Laboratory evolution of a biotin-requiring Saccharomyces cerevisiae strain for full biotin prototrophy and identification of causal mutations

Applied and Environmental Microbiology

Volumn 83, Issue 16, 2017, Pages

  Bracher, Jasmine M ^a   de Hulster, Erik ^a   Koster, Charlotte C ^a   van den Broek, Marcel ^a   Daran, Jean Marc G ^a   van Maris, Antonius J A ^a,b   Pronk, Jack T ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Adaptive laboratory evolution; Biotin; Prototrophy; Reverse metabolic engineering; Saccharomyces cerevisiae; Vitamin biosynthesis; Whole genome sequencing

Indexed keywords

BATCH CELL CULTURE; BIOCHEMISTRY; BIOSYNTHESIS; CHROMOSOMES; GENE EXPRESSION; GENES; GROWTH RATE; LABORATORIES; METABOLIC ENGINEERING; METABOLISM; YEAST;

ADAPTIVE LABORATORY EVOLUTION; BIOTIN; CHROMOSOMAL REARRANGEMENT; INDEPENDENT EVOLUTIONS; PROTOTROPHY; REPEATED-BATCH CULTURE; SACCHAROMYCES CEREVISIAE STRAINS; WHOLE GENOME SEQUENCING;

COENZYMES;

BIOLOGICAL PRODUCTION; ENZYME ACTIVITY; EXPERIMENTAL STUDY; GENE; GENOME; GROWTH RATE; IDENTIFICATION METHOD; LABORATORY METHOD; MICROORGANISM; MOLECULAR ANALYSIS; MUTATION; VITAMIN; YEAST;

SACCHAROMYCES CEREVISIAE;

BIOTIN; SACCHAROMYCES CEREVISIAE PROTEIN;

FUNGAL GENOME; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MUTATION; SACCHAROMYCES CEREVISIAE;

BIOTIN; GENOME, FUNGAL; MUTATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 85026546145     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00892-17     Document Type: Article

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