Bridging the phenotypic gap: Real-time assessment of mitochondrial function and metabolism of the nematode Caenorhabditis elegans

BMC Physiology

Volumn 8, Issue 1, 2008, Pages

  Lagido, Cristina ^a   Pettitt, Jonathan ^a   Flett, Aileen ^a   Glover, L Anne ^a  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; AZIDE; FIREFLY LUCIFERASE; PHOTOPROTEIN;

ARTICLE; BIOLUMINESCENCE; CAENORHABDITIS ELEGANS; CELL LEVEL; ELECTRON TRANSPORT; GENE EXPRESSION; GENETIC CODE; IN VITRO STUDY; IN VIVO STUDY; METABOLISM; MITOCHONDRION; NEMATODE; NONHUMAN; PHENOTYPE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; RNA INTERFERENCE; WORM; ANIMAL; COMPUTER SYSTEM; EVALUATION; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; GENETICS; METHODOLOGY; PHYSIOLOGY; TRANSGENIC ANIMAL; WHOLE BODY IMAGING;

ADENOSINE TRIPHOSPHATE; ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; COMPUTER SYSTEMS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; MOLECULAR PROBE TECHNIQUES; PHENOTYPE; WHOLE BODY IMAGING;

EID: 43149110004     PISSN: None     EISSN: 14726793     Source Type: Journal    
DOI: 10.1186/1472-6793-8-7     Document Type: Article

References (42)

1. Energy hypometabolism in posterior cingulate cortex of Alzheimer's patients: superficial laminar cytochrome oxidase associated with disease duration. J Valla JD Berndt F Gonzalez-Lima, J Neurosci 2001 21 13 4923 4930 11425920
2. Mitochondrial dysfunction in the elderly: possible role in insulin resistance. KF Petersen D Befroy S Dufour J Dziura C Ariyan DL Rothman L DiPietro GW Cline GI Shulman, Science 2003 300 5622 1140 1142 10.1126/science.1082889 12750520
3. Mitochondrial targets of drug toxicity. KB Wallace AA Starkov, Annu Rev Pharmacol Toxicol 2000 40 353 388 10.1146/annurev.pharmtox.40.1.353 10836141
4. Long-lived C. elegans mitochondrial mutants as a model for human mitochondrial-associated diseases. N Ventura SL Rea R Testi, Exp Gerontol 2006 41 10 974 991 10.1016/j.exger.2006.06.060 16945497
5. Mitochondrial respiration and ATP production are significantly impaired in striatal cells expressing mutant huntingtin. T Milakovic GV Johnson, J Biol Chem 2005 280 35 30773 30782 10.1074/jbc.M504749200 15983033
6. Chronic systemic pesticide exposure reproduces features of Parkinson's disease. R Betarbet TB Sherer G MacKenzie M Garcia-Osuna AV Panov JT Greenamyre, Nat Neurosci 2000 3 12 1301 1306 10.1038/81834 11100151
7. Rates of behavior and aging specified by mitochondrial function during development. A Dillin AL Hsu N Arantes-Oliveira J Lehrer-Graiwer H Hsin AG Fraser RS Kamath J Ahringer C Kenyon, Science 2002 298 5602 2398 2401 10.1126/science.1077780 12471266
8. DAF-2 pathway mutations and food restriction in aging Caenorhabditis elegans differentially affect metabolism. K Houthoofd BP Braeckman I Lenaerts K Brys F Matthijssens A De Vreese S Van Eygen JR Vanfleteren, Neurobiol Aging 2005 26 5 689 696 10.1016/j.neurobiolaging.2004.06.011 15708444
9. Metabolism, physiology and stress defense in three aging Ins/IGF-1 mutants of the nematode Caenorhabditis elegans. K Houthoofd MA Fidalgo D Hoogewijs BP Braeckman I Lenaerts K Brys F Matthijssens A De Vreese S Van Eygen MJ Munoz JR Vanfleteren, Aging Cell 2005 4 2 87 95 10.1111/j.1474-9726.2005. 00150.x 15771612
10. In vivo phosphorus-31 nuclear magnetic resonance reveals lowered ATP during heat shock of Tetrahymena. RC Findley RJ Gillies RG Schulman, Science 1983 219 4589 1223 1225 10.1126/science.6828852 6828852
11. The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans. J Apfeld G O'Connor T McDonagh PS DiStefano R Curtis, Genes Dev 2004 18 24 3004 3009 15574588 10.1101/gad.1255404
12. Changes of adenine nucleotide levels in Chara internodes during metabolic inhibition. Y Takeuchi U Kishimoto, Plant and Cell Physiology 1983 24 8 1401 1409 http://pcp.oxfordjournals.org/cgi/content/abstract/24/8/1401
13. Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death. BS Tiwari B Belenghi A Levine, Plant Physiol 2002 128 4 1271 1281 11950976 10.1104/pp.010999
14. Effect of anoxia on intracellular ATP, Na+i, Ca2+i, Mg2+i, and cytotoxicity in rat hepatocytes. A Gasbarrini AB Borle H Farghali C Bender A Francavilla D Van Thiel, J Biol Chem 1992 267 10 6654 6663 1637381
15. No reduction of metabolic rate in food restricted Caenorhabditis elegans. K Houthoofd BP Braeckman I Lenaerts K Brys A De Vreese S Van Eygen JR Vanfleteren, Exp Gerontol 2002 37 12 1359 1369 10.1016/S0531-5565(02)00172-9 12559405
16. Contributions of ATP, GTP, and redox state to nutritional stress activation of the Bacillus subtilis sigmaB transcription factor. S Zhang WG Haldenwang, J Bacteriol 2005 187 22 7554 7560 16267279 10.1128/JB.187.22.7554- 7560.2005
17. Role of the AMP-activated protein kinase in the cellular stress response. JM Corton JG Gillespie DG Hardie, Curr Biol 1994 4 4 315 324 10.1016/S0960-9822(00)00070-1 7922340
18. Firefly luciferase gene: structure and expression in mammalian cells. JR de Wet KV Wood M DeLuca DR Helinski S Subramani, Mol Cell Biol 1987 7 2 725 737 3821727
19. Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors. C Lagido J Pettitt AJ Porter GI Paton LA Glover, FEBS Lett 2001 493 1 36 39 10.1016/S0014-5793(01)02271-2 11278001
20. Continuous monitoring of ATP levels in living insulin secreting cells expressing cytosolic firefly luciferase. P Maechler H Wang CB Wollheim, FEBS Lett 1998 422 3 328 332 10.1016/S0014-5793(97)01618-9 9498809
21. Relationship between growth rate and ATP concentration in Escherichia coli: a bioassay for available cellular ATP. DA Schneider RL Gourse, J Biol Chem 2004 279 9 8262 8268 10.1074/jbc.M311996200 14670952
22. A role for Caenorhabditis elegans in understanding the function and interactions of human disease genes. E Culetto DB Sattelle, Hum Mol Genet 2000 9 6 869 877 10.1093/hmg/9.6.869 10767309
23. No reduction of energy metabolism in Clk mutants. BP Braeckman K Houthoofd K Brys I Lenaerts A De Vreese S Van Eygen H Raes JR Vanfleteren, Mech Ageing Dev 2002 123 11 1447 1456 10.1016/S0047-6374(02)00085-4 12425951
24. Cations and anions: inhibitors and interactions in metabolism and in enzyme activity. EJ Hewitt DJD Nicholas, Metabolic inhibitors London, Academic Press, Hochster RM, Quastel JH, 1963 311 436
25. Exposure to the metabolic inhibitor sodium azide induces stress protein expression and thermotolerance in the nematode Caenorhabditis elegans. MR Massie EM Lapoczka KD Boggs KE Stine GE White, Cell Stress Chaperones 2003 8 1 1 7 12820649 10.1379/1466-1268(2003)8<1:ETTMIS>2.0.CO;2
26. Feeding is inhibited by sublethal concentrations of toxicants and by heat stress in the nematode Caenorhabditis elegans: relationship to the cellular stress response. D Jones EP Candido, J Exp Zool 1999 284 2 147 157 10.1002/(SICI)1097-010X(19990701)284:2<147::AID-JEZ4>3.0.CO;2-Z 10404644
27. Modulation of firefly luciferase stability and impact on studies of gene regulation. JF Thompson LS Hayes DB Lloyd, Gene 1991 103 2 171 177 10.1016/0378-1119(91)90270-L 1889744
28. Monitoring immediate-early gene expression through firefly luciferase imaging of HRS/J hairless mice. AM Collaco ME Geusz, BMC Physiol 2003 3 8 12927048 10.1186/1472-6793-3-8
29. Novel features of Drosophila period transcription revealed by real-time luciferase reporting. C Brandes JD Plautz R Stanewsky CF Jamison M Straume KV Wood SA Kay JC Hall, Neuron 1996 16 4 687 692 10.1016/S0896-6273(00)80088-4 8607986
30. Monitoring of spatial expression of firefly luciferase in transformed zebrafish. R Mayerhofer K Araki AA Szalay, J Biolumin Chemilumin 1995 10 5 271 275 10.1002/bio.1170100503 8533608
31. A novel circadian phenotype based on firefly luciferase expression in transgenic plants. AJ Millar SR Short NH Chua SA Kay, Plant Cell 1992 4 9 1075 1087 1392609 10.1105/tpc.4.9.1075
32. Firefly luciferase is targeted to peroxisomes in mammalian cells. GA Keller S Gould M Deluca S Subramani, Proc Natl Acad Sci U S A 1987 84 10 3264 3268 3554235 10.1073/pnas.84.10.3264
33. Protein denaturation during heat shock and related stress. Escherichia coli beta-galactosidase and Photinus pyralis luciferase inactivation in mouse cells. VT Nguyen M Morange O Bensaude, J Biol Chem 1989 264 18 10487 10492 2499580
34. The modified firefly luciferase reporter gene (luc+) but not Renilla luciferase is induced by all-trans retinoic acid in MCF-7 breast cancer cells. T Kogai Y Kanamoto GA Brent, Breast Cancer Res Treat 2003 78 1 119 126 10.1023/A:1022179717847 12611464
35. Attenuation of green fluorescent protein half-life in mammalian cells. P Corish C Tyler-Smith, Protein Eng 1999 12 12 1035 1040 10.1093/protein/12.12. 1035 10611396
36. Caenorhabditis elegans SUR-5, a novel but conserved protein, negatively regulates LET-60 Ras activity during vulval induction. T Gu S Orita M Han, Mol Cell Biol 1998 18 8 4556 4564 9671465
37. DNA transformation. C Mello A Fire, Methods in Cell Biology San Diego, Academic Press, Epstein HF, Shakes DC, Caenorhabditis elegans: Modern Biological Analysis of an organism. 1995 48 451 482 8531738
38. Mutagenesis. P Anderson, Methods in Cell Biology San Diego, Academic Press 1995 48 31 58 8531732
39. Basic culture methods. JA Lewis JT Fleming, Methods in Cell Biology San Diego, Academic Press, Epstein HF, Shakes DC, Caenorhabditis elegans: Modern Biological Analysis of an organism. 1995 48 3 29 8531730
40. pha-2 encodes the C. elegans ortholog of the homeodomain protein HEX and is required for the formation of the pharyngeal isthmus. C Mörck M Rauthan F Wagberg M Pilon, Dev Biol 2004 272 2 403 418 10.1016/j.ydbio.2004.05.011 15282157
41. Reverse genetics. J Ahringer (ed.), WormBook The C. elegans Research Community, WormBook, http://www.wormbook.org/chapters/www_introreversegenetics/ introreversegenetics.pdf 10.1895/wormbook.1.47.1
42. Biometry: the principles and practice of statistics in biological research. RR Sokal FJ Rohlf, New York, W. H. Freeman and Company 3rd 1995 887