Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies

Nature Biotechnology

Volumn 34, Issue 9, 2016, Pages 942-949

  Gohl, Daryl M ^a   Vangay, Pajau ^a   Garbe, John ^a   MacLean, Allison ^a   Hauge, Adam ^a,c   Becker, Aaron ^a   Gould, Trevor J ^a   Clayton, Jonathan B ^a   Johnson, Timothy J ^a   Hunter, Ryan ^a   Knights, Dan ^a,b   Beckman, Kenneth B ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b University of Minnesota   (United States)

^c University of Minnesota Informatics Institute ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ERRORS; GENES;

'CURRENT; AMPLICONS; COMMUNITY STANDARDS; EXPERIMENTAL BIAS; EXPERIMENTAL ERRORS; MARKER GENES; MICROBIAL COMMUNITIES; MICROBIOME; MULTIPLE METHODS; NEXT-GENERATION SEQUENCING;

MICROORGANISMS;

RNA 16S; GENETIC MARKER;

ACCURACY; AMPLICON; ARTICLE; BACTEROIDES VULGATUS; CAMPYLOBACTER JEJUNI; CHIMERA; CLINICAL ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; DEINOCOCCUS RADIODURANS; ESCHERICHIA COLI; HUMAN; MARKER GENE; MICROBIAL COMMUNITY; MICROBIOME; NEXT GENERATION SEQUENCING; NONHUMAN; POPULATION ABUNDANCE; PRIORITY JOURNAL; PROPIONIBACTERIUM ACNES; DNA REPLICATION; GENE LIBRARY; GENETIC MARKER; GENETICS; HIGH THROUGHPUT SEQUENCING; MICROFLORA; POLYMERASE CHAIN REACTION; PRACTICE GUIDELINE; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; STANDARDS;

DNA REPLICATION; GENE LIBRARY; GENETIC MARKERS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MICROBIOTA; POLYMERASE CHAIN REACTION; PRACTICE GUIDELINES AS TOPIC; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84986538850     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3601     Document Type: Article

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