Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

Nature Biotechnology

Volumn 32, Issue 9, 2014, Pages 947-951

  Wang, Yanpeng ^a   Cheng, Xi ^a   Shan, Qiwei ^a   Zhang, Yi ^a   Liu, Jinxing ^a   Gao, Caixia ^a   Qiu, Jin Long ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DNA SEQUENCES; FOOD PRODUCTS; FUNGI; GENES;

DOUBLE STRAND BREAKS; INDUCED MUTATIONS; METHODOLOGICAL FRAMEWORKS; MILDEW RESISTANCE; NATURAL POPULATION; NONHOMOLOGOUS END JOINING; SIMULTANEOUS EDITING; TRANSCRIPTION ACTIVATORS;

PLANTS (BOTANY);

CRISPR ASSOCIATED PROTEIN; PLANT DNA; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE;

ALLELE; ARTICLE; CONTROLLED STUDY; DOUBLE STRANDED DNA BREAK; GENE INSERTION; GENE TARGETING; GENETIC ENGINEERING; GENETIC RESISTANCE; HERITABILITY; HEXAPLOIDY; MUTATION; NATURAL POPULATION; NONHUMAN; PLANT GENETICS; POWDERY MILDEW; PRIORITY JOURNAL; TRANSGENIC PLANT; TRITICUM AESTIVUM; ASCOMYCETES; GENETICS; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; RNA EDITING; SEQUENCE HOMOLOGY; WHEAT;

ERYSIPHALES; TRITICUM AESTIVUM;

ALLELES; ASCOMYCOTA; BASE SEQUENCE; MOLECULAR SEQUENCE DATA; RNA EDITING; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRITICUM;

EID: 84921934205     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.2969     Document Type: Article

References (31)

1. Kim, H., Kim, J.S. A guide to genome engineering with programmable nucleases. Nat. Rev. Genet. 15, 321-334 (2014).
2. Boch, J. et al. Breaking the code of DNA binding specificity of TAL-type III effectors. Science 326, 1509-1512 (2009).
3. Moscou, M.J., Bogdanove, A.J. A simple cipher governs DNA recognition by TAL effectors. Science 326, 1501 (2009).
4. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823 (2013).
5. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826 (2013).
6. Elliott, C. et al. Functional conservation of wheat and rice Mlo orthologs in defense modulation to the powdery mildew fungus. Mol. Plant Microbe Interact. 15, 1069-1077 (2002).
7. Várallyay, É., Giczey, G., Burgyán, J. Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum. Arch. Virol. 157, 1345-1350 (2012).
8. Slade, A.J. et al. A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat. Biotechnol. 23, 75-81 (2005).
9. Dvorák, J. in Genetics and Genomics of the Triticeae Vol. 7. (eds. Muehlbauer, G.J., Feuillet, C.) 685-711 (Springer US, 2009).
10. Bibikova, M., Beumer, K., Trautman, J.K., Carroll, D. Enhancing gene targeting with designed zinc finger nucleases. Science 300, 764 (2003).
11. Symington, L.S., Gautier, J. Double-strand break end resection and repair pathway choice. Annu. Rev. Genet. 45, 247-271 (2011).
12. Voytas, D.F., Gao, C. Precision genome engineering and agriculture: opportunities and regulatory challenges. PLoS Biol. 12, e1001877 (2014).
13. Zhang, F. et al. High frequency targeted mutagenesis in Arabidopsis thaliana using zinc-finger nucleases. Proc. Natl. Acad. Sci. USA 107, 12028-12033 (2010).
14. Feng, Z. et al. Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis. Proc. Natl. Acad. Sci. USA 111, 4632-4637 (2014).
15. Christian, M., Qi, Y., Zhang, Y., Voytas, D.F. Targeted mutagenesis of Arabidopsis thaliana using engineered TAL effector nucleases. G3 (Bethesda) 3, 1697-1705 (2013).
16. Li, T. et al. High-efficiency TALEN-based gene editing produces disease-resistant rice. Nat. Biotechnol. 30, 390-392 (2012).
17. Shan, Q. et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat. Biotechnol. 31, 686-688 (2013).
18. Büschges, R. et al. The barley Mlo gene: A novel control element of plant pathogen resistance. Cell 88, 695-705 (1997).
19. Piffanelli, P. et al. A barley cultivation-associated polymorphism conveys resistance to powdery mildew. Nature 430, 887-891 (2004).
20. Consonni, C. et al. Conserved requirement for a plant host cell protein in powdery mildew pathogenesis. Nat. Genet. 38, 716-720 (2006).
21. Bai, Y. et al. Naturally occurring broad-spectrum powdery mildew resistance in a central american tomato accession is caused by loss of mlo function. Mol. Plant Microbe Interact. 21, 30-39 (2008).
22. Cermak, T. et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res. 39, e82 (2011).
23. Shan, Q. et al. Rapid and efficient gene modification in rice and brachypodium using TALENs. Mol. Plant 6, 1365-1368 (2013).
24. Christensen, A., Quail, P. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res. 5, 213-218 (1996).
25. Feng, Z. et al. Efficient genome editing in plants using a CRISPR/Cas system. Cell Res. 23, 1229-1232 (2013).
26. Xie, K., Yang, Y. RNA-guided genome editing in plants using a CRISPR-Cas system. Mol. Plant 6, 1975-1983 (2013).
27. McDonald, B.A., Linde, C. Pathogen population genetics, evolutionary potential, and durable resistance. Annu. Re. Phytopathol. 40, 349-379 (2002).
28. Zhang, Y. et al. Transcription activator-like effector nucleases enable efficient plant genome engineering. Plant Physiol. 161, 20-27 (2013).
29. Guerineau, F., Lucy, A., Mullineaux, P. Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts. Plant Mol. Biol. 18, 815-818 (1992).
30. Rasco-Gaunt, S. et al. Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment. J. Exp. Bot. 52, 865-874 (2001).
31. Hein, I. et al. Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley. Plant Physiol. 138, 2155-2164 (2005).