메뉴 건너뛰기




Volumn 88, Issue 6, 2016, Pages 1071-1081

Genome editing of model oleaginous microalgae Nannochloropsis spp. by CRISPR/Cas9

Author keywords

clustered regularly interspaced short palindromic repeat Cas system; genome editing; Nannochloropsis spp.; nitrate reductase; oleaginous microalgae; technical advance

Indexed keywords

CHLORINE COMPOUNDS; ENZYMES; GENE ENCODING; MICROORGANISMS; NITRATES; POLYMERASE CHAIN REACTION; SODIUM NITRATE;

EID: 84991737543     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/tpj.13307     Document Type: Article
Times cited : (225)

References (62)
  • 1
    • 6044252163 scopus 로고    scopus 로고
    • The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism
    • Armbrust, E.V., Berges, J.A., Bowler, C. et al. (2004) The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science, 306, 79–86.
    • (2004) Science , vol.306 , pp. 79-86
    • Armbrust, E.V.1    Berges, J.A.2    Bowler, C.3
  • 2
    • 78049477633 scopus 로고    scopus 로고
    • The Chlorella variabilis NC64A genome reveals adaptation to photosymbiosis, coevolution with viruses, and cryptic sex
    • Blanc, G., Duncan, G., Agarkova, I. et al. (2010) The Chlorella variabilis NC64A genome reveals adaptation to photosymbiosis, coevolution with viruses, and cryptic sex. Plant Cell, 22, 2943–2955.
    • (2010) Plant Cell , vol.22 , pp. 2943-2955
    • Blanc, G.1    Duncan, G.2    Agarkova, I.3
  • 3
    • 56249098614 scopus 로고    scopus 로고
    • The Phaeodactylum genome reveals the evolutionary history of diatom genomes
    • Bowler, C., Allen, A.E., Badger, J.H. et al. (2008) The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature, 456, 239–244.
    • (2008) Nature , vol.456 , pp. 239-244
    • Bowler, C.1    Allen, A.E.2    Badger, J.H.3
  • 4
    • 77953001382 scopus 로고    scopus 로고
    • The Ectocarpus genome and the independent evolution of multicellularity in brown algae
    • Cock, J.M., Sterck, L., Rouze, P. et al. (2010) The Ectocarpus genome and the independent evolution of multicellularity in brown algae. Nature, 465, 617–621.
    • (2010) Nature , vol.465 , pp. 617-621
    • Cock, J.M.1    Sterck, L.2    Rouze, P.3
  • 6
    • 84901813823 scopus 로고    scopus 로고
    • Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
    • Daboussi, F., Leduc, S., Marechal, A. et al. (2014) Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology. Nat. Commun. 5, 3831.
    • (2014) Nat. Commun. , vol.5 , pp. 3831
    • Daboussi, F.1    Leduc, S.2    Marechal, A.3
  • 7
    • 33746830079 scopus 로고    scopus 로고
    • Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features
    • Derelle, E., Ferraz, C., Rombauts, S. et al. (2006) Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features. Proc. Natl. Acad. Sci. U S A, 103, 11647–11652.
    • (2006) Proc. Natl. Acad. Sci. U S A , vol.103 , pp. 11647-11652
    • Derelle, E.1    Ferraz, C.2    Rombauts, S.3
  • 9
    • 84957605863 scopus 로고    scopus 로고
    • Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
    • Doench, J.G., Fusi, N., Sullender, M. et al. (2016) Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat. Biotechnol. 34, 184–191.
    • (2016) Nat. Biotechnol. , vol.34 , pp. 184-191
    • Doench, J.G.1    Fusi, N.2    Sullender, M.3
  • 11
    • 84949571274 scopus 로고    scopus 로고
    • Genomic foundation of starch-to-lipid switch in oleaginous Chlorella spp
    • Fan, J., Ning, K., Zeng, X. et al. (2015) Genomic foundation of starch-to-lipid switch in oleaginous Chlorella spp. Plant Physiol. 169, 2444–2461.
    • (2015) Plant Physiol. , vol.169 , pp. 2444-2461
    • Fan, J.1    Ning, K.2    Zeng, X.3
  • 12
    • 84978438340 scopus 로고    scopus 로고
    • DNA-guided genome editing using the Natronobacterium gregoryi Argonaute
    • Gao, F., Shen, X.Z., Jiang, F., Wu, Y. and Han, C. (2016) DNA-guided genome editing using the Natronobacterium gregoryi Argonaute. Nat. Biotechnol. 34, 768–773.
    • (2016) Nat. Biotechnol. , vol.34 , pp. 768-773
    • Gao, F.1    Shen, X.Z.2    Jiang, F.3    Wu, Y.4    Han, C.5
  • 13
    • 84865142852 scopus 로고    scopus 로고
    • Exploiting diversity and synthetic biology for the production of algal biofuels
    • Georgianna, D.R. and Mayfield, S.P. (2012) Exploiting diversity and synthetic biology for the production of algal biofuels. Nature, 488, 329–335.
    • (2012) Nature , vol.488 , pp. 329-335
    • Georgianna, D.R.1    Mayfield, S.P.2
  • 14
    • 84880571335 scopus 로고    scopus 로고
    • CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes
    • Gilbert, L.A., Larson, M.H., Morsut, L. et al. (2013) CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell, 154, 442–451.
    • (2013) Cell , vol.154 , pp. 442-451
    • Gilbert, L.A.1    Larson, M.H.2    Morsut, L.3
  • 15
    • 79952725430 scopus 로고    scopus 로고
    • Niche of harmful alga Aureococcus anophagefferens revealed through ecogenomics
    • Gobler, C.J., Berry, D.L., Dyhrman, S.T. et al. (2011) Niche of harmful alga Aureococcus anophagefferens revealed through ecogenomics. Proc. Natl. Acad. Sci. U S A, 108, 4352–4357.
    • (2011) Proc. Natl. Acad. Sci. U S A , vol.108 , pp. 4352-4357
    • Gobler, C.J.1    Berry, D.L.2    Dyhrman, S.T.3
  • 16
    • 43549102657 scopus 로고    scopus 로고
    • Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
    • Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M. and Darzins, A. (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 54, 621–639.
    • (2008) Plant J. , vol.54 , pp. 621-639
    • Hu, Q.1    Sommerfeld, M.2    Jarvis, E.3    Ghirardi, M.4    Posewitz, M.5    Seibert, M.6    Darzins, A.7
  • 17
    • 84903300632 scopus 로고    scopus 로고
    • Genome-wide identification of transcription factors and transcription-factor binding sites in oleaginous microalgae Nannochloropsis
    • Hu, J., Wang, D., Li, J., Jing, G., Ning, K. and Xu, J. (2014) Genome-wide identification of transcription factors and transcription-factor binding sites in oleaginous microalgae Nannochloropsis. Sci. Rep. 4, 5454.
    • (2014) Sci. Rep. , vol.4 , pp. 5454
    • Hu, J.1    Wang, D.2    Li, J.3    Jing, G.4    Ning, K.5    Xu, J.6
  • 18
    • 84923204103 scopus 로고    scopus 로고
    • Raman spectroscopy provides a rapid, non-invasive method for quantitation of starch in live, unicellular microalgae
    • Ji, Y., He, Y., Cui, Y., Wang, T., Wang, Y., Li, Y., Huang, W.E. and Xu, J. (2014) Raman spectroscopy provides a rapid, non-invasive method for quantitation of starch in live, unicellular microalgae. Biotechnol. J. 9, 1512–1518.
    • (2014) Biotechnol. J. , vol.9 , pp. 1512-1518
    • Ji, Y.1    He, Y.2    Cui, Y.3    Wang, T.4    Wang, Y.5    Li, Y.6    Huang, W.E.7    Xu, J.8
  • 19
    • 84921324786 scopus 로고    scopus 로고
    • Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions
    • Jia, J., Han, D., Gerken, H.G., Li, Y., Sommerfeld, M., Hu, Q. and Xu, J. (2015) Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions. Algal Res. 7, 66–77.
    • (2015) Algal Res. , vol.7 , pp. 66-77
    • Jia, J.1    Han, D.2    Gerken, H.G.3    Li, Y.4    Sommerfeld, M.5    Hu, Q.6    Xu, J.7
  • 20
    • 84908334356 scopus 로고    scopus 로고
    • Successful transient expression of Cas9 and single guide RNA genes in Chlamydomonas reinhardtii
    • Jiang, W., Bruggeman, A.J., Horken, K.M., Plucinak, T.M. and Weeks, D.P. (2014) Successful transient expression of Cas9 and single guide RNA genes in Chlamydomonas reinhardtii. Eukaryot. Cell, 13, 1465–1469.
    • (2014) Eukaryot. Cell , vol.13 , pp. 1465-1469
    • Jiang, W.1    Bruggeman, A.J.2    Horken, K.M.3    Plucinak, T.M.4    Weeks, D.P.5
  • 22
    • 84943662980 scopus 로고    scopus 로고
    • Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: overexpression of endogenous Δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG
    • Kaye, Y., Grundman, O., Leu, S., Zarka, A., Zorin, B., Didi-Cohen, S., Khozin-Goldberg, I. and Boussiba, S. (2015) Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: overexpression of endogenous Δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG. Algal Res. 11, 387–398.
    • (2015) Algal Res. , vol.11 , pp. 387-398
    • Kaye, Y.1    Grundman, O.2    Leu, S.3    Zarka, A.4    Zorin, B.5    Didi-Cohen, S.6    Khozin-Goldberg, I.7    Boussiba, S.8
  • 23
    • 84855505372 scopus 로고    scopus 로고
    • High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp
    • Kilian, O., Benemann, C.S., Niyogi, K.K. and Vick, B. (2011) High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proc. Natl. Acad. Sci. U S A, 108, 21265–21269.
    • (2011) Proc. Natl. Acad. Sci. U S A , vol.108 , pp. 21265-21269
    • Kilian, O.1    Benemann, C.S.2    Niyogi, K.K.3    Vick, B.4
  • 26
    • 84883785822 scopus 로고    scopus 로고
    • Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9
    • Li, J.F., Norville, J.E., Aach, J., McCormack, M., Zhang, D., Bush, J., Church, G.M. and Sheen, J. (2013) Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat. Biotechnol. 31, 688–691.
    • (2013) Nat. Biotechnol. , vol.31 , pp. 688-691
    • Li, J.F.1    Norville, J.E.2    Aach, J.3    McCormack, M.4    Zhang, D.5    Bush, J.6    Church, G.M.7    Sheen, J.8
  • 27
    • 84912113111 scopus 로고    scopus 로고
    • High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product
    • Li, F., Gao, D. and Hu, H. (2014a) High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product. Biosci. Biotechnol. Biochem. 78, 812–817.
    • (2014) Biosci. Biotechnol. Biochem. , vol.78 , pp. 812-817
    • Li, F.1    Gao, D.2    Hu, H.3
  • 28
    • 84901321937 scopus 로고    scopus 로고
    • Choreography of transcriptomes and lipidomes of Nannochloropsis reveals the mechanisms of oil synthesis in microalgae
    • Li, J., Han, D., Wang, D. et al. (2014b) Choreography of transcriptomes and lipidomes of Nannochloropsis reveals the mechanisms of oil synthesis in microalgae. Plant Cell, 26, 1645–1665.
    • (2014) Plant Cell , vol.26 , pp. 1645-1665
    • Li, J.1    Han, D.2    Wang, D.3
  • 29
    • 84902345325 scopus 로고    scopus 로고
    • Efficient gene targeting and removal of foreign DNA by homologous recombination in the picoeukaryote Ostreococcus
    • Lozano, J.C., Schatt, P., Botebol, H., Verge, V., Lesuisse, E., Blain, S., Carre, I.A. and Bouget, F.Y. (2014) Efficient gene targeting and removal of foreign DNA by homologous recombination in the picoeukaryote Ostreococcus. Plant J., 78, 1073–1083.
    • (2014) Plant J. , vol.78 , pp. 1073-1083
    • Lozano, J.C.1    Schatt, P.2    Botebol, H.3    Verge, V.4    Lesuisse, E.5    Blain, S.6    Carre, I.A.7    Bouget, F.Y.8
  • 30
    • 84928827033 scopus 로고    scopus 로고
    • Phytohormones in microalgae: a new opportunity for microalgal biotechnology?
    • Lu, Y. and Xu, J. (2015) Phytohormones in microalgae: a new opportunity for microalgal biotechnology? Trends Plant Sci. 20, 273–282.
    • (2015) Trends Plant Sci. , vol.20 , pp. 273-282
    • Lu, Y.1    Xu, J.2
  • 31
    • 84924808376 scopus 로고    scopus 로고
    • Antagonistic roles of abscisic acid and cytokinin during response to nitrogen depletion in oleaginous microalga Nannochloropsis oceanica expand the evolutionary breadth of phytohormone function
    • Lu, Y., Tarkowska, D., Tureckova, V., Luo, T., Xin, Y., Li, J., Wang, Q., Jiao, N., Strnad, M. and Xu, J. (2014a) Antagonistic roles of abscisic acid and cytokinin during response to nitrogen depletion in oleaginous microalga Nannochloropsis oceanica expand the evolutionary breadth of phytohormone function. Plant J. 80, 52–68.
    • (2014) Plant J. , vol.80 , pp. 52-68
    • Lu, Y.1    Tarkowska, D.2    Tureckova, V.3    Luo, T.4    Xin, Y.5    Li, J.6    Wang, Q.7    Jiao, N.8    Strnad, M.9    Xu, J.10
  • 32
    • 84902539648 scopus 로고    scopus 로고
    • Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica
    • Lu, Y., Zhou, W., Wei, L., Li, J., Jia, J., Li, F., Smith, S.M. and Xu, J. (2014b) Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica. Biotechnol. Biofuels, 7, 81.
    • (2014) Biotechnol. Biofuels , vol.7 , pp. 81
    • Lu, Y.1    Zhou, W.2    Wei, L.3    Li, J.4    Jia, J.5    Li, F.6    Smith, S.M.7    Xu, J.8
  • 33
    • 11144354393 scopus 로고    scopus 로고
    • Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D
    • Matsuzaki, M., Misumi, O., Shin, I.T. et al. (2004) Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature, 428, 653–657.
    • (2004) Nature , vol.428 , pp. 653-657
    • Matsuzaki, M.1    Misumi, O.2    Shin, I.T.3
  • 34
    • 35348896591 scopus 로고    scopus 로고
    • The Chlamydomonas genome reveals the evolution of key animal and plant functions
    • Merchant, S.S., Prochnik, S.E., Vallon, O. et al. (2007) The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science, 318, 245–250.
    • (2007) Science , vol.318 , pp. 245-250
    • Merchant, S.S.1    Prochnik, S.E.2    Vallon, O.3
  • 35
    • 84964659819 scopus 로고    scopus 로고
    • A CRISPR/Cas9 system adapted for gene editing in marine algae
    • Nymark, M., Sharma, A.K., Sparstad, T., Bones, A.M. and Winge, P. (2016) A CRISPR/Cas9 system adapted for gene editing in marine algae. Sci. Rep. 6, 24951.
    • (2016) Sci. Rep. , vol.6 , pp. 24951
    • Nymark, M.1    Sharma, A.K.2    Sparstad, T.3    Bones, A.M.4    Winge, P.5
  • 36
    • 83055194317 scopus 로고    scopus 로고
    • Nuclear monoploidy and asexual propagation of Nannochloropsis oceanica (eustigmatophyceae) as revealed by its genome sequence
    • Pan, K., Qin, J., Li, S., Dai, W., Zhu, B., Yuanchun, J., Wengong, Y., Guanpin, Y. and Dongfang, L. (2011) Nuclear monoploidy and asexual propagation of Nannochloropsis oceanica (eustigmatophyceae) as revealed by its genome sequence. J. Phycol. 47, 1425–1432.
    • (2011) J. Phycol. , vol.47 , pp. 1425-1432
    • Pan, K.1    Qin, J.2    Li, S.3    Dai, W.4    Zhu, B.5    Yuanchun, J.6    Wengong, Y.7    Guanpin, Y.8    Dongfang, L.9
  • 37
    • 84942237602 scopus 로고    scopus 로고
    • Generation of random mutants to improve light-use efficiency of Nannochloropsis gaditana cultures for biofuel production
    • Perin, G., Bellan, A., Segalla, A., Meneghesso, A., Alboresi, A. and Morosinotto, T. (2015) Generation of random mutants to improve light-use efficiency of Nannochloropsis gaditana cultures for biofuel production. Biotechnol. Biofuels, 8, 161.
    • (2015) Biotechnol. Biofuels , vol.8 , pp. 161
    • Perin, G.1    Bellan, A.2    Segalla, A.3    Meneghesso, A.4    Alboresi, A.5    Morosinotto, T.6
  • 38
    • 84917682436 scopus 로고    scopus 로고
    • Climate warming and agricultural stressors interact to determine stream periphyton community composition
    • Piggott, J.J., Salis, R.K., Lear, G., Townsend, C.R. and Matthaei, C.D. (2015) Climate warming and agricultural stressors interact to determine stream periphyton community composition. Glob. Chang. Biol. 21, 206–222.
    • (2015) Glob. Chang. Biol. , vol.21 , pp. 206-222
    • Piggott, J.J.1    Salis, R.K.2    Lear, G.3    Townsend, C.R.4    Matthaei, C.D.5
  • 40
    • 77954606278 scopus 로고    scopus 로고
    • Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri
    • Prochnik, S.E., Umen, J., Nedelcu, A.M. et al. (2010) Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri. Science, 329, 223–226.
    • (2010) Science , vol.329 , pp. 223-226
    • Prochnik, S.E.1    Umen, J.2    Nedelcu, A.M.3
  • 42
    • 84865281597 scopus 로고    scopus 로고
    • Robust expression and secretion of xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide
    • Rasala, B.A., Lee, P.A., Shen, Z., Briggs, S.P., Mendez, M. and Mayfield, S.P. (2012) Robust expression and secretion of xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PLoS ONE, 7, e43349.
    • (2012) PLoS ONE , vol.7
    • Rasala, B.A.1    Lee, P.A.2    Shen, Z.3    Briggs, S.P.4    Mendez, M.5    Mayfield, S.P.6
  • 43
    • 84919762182 scopus 로고    scopus 로고
    • Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila
    • Ren, X., Yang, Z., Xu, J. et al. (2014) Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila. Cell Rep. 9, 1151–1162.
    • (2014) Cell Rep. , vol.9 , pp. 1151-1162
    • Ren, X.1    Yang, Z.2    Xu, J.3
  • 44
    • 84961574790 scopus 로고    scopus 로고
    • CRISPR/Cas9: an advanced tool for editing plant genomes
    • Samanta, M.K., Dey, A. and Gayen, S. (2016) CRISPR/Cas9: an advanced tool for editing plant genomes. Transgenic Res. 25, 561–573.
    • (2016) Transgenic Res. , vol.25 , pp. 561-573
    • Samanta, M.K.1    Dey, A.2    Gayen, S.3
  • 45
    • 84928205754 scopus 로고    scopus 로고
    • High-throughput functional genomics using CRISPR-Cas9
    • Shalem, O., Sanjana, N.E. and Zhang, F. (2015) High-throughput functional genomics using CRISPR-Cas9. Nat. Rev. Genet. 16, 299–311.
    • (2015) Nat. Rev. Genet. , vol.16 , pp. 299-311
    • Shalem, O.1    Sanjana, N.E.2    Zhang, F.3
  • 47
    • 84974659966 scopus 로고    scopus 로고
    • CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii
    • Shin, S.E., Lim, J.M., Koh, H.G. et al. (2016) CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii. Sci. Rep. 6, 27810.
    • (2016) Sci. Rep. , vol.6 , pp. 27810
    • Shin, S.E.1    Lim, J.M.2    Koh, H.G.3
  • 48
    • 84874478059 scopus 로고    scopus 로고
    • Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases
    • Sizova, I., Greiner, A., Awasthi, M., Kateriya, S. and Hegemann, P. (2013) Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases. Plant J. 73, 873–882.
    • (2013) Plant J. , vol.73 , pp. 873-882
    • Sizova, I.1    Greiner, A.2    Awasthi, M.3    Kateriya, S.4    Hegemann, P.5
  • 50
    • 84874961299 scopus 로고    scopus 로고
    • Establishing oleaginous microalgae research models for consolidated bioprocessing of solar energy
    • Wang, D., Lu, Y., Huang, H. and Xu, J. (2012) Establishing oleaginous microalgae research models for consolidated bioprocessing of solar energy. Adv. Biochem. Eng. Biotechnol. 128, 69–84.
    • (2012) Adv. Biochem. Eng. Biotechnol. , vol.128 , pp. 69-84
    • Wang, D.1    Lu, Y.2    Huang, H.3    Xu, J.4
  • 51
    • 84893754953 scopus 로고    scopus 로고
    • Nannochloropsis genomes reveal evolution of microalgal oleaginous traits
    • Wang, D., Ning, K., Li, J. et al. (2014a) Nannochloropsis genomes reveal evolution of microalgal oleaginous traits. PLoS Genet. 10, e1004094.
    • (2014) PLoS Genet. , vol.10
    • Wang, D.1    Ning, K.2    Li, J.3
  • 52
    • 84899943431 scopus 로고    scopus 로고
    • Quantitative dynamics of triacylglycerol accumulation in microalgae populations at single-cell resolution revealed by Raman microspectroscopy
    • Wang, T., Ji, Y., Wang, Y., Jia, J., Li, J., Huang, S., Han, D., Hu, Q., Huang, W.E. and Xu, J. (2014b) Quantitative dynamics of triacylglycerol accumulation in microalgae populations at single-cell resolution revealed by Raman microspectroscopy. Biotechnol. Biofuels, 7, 58.
    • (2014) Biotechnol. Biofuels , vol.7 , pp. 58
    • Wang, T.1    Ji, Y.2    Wang, Y.3    Jia, J.4    Li, J.5    Huang, S.6    Han, D.7    Hu, Q.8    Huang, W.E.9    Xu, J.10
  • 53
    • 84921934205 scopus 로고    scopus 로고
    • Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew
    • Wang, Y., Cheng, X., Shan, Q., Zhang, Y., Liu, J., Gao, C. and Qiu, J.L. (2014c) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat. Biotechnol. 32, 947–951.
    • (2014) Nat. Biotechnol. , vol.32 , pp. 947-951
    • Wang, Y.1    Cheng, X.2    Shan, Q.3    Zhang, Y.4    Liu, J.5    Gao, C.6    Qiu, J.L.7
  • 54
    • 84881082114 scopus 로고    scopus 로고
    • Nannochloropsis plastid and mitochondrial phylogenomes reveal organelle diversification mechanism and intragenus phylotyping strategy in microalgae
    • Wei, L., Xin, Y., Wang, D. et al. (2013) Nannochloropsis plastid and mitochondrial phylogenomes reveal organelle diversification mechanism and intragenus phylotyping strategy in microalgae. BMC Genom. 14, 534.
    • (2013) BMC Genom. , vol.14 , pp. 534
    • Wei, L.1    Xin, Y.2    Wang, D.3
  • 55
    • 77955619699 scopus 로고    scopus 로고
    • An outlook on microalgal biofuels
    • Wijffels, R.H. and Barbosa, M.J. (2010) An outlook on microalgal biofuels. Science, 329, 796–799.
    • (2010) Science , vol.329 , pp. 796-799
    • Wijffels, R.H.1    Barbosa, M.J.2
  • 56
    • 64849088316 scopus 로고    scopus 로고
    • Green evolution and dynamic adaptations revealed by genomes of the marine picoeukaryotes Micromonas
    • Worden, A.Z., Lee, J.H., Mock, T. et al. (2009) Green evolution and dynamic adaptations revealed by genomes of the marine picoeukaryotes Micromonas. Science, 324, 268–272.
    • (2009) Science , vol.324 , pp. 268-272
    • Worden, A.Z.1    Lee, J.H.2    Mock, T.3
  • 57
    • 84943626749 scopus 로고    scopus 로고
    • Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions
    • Xiao, Y., Zhang, J., Cui, J., Yao, X., Sun, Z., Feng, Y. and Cui, Q. (2015a) Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions. Algal Res. 11, 55–62.
    • (2015) Algal Res. , vol.11 , pp. 55-62
    • Xiao, Y.1    Zhang, J.2    Cui, J.3    Yao, X.4    Sun, Z.5    Feng, Y.6    Cui, Q.7
  • 58
    • 84922342832 scopus 로고    scopus 로고
    • Controlling a toxic shock of pentachlorophenol (PCP) to anaerobic digestion using activated carbon addition
    • Xiao, Y.Y., de Araujo, C., Sze, C.C. and Stuckey, D.C. (2015b) Controlling a toxic shock of pentachlorophenol (PCP) to anaerobic digestion using activated carbon addition. Bioresour. Technol. 181, 303–311.
    • (2015) Bioresour. Technol. , vol.181 , pp. 303-311
    • Xiao, Y.Y.1    de Araujo, C.2    Sze, C.C.3    Stuckey, D.C.4
  • 59
    • 84879120322 scopus 로고    scopus 로고
    • Microalgal biomass production pathways: evaluation of life cycle environmental impacts
    • Zaimes, G.G. and Khanna, V. (2013) Microalgal biomass production pathways: evaluation of life cycle environmental impacts. Biotechnol. Biofuels, 6, 88.
    • (2013) Biotechnol. Biofuels , vol.6 , pp. 88
    • Zaimes, G.G.1    Khanna, V.2
  • 60
    • 84923196942 scopus 로고    scopus 로고
    • Raman-activated cell sorting based on dielectrophoretic single-cell trap and release
    • Zhang, P., Ren, L., Zhang, X., Shan, Y., Wang, Y., Ji, Y., Yin, H., Huang, W.E., Xu, J. and Ma, B. (2015a) Raman-activated cell sorting based on dielectrophoretic single-cell trap and release. Anal. Chem. 87, 2282–2289.
    • (2015) Anal. Chem. , vol.87 , pp. 2282-2289
    • Zhang, P.1    Ren, L.2    Zhang, X.3    Shan, Y.4    Wang, Y.5    Ji, Y.6    Yin, H.7    Huang, W.E.8    Xu, J.9    Ma, B.10
  • 61
    • 84940118897 scopus 로고    scopus 로고
    • Towards high-throughput microfluidic Raman-activated cell sorting
    • Zhang, Q., Zhang, P., Gou, H., Mou, C., Huang, W.E., Yang, M., Xu, J. and Ma, B. (2015b) Towards high-throughput microfluidic Raman-activated cell sorting. Analyst, 140, 6163–6174.
    • (2015) Analyst , vol.140 , pp. 6163-6174
    • Zhang, Q.1    Zhang, P.2    Gou, H.3    Mou, C.4    Huang, W.E.5    Yang, M.6    Xu, J.7    Ma, B.8
  • 62
    • 84880516193 scopus 로고    scopus 로고
    • Characterization of organic phosphorus in lake sediments by sequential fractionation and enzymatic hydrolysis
    • Zhu, Y., Wu, F., He, Z., Guo, J., Qu, X., Xie, F., Giesy, J.P., Liao, H. and Guo, F. (2013) Characterization of organic phosphorus in lake sediments by sequential fractionation and enzymatic hydrolysis. Environ. Sci. Technol. 47, 7679–7687.
    • (2013) Environ. Sci. Technol. , vol.47 , pp. 7679-7687
    • Zhu, Y.1    Wu, F.2    He, Z.3    Guo, J.4    Qu, X.5    Xie, F.6    Giesy, J.P.7    Liao, H.8    Guo, F.9


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.