Extraction of genomic DNA from yeasts for PCR-based applications

BioTechniques

Volumn 50, Issue 5, 2011, Pages 325-328

  Lõoke, Marko ^a   Kristjuhan, Kersti ^a   Kristjuhan, Arnold ^a  

^a UNIVERSITY OF TARTU   (Estonia)

Author keywords

DNA Extraction; PCR; Yeast

Indexed keywords

ALCOHOL; DNA FRAGMENT; FUNGAL DNA; GENOMIC DNA; LITHIUM ACETATE;

ARTICLE; CANDIDA ALBICANS; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; FUNGAL CELL CULTURE; KLUYVEROMYCES LACTIS; LYSIS; NONHUMAN; PICHIA PASTORIS; PRECIPITATION; REAL TIME POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; YEAST CELL;

ACETATES; CANDIDA ALBICANS; DNA, FUNGAL; KLUYVEROMYCES; PICHIA; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; SODIUM DODECYL SULFATE; YEASTS;

CANDIDA ALBICANS; KLUYVEROMYCES LACTIS; PICHIA ANGUSTA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 79957745171     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113672     Document Type: Article

References (7)

1. Ling, M., F. Merante, and B.H. Robinson. 1995. A rapid and reliable DNA preparation method for screening a large number of yeast clones by polymerase chain reaction. Nucleic Acids Res. 23:4924-4925.
2. Amberg, D.C., D.J. Burke, and J.N. Strathern. 2005. Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual. 2005 ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
3. Harju, S., H. Fedosyuk, and K.R. Peterson. 2004. Rapid isolation of yeast genomic DNA: Bust n' Grab. BMC Biotechnol. 4:8.
4. Akada, R., T. Murakane, and Y. Nishizawa. 2000. DNA extraction method for screening yeast clones by PCR. Biotechniques 28:668-670, 672, 674.
5. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
6. Tompson, J.R., E. Register, J. Curotto, M. Kurtz, and R. Kelly. 1998. A n i mproved protocol for the preparation of yeast cells for transformation by electroporation. Ye a st 14:565-571.
7. Sedman, T., P. Jõers, S. Kuusk, and J. Sedman. 2005. Helicase Hmi1 stimulates the synthesis of concatemeric mitochondrial DNA molecules in yeast Saccharomyces cerevisiae. Curr. Genet. 47:213-222.