High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product

Bioscience, Biotechnology and Biochemistry

Volumn 78, Issue 5, 2014, Pages 812-817

  Li, Fengjuan ^a   Gao, Dawen ^a   Hu, Hanhua ^b  

^a HARBIN INSTITUTE OF TECHNOLOGY   (China)

^b INSTITUTE OF HYDROBIOLOGY   (China)

Author keywords

Cotransformation; Electroporation; GUS; Nannochloropsis transformation

Indexed keywords

DNA; EFFICIENCY; GENES; GENETIC ENGINEERING; PRODUCTION PLATFORMS;

COTRANSFORMATION; ELECTROPORATION; GUS; HIGH EFFICIENCY TRANSFORMATION; NANNOCHLOROPSIS TRANSFORMATION; NUCLEAR TRANSFORMATIONS; TRANSFORMATION EFFICIENCY; TRANSFORMATION PROTOCOLS;

POLYMERASE CHAIN REACTION;

ALGAE; NANNOCHLOROPSIS; NANNOCHLOROPSIS SALINA;

BIOFUEL; TUBULIN;

CELL NUCLEUS; CYTOLOGY; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; METABOLISM; MICROALGA; POLYMERASE CHAIN REACTION; PROCEDURES; PROMOTER REGION;

BIOFUELS; CELL NUCLEUS; GENETIC ENGINEERING; MICROALGAE; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; TRANSFORMATION, GENETIC; TUBULIN;

EID: 84912113111     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1080/09168451.2014.905184     Document Type: Article

References (35)

1. Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 2008;54:621-639.
2. Chisti Y. Biodiesel from microalgae. Biotechnol. Adv. 2007;25:294-306.
3. Weyer KM, Bush DR, Darzins A, Willson BD. Theoretical maximum algal oil production. Bioenerg. Res. 2010;3:204-213.
4. Andersen RA, Brett RW, Potter D, Sexton JP. Phylogeny of the Eustigmatophyceae based upon 18S rDNA, with emphasis on Nannochloropsis. Protist. 1998;149:61-74.
5. Borowitzka MA. Microalgae for aquaculture: opportunities and constraints. J. Appl. Phycol. 1997;9:393-401.
6. Sukenik A. Production of eicosapentaenoic acid by the marine eustigmatophyte Nannochloropsis. In: Cohen Z, editor. Chemicals from microalgae. London: Taylor & Francis; 1999. p. 41-56.
7. Patil V, Ka¨llqvist T, Olsen E, Vogt G, Gislerød HR. Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquacult. Int. 2007;15:1-9.
8. Rodolfi L. Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol. Bioeng. 2009;102:100-112.
9. Jinkerson RE, Radakovits R, Posewitz MC. Genomic insights from the oleaginous model alga Nannochloropsis gaditana. Bioengineered. 2013;4:37-43.
10. Tonon T, Harvey D, Larson TR, Graham IA. Long chain polyunsaturated fatty acid production and partitioning to triacylglycerols in four microalgae. Phytochemistry. 2002;61:15-24.
11. 2 concentration. Biotechnol. Lett. 2006;28:987-992.
12. Danielewicz MA, Anderson LA, Franz AK. Triacylglycerol profiling of marine microalgae by mass spectrometry. J. Lipid Res. 2011;52:2101-2108.
13. Simionato D, Sforza E. Acclimation of Nannochloropsis gaditana to different illumination regimes: effects on lipids accumulation. Bioresour. Technol. 2011;102:6026-6032.
14. Pal D, Khozin-Goldberg I, Didi-Cohen S, Solovchenko A, Batushansky A, Kaye Y, Sikron N, Samani T, Fait A, Boussiba S. Growth, lipid production and metabolic adjustments in the euryhaline eustigmatophyte Nannochloropsis oceanica CCALA 804 in response to osmotic downshift. Appl. Microbiol. Biotechnol. 2013;97:8291-8306.
15. Radakovits R, Jinkerson RE, Fuerstenberg SI, Tae H, Settlage RE, Boore JL, Posewitz MC. Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana. Nat. Commun. 2012;3:686.
16. Kilian O, Benemann CSE, Niyogi KK, Vick B. High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proc. Nat. Acad. Sci. USA. 2011;108:21265-21269.
17. Vieler A, Wu G, Tsai C-H, Bullard B, Cornish AJ, Harvey C, Reca I-B, Thornburg C, Achawanantakun R, Buehl CJ, Campbell MS, Cavalier D, Childs KL, Clark TJ, Deshpande R, Erickson E, Ferguson AA, Handee W, Kong Q, Li X, Liu B, Lundback S, Peng C, Roston RL, Sanjaya, Simpson JP, TerBus A, Warakanont J, Zauner S, Farre EM, Hegg EL, Jiang N, Kuo M-H, Lu Y, Niyogi KK, Ohlrogge J, Osteryoung KW, Shachar-Hill Y, Sears BB, Sun Y, Takahashi H, Yandell M, Shiu S-H, Benning C. Genome, functional gene annotation, and nuclear transformation of the Heterokont oleaginous alga Nannochloropsis oceanica CCMP1779. PLoS Genet. 2012;8:e1003064.
18. Hu H, Gao K. Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources. Biotechnol. Lett. 2003;25:421-425.
19. Guillard RRL. Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH, editors. Culture of marine invertebrate animals. New York, NY: Plenum Press; 1975. p. 29-60.
20. Harrison PJ, Waters RE, Taylor FJR. A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J. Phycol. 1980;16:28-35.
21. Stanier RY, Kunisawar R, Mandel M, Cohen-Bazire G. Purification and properties of unicellular blue-green algae (Order Chroococcales). Bacteriol. Rev. 1971;35:171-205.
22. Swofford DL. PAUP∗4.0 - phylogenetic analysis using parsimony (∗and other methods). Sunderland: Sinauer Associates; 1998.
23. Falciatore A, Casotti R, Leblanc C, Abrescia C, Bowler C. Transformation of nonselectable reporter genes in marine diatoms. Mar. Biotechnol. 1999;1:239-251.
24. Jefferson RA, Kavanagh TA, Bevan MW. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987;6:3901-3907.
25. Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB, Sanford JC. Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science. 1988;240:1534-1538.
26. Kindle KL. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Nat. Acad. Sci. USA. 1990;87:1228-1232.
27. Apt KE, Kroth-Pancic PG, Grossman AR. Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol. Gen. Genet. 1996;252:572-579.
28. Chen HL, Li SS, Huang R, Tsai H-J. Conditional production of a functional fish growth hormone in the transgenic line of Nannochloropsis oculata (Eustigmatophyceae). J. Phycol. 2008;44:768-776.
29. Li S-S, Tsai H-J. Transgenic microalgae as a non-antibiotic bactericide producer to defend against bacterial pathogen infection in the fish digestive tract. Fish Shellfish Immunol. 2009;26:316-325.
30. Kimura M. The neutral theory of molecular evolution. Cambridge: Cambridge University Press; 1984.
31. Suda S, Atsumi M, Miyashita H. Taxonomic characterization of a marine Nannochloropsis species, N. oceanica sp. nov. (Eustigmatophyceae). Phycologia. 2002;41:273-279.
32. Li S, Brisson M, He Y, Huang L. Delivery of a PCR amplified DNA fragment into cells: a model for using synthetic genes for gene therapy. Gene Ther. 1997;4:449-454.
33. Isalan M, Santori MI, Gonzalez C, Serrano L. Localized transfection on arrays of magnetic beads coated with PCR products. Nat. Methods. 2005;2:113-118.
34. Lu Y, Chen X, Wu Y, Wang Y, He Y, Wu Y. Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay. PLoS ONE. 2013;8:e57171.
35. Miyahara M, Aoi M, Inoue-Kashino N, Kashino Y, Ifuku K. Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation. Biosci. Biotechnol. Biochem. 2013;77:874-876.