Corrigendum: Arsenite uncouples mitochondrial respiration and induces a warburg-like effect in caenorhabditis elegans [Toxicological Sciences, 152, 2, (2016), (349-362)], DOI:10.1093/toxsci/kfw093;Arsenite uncouples mitochondrial respiration and induces a warburg-like effect in Caenorhabditis elegans

Toxicological Sciences

Volumn 152, Issue 2, 2016, Pages 349-362

  Luz, Anthony L ^a   Godebo, Tewodros R ^a   Bhatt, Dhaval P ^b   Ilkayeva, Olga R ^b   Maurer, Laura L ^a   Hirschey, Matthew D ^b   Meyer, Joel N ^a  

^a DUKE UNIVERSITY   (United States)

^b DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Arsenite; Caenorhabditis elegans; Mitochondrial toxicity; Warburg effect

Indexed keywords

ADENOSINE TRIPHOSPHATE; ARSENIC TRIOXIDE; CELL NUCLEUS DNA; ELECTRON TRANSPORT CHAIN COMPLEX I; ELECTRON TRANSPORT CHAIN COMPLEX II; ELECTRON TRANSPORT CHAIN COMPLEX III; MULTIENZYME COMPLEX; PROTON; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; PYRUVIC ACID; UNCLASSIFIED DRUG; ARSENOUS ACID DERIVATIVE; PYRUVATE DEHYDROGENASE COMPLEX;

ARTICLE; AUTOPHAGY; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; EXPOSURE; GENE EXPRESSION; GENOTYPE ENVIRONMENT INTERACTION; GLYCOLYSIS; IN VIVO STUDY; METABOLIC INHIBITION; METABOLOMICS; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRIAL RESPIRATION; MITOCHONDRIAL TOXICITY; MOLECULAR BIOLOGY; NONHUMAN; PROTEIN EXPRESSION; RESPIRATORY CHAIN; WARBURG EFFECT; ANIMAL; COPY NUMBER VARIATION; DRUG EFFECTS; ELECTRON TRANSPORT; MASS SPECTROMETRY; METABOLISM; MITOCHONDRION; MUTATION; OXYGEN CONSUMPTION;

ADENOSINE TRIPHOSPHATE; ANIMALS; ARSENITES; CAENORHABDITIS ELEGANS; DNA COPY NUMBER VARIATIONS; ELECTRON TRANSPORT; GLYCOLYSIS; MASS SPECTROMETRY; MEMBRANE POTENTIAL, MITOCHONDRIAL; METABOLOMICS; MITOCHONDRIA; MUTATION; OXYGEN CONSUMPTION; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVIC ACID;

EID: 84992707503     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfw185     Document Type: Erratum

References (84)

1. An, J., Muoio, D. M., Shiota, M., Fujimoto, Y., Cline, G. W., Shulman, G. I., Koves, T. R., Stevens, R., Millington, D., and Newgard, C. B. (2004). Hepatic expression of malonyl-CoA decarboxylase reverses muscle, liver and whole-animal insulin resistance. Nat. Med. 10, 268-274.
2. Argos, M., Kalra, T., Rathouz, P. J., Chen, Y., Pierce, B., Parvez, F., Islam, T., Ahmed, A., Rakibuz-Zaman, M., and Hasan, R. (2010). Arsenic exposure from drinking water, and all-cause and chronic-disease mortalities in Bangladesh (HEALS): a prospective cohort study. Lancet 376, 252-258.
3. Arteel, G. E., Guo, L., Schlierf, T., Beier, J. I., Kaiser, J. P., Chen, T. S., Liu, M., Conklin, D. J., Miller, H. L., and von Montfort, C. (2008). Subhepatotoxic exposure to arsenic enhances lipopolysaccharide-induced liver injury in mice. Toxicol. Appl. Pharmacol. 226, 128-139.
4. Au, C., Benedetto, A., Anderson, J., Labrousse, A., Erikson, K., Ewbank, J. J., and Aschner, M. (2009). SMF-1, SMF-2 and SMF-3 DMT1 orthologues regulate and are regulated differentially by manganese levels in C. elegans. PloS One 4, e7792.
5. Belzacq, A. S., El Hamel, C., Vieira, H., Cohen, I., Haouzi, D., Metivier, D., Marchetti, P., Brenner, C., and Kroemer, G. (2001). Adenine nucleotide translocator mediates the mitochondrial membrane permeabilization induced by lonidamine, arsenite and CD437. Oncogene 20, 7579-7587.
6. Bergquist, E. R., Fischer, R. J., Sugden, K. D., and Martin, B. D. (2009). Inhibition by methylated organoarsenicals of the respiratory 2-oxo-acid dehydrogenases. J. Organomet. Chem. 694, 973-980.
7. Bolt, A. M., Douglas, R. M., and Klimecki, W. T. (2010). Arsenite exposure in human lymphoblastoid cell lines induces autophagy and coordinated induction of lysosomal genes. Toxicol. Lett. 199, 153-159.
8. Boyd, W. A., Smith, M. V., Kissling, G. E., and Freedman, J. H. (2010). Medium-and high-throughput screening of neurotoxicants using C. elegans. Neurotoxicol. Teratol. 32, 68-73.
9. Boyd, W. A., Smith, M. V., Kissling, G. E., Rice, J. R., Snyder, D. W., Portier, C. J., and Freedman, J. H. (2009). Application of a mathematical model to describe the effects of chlorpyrifos on Caenorhabditis elegans development. PLoS One 4, e7024.
10. Braeckman, B. P., Houthoofd, K., and Vanfleteren, J. R. 2009. Intermediary metabolism. WormBook. doi:10.1895/wormbook. 1.146.1 ISBN/ISSN: 1551-8507.
11. Brand, M. D., Affourtit, C., Esteves, T. C., Green, K., Lambert, A. J., Miwa, S., Pakay, J. L., and Parker, N. (2004). Mitochondrial superoxide: production, biological effects, and activation of uncoupling proteins. Free Radic. Biol. Med. 37, 755-767.
12. Brys, K., Castelein, N., Matthijssens, F., Vanfleteren, J. R., and Braeckman, B. P. (2010). Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans. BMC Biol. 8, 91.
13. Cline, D. J., Thorpe, C., and Schneider, J. P. (2003). Effects of As (III) binding on a-helical structure. J. Am. Chem. Soc. 125, 2923-2929.
14. Cooper, A. Jr., and Van Gundy, S. (1971). Ethanol production and utilization by Aphelenchus avenae and Caenorhabditis sp. J. Nematol. 3, 205.
15. DiMauro, S., and Schon, E. A. (2003). Mitochondrial respiratorychain diseases. N. Engl. J. Med. 348, 2656-2668.
16. Ditzel, E. J., Nguyen, T., Parker, P., and Camenisch, T. D. (2015). Effects of arsenite exposure during fetal development on energy metabolism and susceptibility to diet-induced fatty liver disease in male mice. Environ. Health Perspect. 124(2), 201-209.
17. Falk, M., Zhang, Z., Rosenjack, J., Nissim, I., Daikhin, E., Sedensky, M., Yudkoff, M., and Morgan, P. (2008). Metabolic pathway profiling of mitochondrial respiratory chain mutants in C. elegans. Mol. Genet. Metabol. 93, 388-397.
18. Föll, R. L., Pleyers, A., Lewandovski, G. J., Wermter, C., Hegemann, V., and Paul, R. J. (1999). Anaerobiosis in the nematode Caenorhabditis elegans. Compar. Biochem. Physiol. B: Biochem. Mol. Biol. 124, 269-280.
19. Gilbert-Diamond, D., Li, Z., Perry, A. E., Spencer, S. K., Gandolfi, A. J., and Karagas, M. R. (2013). A population-based case-control study of urinary arsenic species and squamous cell carcinoma in New Hampshire, USA. Environ. Health Perspect. 121, 1154.
20. HigashiI, T., Maruyama, E., Otani, T., and Sakamoto, Y. (1965). Studies on the isocitrate dehydrogenase. J. Biochem. 57, 793-798.
21. Hope, I., Arnold, J., McCarroll, D., Jun, G., Krupa, A., and Herbert, R. (1998). Promoter trapping identifies real genes in C. elegans. Mol. Gen. Genet. MGG 260, 300-308.
22. Hosseini, M. J., Shaki, F., Ghazi-Khansari, M., and Pourahmad, J. (2013). Toxicity of arsenic (III) on isolated liver mitochondria: a new mechanistic approach. Iranian J. Pharm. Res.: IJPR 12, 121.
23. Hughes, M. F. (2002). Arsenic toxicity and potential mechanisms of action. Toxicol. Lett. 133, 1-16.
24. Jensen, M. V., Joseph, J. W., Ilkayeva, O., Burgess, S., Lu, D., Ronnebaum, S. M., Odegaard, M., Becker, T. C., Sherry, A. D., and Newgard, C. B. (2006). Compensatory responses to pyruvate carboxylase suppression in islet b-cells preservation of glucose-stimulated insulin secretion. J. Biol. Chem. 281, 22342-22351.
25. Karagas, M. R., Stukel, T. A., and Tosteson, T. D. (2002). Assessment of cancer risk and environmental levels of arsenic in New Hampshire. Int. J. Hyg. Environ. Health 205, 85-94.
26. Karagas, M. R., Tosteson, T. D., Morris, J. S., Demidenko, E., Mott, L. A., Heaney, J., and Schned, A. (2004). Incidence of transitional cell carcinoma of the bladder and arsenic exposure in New Hampshire. Cancer Causes Control 15, 465-472.
27. Ke, Q., and Costa, M. (2006). Hypoxia-inducible factor-1 (HIF-1). Mol. Pharmacol. 70, 1469-1480.
28. Kim, J., Tchernyshyov, I., Semenza, G. L., and Dang, C. V. (2006). HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxia. Cell Metabol. 3, 177-185.
29. Kirkpatrick, D., Dale, K., Catania, J., and Gandolfi, A. (2003). Lowlevel arsenite causes accumulation of ubiquitinated proteins in rabbit renal cortical slices and HEK293 cells. Toxicol. Appl. Pharmacol. 186, 101-109.
30. Kitchin, K. T., and Wallace, K. (2008). The role of protein binding of trivalent arsenicals in arsenic carcinogenesis and toxicity. J. Inorg. Biochem. 102, 532-539.
31. Kondo, M., Senoo-Matsuda, N., Yanase, S., Ishii, T., Hartman, P. S., and Ishii, N. (2005). Effect of oxidative stress on translocation of DAF-16 in oxygen-sensitive mutants, mev-1 and gas-1 of Caenorhabditis elegans. Mechanisms Ageing Devel. 126, 637-641.
32. Lagido, C., McLaggan, D., and Glover, L. A. (2015). A screenable in vivo assay for mitochondrial modulators using transgenic bioluminescent Caenorhabditis elegans. JoVE (J. Vis. Exp.) e53083-e53083.
33. Lagido, C., Pettitt, J., Flett, A., and Glover, L. A. (2008). Bridging the phenotypic gap: real-time assessment of mitochondrial function and metabolism of the nematode Caenorhabditis elegans. BMC Physiology 8, 7.
34. Lapaille, M., Thiry, M., Perez, E., González-Halphen, D., Remacle, C., and Cardol, P. (2010). Loss of mitochondrial ATP synthase subunit beta (Atp2) alters mitochondrial and chloroplastic function and morphology in Chlamydomonas. Biochim. Biophys. Acta (BBA)-Bioenergetics 1797, 1533-1539.
35. Lesseur, C., Gilbert-Diamond, D., Andrew, A. S., Ekstrom, R. M., Li, Z., Kelsey, K. T., Marsit, C. J., and Karagas, M. R. (2012). A case-control study of polymorphisms in xenobiotic and arsenic metabolism genes and arsenic-related bladder cancer in New Hampshire. Toxicol. Lett. 210, 100-106.
36. Lewis, J. A., and Fleming, J. T. (1995). Basic culture methods. Methods Cell Biol. 48, 3-29.
37. Li, Y. N., Xi, M. M., Guo, Y., Hai, C. X., Yang, W. L., and Qin, X. J. (2014). NADPH oxidase-mitochondria axis-derived ROS mediate arsenite-induced HIF-1a stabilization by inhibiting prolyl hydroxylases activity. Toxicol. Lett. 224, 165-174.
38. Liao, V. H. C., and Yu, C. W. (2005). Caenorhabditis elegans gcs-1 confers resistance to arsenic-induced oxidative stress. Biometals 18, 519-528.
39. Liaw, J., Marshall, G., Yuan, Y., Ferreccio, C., Steinmaus, C., and Smith, A. H. (2008). Increased childhood liver cancer mortality and arsenic in drinking water in northern Chile. Cancer Epidemiol. Biomarkers Prevent. 17, 1982-1987.
40. Liu, X., Sadhukhan, S., Sun, S., Wagner, G. R., Hirschey, M. D., Qi, L., Lin, H., and Locasale, J. W. (2015). High resolution metabolomics with acyl-CoA profiling reveals widespread remodeling in response to diet. Mol. Cell. Proteomics MCP M114, 044859
41. Luz, A. L., Lagido, C., Hirschey, M. D., and Meyer, J. N. (2016). In vivo determination of mitochondrial function using luciferase-expressing Caenorhabditis elegans: contribution of oxidative phosphorylation, glycolysis, and fatty acid oxidation to toxicant-induced dysfunction. Curr. Protoc. Toxicol. 69,25.8.1-25.8.22.
42. Luz, A. L., Rooney, J. P., Kubik, L. L., Gonzalez, C. P., Song, D. H., and Meyer, J. N. (2015a). Mitochondrial Morphology and Fundamental Parameters of the Mitochondrial Respiratory Chain Are Altered in Caenorhabditis elegans Strains Deficient in Mitochondrial Dynamics and Homeostasis Processes. PloS One 10, e0130940.
43. Luz, A. L., Smith, L. L., Rooney, J. P., and Meyer, J. N. (2015b). Seahorse Xfe24 extracellular flux analyzer-based analysis of cellular respiration in Caenorhabditis elegans. Curr. Protoc. Toxicol. 66, 25.7.1-25.7.15.
44. Marshall, G., Ferreccio, C., Yuan, Y., Bates, M. N., Steinmaus, C., Selvin, S., Liaw, J., and Smith, A. H. (2007). Fifty-year study of lung and bladder cancer mortality in Chile related to arsenic in drinking water. J. Natl. Cancer Inst. 99, 920-928.
45. McBride, H. M., Neuspiel, M., and Wasiak, S. (2006). Mitochondria: more than just a powerhouse. Curr. Biol. 16, R551-R560.
46. Meyer, J. N., Lord, C. A., Yang, X. Y., Turner, E. A., Badireddy, A. R., Marinakos, S. M., Chilkoti, A., Wiesner, M. R., and Auffan, M. (2010). Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans. Aquat. Toxicol. 100, 140-150.
47. Morgan, P., Higdon, R., Kolker, N., Bauman, A., Ilkayeva, O., Newgard, C. B., Kolker, E., Steele, L., and Sedensky, M. (2015). Comparison of proteomic and metabolomic profiles of mutants of the mitochondrial respiratory chain in Caenorhabditis elegans. Mitochondrion 20, 95-102.
48. Myeku, N., and Figueiredo-Pereira, M. E. (2011). Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy association With sequestosome 1/p62. J. Biol. Chem. 286, 22426-22440.
49. Naranmandura, H., Xu, S., Sawata, T., Hao, W. H., Liu, H., Bu, N., Ogra, Y., Lou, Y. J., and Suzuki, N. (2011). Mitochondria are the main target organelle for trivalent monomethylarsonous acid (MMAIII)-induced cytotoxicity. Chem. Res. Toxicol. 24, 1094-1103.
50. Partridge, F. A., Tearle, A. W., Gravato-Nobre, M. J., Schafer, W. R., and Hodgkin, J. (2008). The C. elegans glycosyltransferase BUS-8 has two distinct and essential roles in epidermal morphogenesis. Devel. Biol. 317, 549-559.
51. Pfeiffer, M., Kayzer, E. B., Yang, X., Abramson, E., Kenaston, M. A., Lago, C. U., Lo, H. H., Sedensky, M. M., Lunceford, A., and Clarke, C. F. (2011). Caenorhabditis elegans UCP4 protein controls complex II-mediated oxidative phosphorylation through succinate transport. J. Biol. Chem. 286, 37712-37720.
52. Rahman, M. (2002). Arsenic and contamination of drinkingwater in Bangladesh: a public-health perspective. J. Health Popul. Nutr. 3, 193-197.
53. Rastogi, S., Borgo, B., Pazdernik, N., Fox, P., Mardis, E. R., Kohara, Y., Havranek, J., and Schedl, T. (2015). Caenorhabditis elegans glp-4 encodes a valyl aminoacyl tRNA synthetase. G3: Genesj Genomes Genet. 5, 2719-2728.
54. Ravenscroft, P., Brammer, H., and Richards, K. (2009). Arsenic Pollution: A Global Synthesis, vol. 28. West Sussex, United Kingdom: John Wiley & Sons.
55. Robey, R. B., Weisz, J., Kuemmerle, N., Salzberg, A. C., Berg, A., Brown, D. G., Kubik, L., Palorini, R., Al-Mulla, F., and Al-Temaimi, R. (2015). Metabolic reprogramming and dysregulated metabolism: cause, consequence and/or enabler of environmental carcinogenesis? Carcinogenesis 36, S203-S231.
56. Rooney, J., Luz, A., Gonzalez-Hunt, C., Bodhicharla, R., Ryde, I., Anbalagan, C., and Meyer, J. (2014). Effects of 50-fluoro-2-deoxyuridine on mitochondrial biology in Caenorhabditis elegans. Exp. Gerontol. 56, 69-76.
57. Rooney, J. P., Ryde, I. T., Sanders, L. H., Howlett, E. H., Colton, M. D., Germ, K. E., Mayer, G. D., Greenamyre, J. T., and Meyer, J. N. (2015). PCR based determination of mitochondrial DNA copy number in multiple species. In Palmeira, Carlos M., Rolo, Anabela P. eds, Mitochondrial Regulation, pp. 23-38. Clifton, NJ: Springer.
58. Sanchez-Soria, P., Broka, D., Quach, S., Hardwick, R. N., Cherrington, N. J., and Camenisch, T. D. (2014). Fetal exposure to arsenic results in hyperglycemia, hypercholesterolemia, and nonalcoholic fatty liver disease in adult mice. Journal of Toxicology and Health 1(1), 1.
59. Schaefer, A. M., Taylor, R. W., Turnbull, D. M., and Chinnery, P. F. (2004). The epidemiology of mitochondrial disorders-past, present and future. Biochim. Biophys. Acta (BBA)-Bioenergetics 1659, 115-120.
60. Shi, H., Shi, X., and Liu, K. J. (2004). Oxidative mechanism of arsenic toxicity and carcinogenesis. Mol. Cell. Biochem. 255, 67-78.
61. Shi, X., Wei, X., Koo, I., Schmidt, R. H., Yin, X., Kim, S. H., Vaughn, A., McClain, C. J., Arteel, G. E., and Zhang, X. (2013). Metabolomic analysis of the effects of chronic arsenic exposure in a mouse model of diet-induced fatty liver disease. J. Proteome Res. 13, 547-554.
62. Smith, A. H., Marshall, G., Yuan, Y., Ferreccio, C., Liaw, J., von Ehrenstein, O., Steinmaus, C., Bates, M. N., and Selvin, S. (2006). Increased mortality from lung cancer and bronchiectasis in young adults after exposure to arsenic in utero and in early childhood. Environ. Health Perspect. 1293-1296.
63. Stiernagle, T. (1999). Maintenance of C. elegans. C. Elegans 2, 51-67.
64. Tan, M., Schmidt, R. H., Beier, J. I., Watson, W. H., Zhong, H., States, J. C., and Arteel, G. E. (2011). Chronic subhepatotoxic exposure to arsenic enhances hepatic injury caused by high fat diet in mice. Toxicol. Appl. Pharmacol. 257, 356-364.
65. Thomas, D. J., Li, J., Waters, S. B., Xing, W., Adair, B. M., Drobna, Z., Devesa, V., and Styblo, M. (2007). Arsenic (+3 oxidation state) methyltransferase and the methylation of arsenicals. Exp. Biol. Med. 232, 3-13.
66. Tsang, W. Y., and Lemire, B. D. (2002). Mitochondrial genome content is regulated during nematode development. Biochem. Biophys. Res. Commun. 291, 8-16.
67. Tsang, W. Y., and Lemire, B. D. (2003). The role of mitochondria in the life of the nematode, Caenorhabditis elegans. Biochim. Biophys. Acta (BBA)-Mol. Basis Dis. 1638, 91-105.
68. Tsang, W. Y., Sayles, L. C., Grad, L. I., Pilgrim, D. B., and Lemire, B. D. (2001). Mitochondrial Respiratory chain deficiency in Caenorhabditis elegans results in developmental arrest and increased life span. J. Biol. Chem. 276, 32240-32246.
69. Tseng, Y. Y., Yu, C. W., and Liao, V. H. C. (2007). Caenorhabditis elegans expresses a functional ArsA. FEBS J. 274, 2566-2572.
70. Wang, T. S., Hsu, T. Y., Chung, C. H., Wang, A. S., Bau, D. T., and Jan, K. Y. (2001). Arsenite induces oxidative DNA adducts and DNA-protein cross-links in mammalian cells. Free Radic. Biol. Med. 31, 321-330.
71. Wang, Y., Singh, U., and Mueller, D. M. (2007). Mitochondrial genome integrity mutations uncouple the yeast Saccharomyces cerevisiae ATP synthase. J. Biol. Chem. 282, 8228-8236.
72. Watanabe, M., Mitani, N., Ishii, N., and Miki, K. (2005). A mutation in a cuticle collagen causes hypersensitivity to the endocrine disrupting chemical, bisphenol A, in Caenorhabditis elegans. Mutat. Res. Fund. Mol. Mechanisms Mut. 570, 71-80.
73. Weber, C. I. (1991). Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms. Cincinnati, Ohio: Environmental Monitoring Systems Laboratory, Office of Research and Development, US Environmental Protection Agency.
74. Wiȩ, M. R., and Wojtczak, L. (1997). Involvement of the dicarboxylate carrier in the protonophoric action of long-chain fatty acids in mitochondria. Biochem. Biophys. Res. Commun. 232, 414-417.
75. Wu, J. Y., Kao, H. J., Li, S. C., Stevens, R., Hillman, S., Millington, D., and Chen, Y. T. (2004). ENU mutagenesis identifies mice with mitochondrial branched-chain aminotransferase deficiency resembling human maple syrup urine disease. J. Clin. Investig. 113, 434.
76. Yang, W., and Hekimi, S. (2010). Two modes of mitochondrial dysfunction lead independently to lifespan extension in Caenorhabditis elegans. Aging Cell 9, 433-447.
77. Yochem, J., and Greenwald, I. (1989). glp-1 and lin-12, genes implicated in distinct cell-cell interactions in C. elegans, encode similar transmembrane proteins. Cell 58, 553-563.
78. Yu, C. W., How, C. M., and Liao, V. H. C. (2016). Arsenite exposure accelerates aging process regulated by the transcription factor DAF-16/FOXO in Caenorhabditis elegans. Chemosphere. 150, 632-638.
79. Yu, H. S., Liao, W. T., and Chai, C. Y. (2006). Arsenic carcinogenesis in the skin. J. Biomed. Sci. 13, 657-666.
80. Yuan, Y., Marshall, G., Ferreccio, C., Steinmaus, C., Liaw, J., Bates, M., and Smith, A. H. (2010). Kidney cancer mortality: fiftyyear latency patterns related to arsenic exposure. Epidemiology 21, 103-108.
81. Zhang, T., Qi, Y., Liao, M., Xu, M., Bower, K., Frank, J., Shen, H. M., Luo, J., Shi, X., and Chen, G. (2012). Autophagy is a cell selfprotective mechanism against arsenic-induced cell transfomation. Toxicol. Sci. 130(2):298-308.
82. Zhao, C. Q., Young,M. R., Diwan, B. A., Coogan, T. P., andWaalkes, M. P. (1997). Association of arsenic-induced malignant transformation with DNA hypomethylation and aberrant gene expression. Proc. Natl. Acad. Sci. USA 94, 10907-10912.
83. Zhao, F., Malm, S. W., Hinchman, A. N., Li, H., Beeks, C. G., and Klimecki, W. T. (2014). Arsenite-induced pseudo-hypoxia results in loss of anchorage-dependent growth in BEAS-2B pulmonary epithelial cells. PloS One 9, e114549.
84. Zhao, F., Severson, P., Pacheco, S., Futscher, B. W., and Klimecki, W. T. (2013). Arsenic exposure induces the Warburg effect in cultured human cells. Toxicol. Appl. Pharmacol. 271, 72-77.