Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling

Molecular Cell

Volumn 68, Issue 1, 2017, Pages 233-246.e5

  Michel, Martin A ^a   Swatek, Kirby N ^a   Hospenthal, Manuela K ^a,b   Komander, David ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

affimer; HUWE1; Lys6 linked ubiquitin chains; Mfn2; microscale thermophoresis; mitophagy; Parkin; X ray crystallography

Indexed keywords

BENZYLOXYCARBONYLLEUCYLLEUCYLLEUCINAL; MITOFUSIN 2; SHORT HAIRPIN RNA; UBIQUITIN; CARRIER PROTEIN; GUANOSINE TRIPHOSPHATASE; HUWE1 PROTEIN, HUMAN; LYSINE; MFN2 PROTEIN, HUMAN; MITOCHONDRIAL PROTEIN; MOLECULAR PROBE; PROTEIN BINDING; RECOMBINANT PROTEIN; RNF144A PROTEIN, HUMAN; RNF144B PROTEIN, HUMAN; UBIQUITIN PROTEIN LIGASE;

ARTICLE; BINDING SITE; CELL LYSATE; CONFOCAL MICROSCOPY; CONFORMATION; CONTROLLED STUDY; CROSS REACTION; CRYSTAL STRUCTURE; DEPOLARIZATION; DIMERIZATION; FLUORESCENCE MICROSCOPY; FLUORESCENCE POLARIZATION; HUMAN; HUMAN CELL; IN VITRO STUDY; ISOTHERMAL TITRATION CALORIMETRY; KINETICS; LIGHT SCATTERING; MASS SPECTROMETRY; NONHUMAN; PHAGE DISPLAY; PROTEOMICS; QUALITATIVE ANALYSIS; SIGNAL TRANSDUCTION; SIZE EXCLUSION CHROMATOGRAPHY; SURFACE PLASMON RESONANCE; UBIQUITINATION; ULTRAVIOLET RADIATION; WESTERN BLOTTING; CHEMISTRY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HEK293 CELL LINE; HELA CELL LINE; METABOLISM; MOLECULAR CLONING; MOLECULAR MODEL; MOLECULAR PROBE; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PROCESSING; PROTEIN SECONDARY STRUCTURE; TUMOR CELL LINE; X RAY CRYSTALLOGRAPHY;

AMINO ACID MOTIFS; BINDING SITES; CARRIER PROTEINS; CELL LINE, TUMOR; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GENE EXPRESSION; GTP PHOSPHOHYDROLASES; HEK293 CELLS; HELA CELLS; HUMANS; KINETICS; LYSINE; MITOCHONDRIAL PROTEINS; MODELS, MOLECULAR; MOLECULAR PROBES; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION; SUBSTRATE SPECIFICITY; UBIQUITIN; UBIQUITIN-PROTEIN LIGASES; UBIQUITINATION;

EID: 85029693774     PISSN: 10972765     EISSN: 10974164     Source Type: Journal    
DOI: 10.1016/j.molcel.2017.08.020     Document Type: Article

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