. Immunol. 17, 1102-1108 (2016).
19. G. Paz-Bailey et al., N. Engl. J. Med. NEJMoa1613108 (2017).
20. N. Gupta et al., Clin. Infect. Dis. 53, 60-67 (2011).
21. N. Eriksson et al., PLOS Genet. 6, e1000993 (2010).
22. Z. Qin et al., Chin. J. Cancer 35, 36 (2016).
23. C. Bettegowda et al.., Sci. Transl. Med. 6, 224ra24 (2014).
24. A. M. Newman et al., Nat. Med. 20, 548-554 (2014).