Effect of Genomic Integration Location on Heterologous Protein Expression and Metabolic Engineering in E. coli

ACS Synthetic Biology

Volumn 6, Issue 4, 2017, Pages 710-720

  Englaender, Jacob A ^a   Jones, J Andrew ^b,f   Cress, Brady F ^b   Kuhlman, Thomas E ^c,d,e   Linhardt, Robert J ^a,b   Koffas, Mattheos A G ^a,b  

^a Research Rensselaer Polytechnic Institute   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^c University of Illinois at Urbana Champaign   (United States)

^d UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^e UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^f HAMILTON COLLEGE   (United States)

Author keywords

cinnamic acid; episomal expression; flavonoid production; genomic integration; metabolic burden; violacein

Indexed keywords

CINNAMIC ACID; PIGMENT; PLASMID VECTOR; PROTEIN; PROTEIN MCHERRY; SYNTHETIC DNA; UNCLASSIFIED DRUG; VIOLACEIN; AMMONIA LYASE; CINNAMIC ACID DERIVATIVE; ESCHERICHIA COLI PROTEIN; GIDB PROTEIN, E COLI; INDOLE DERIVATIVE; L-TYROSINE AMMONIA-LYASE; METHYLTRANSFERASE; PHOTOPROTEIN; RECA PROTEIN; RED FLUORESCENT PROTEIN;

ARTICLE; BACTERIAL CELL; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL METABOLISM; CONTROLLED STUDY; DNA INTEGRATION; DNA SEQUENCE; ESCHERICHIA COLI; GENE LOCUS; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LACTOSE OPERON; METABOLISM; PLASMID;

AMMONIA-LYASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMOSOMES, BACTERIAL; CINNAMATES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC LOCI; INDOLES; LAC OPERON; LUMINESCENT PROTEINS; METABOLIC ENGINEERING; METHYLTRANSFERASES; PLASMIDS; REC A RECOMBINASES;

EID: 85018473092     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00350     Document Type: Article

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